Long non‑coding RNA RP11‑480I12.5 promotes cervical carcinoma progression by regulating the Wnt/β‑catenin signaling pathway

Zhang,Li; Li,Yaqin; Sona,Lina

doi:10.3892/ol.2019.11120

Long non‑coding RNA RP11‑480I12.5 promotes cervical carcinoma progression by regulating the Wnt/β‑catenin signaling pathway

Authors:
- Li Zhang
- Yaqin Li
- Lina Sona
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Affiliations: Department of Gynaecology and Obstetrics, Jinan Women and Children Health Hospital, Jinan, Shandong 250001, P.R. China, Medical Reproductive Center, The First Affiliated Hospital of Soochow University, Soochow, Jiangsu 215006, P.R. China
Published online on: November 19, 2019 https://doi.org/10.3892/ol.2019.11120
Pages: 469-475
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The long non‑coding RNA (lncRNA), RP11‑480I12.5 is one of the most dysregulated lncRNAs, which is believed to contribute to the progression of cervical carcinoma (CC); however, the exact function of RP11‑480I12.5 in human CC remains unknown. The present study aimed to investigate the function and underlying molecular mechanism of RP11‑480I12.5 in CC. First, reverse transcription‑quantitative PCR was implemented in order to detect differences in the expression of RP11‑480I12.5 between normal and CC tissues. The present study used in vitro analysis to establish RP11‑480I12.5 stable knockdown and overexpressing cell lines, in order to investigate the function and potential molecular mechanism of RP11‑480I12.5 in the progression of CC. RP11‑480I12.5 was upregulated in CC tissue compared with normal tissue. Furthermore, RP11‑480I12.5 was associated with clinical stage, tumor size and lymph node metastasis. RP11‑480I12.5 promoted the proliferation, migration and invasion of CC cell lines. Subsequently, the present study investigated the association between RP11‑480I12.5 and the epithelial‑to‑mesenchymal transition (EMT) and Wnt/β‑catenin pathways. RP11‑480I12.5 promoted EMT through the Wnt/β‑catenin pathway. Overall, the results of the present study demonstrate that RP11‑480I12.5 promotes cercical cancer cell migration, invasion and EMT through the Wnt/β‑catenin pathway.

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