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Article Open Access

Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1

  • Authors:
    • Zhihu Liu
    • Yan Qin
    • Shuxiao Dong
    • Xiao Chen
    • Zhibin Huo
    • Zhongguang Zhen
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China, Surgical Department of Gastrointestinal Neoplasms, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China, Obstetrical Department, Xingtai Third Hospital, Xingtai, Hebei 054000, P.R. China, Department of Anesthesiology, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 663-670
    |
    Published online on: November 28, 2019
       https://doi.org/10.3892/ol.2019.11151
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Abstract

Chemotherapy resistance poses a major challenge for the clinical treatment of colorectal cancer, therefore, the aim of the present study was to examine its underlying mechanisms. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to determine the microRNA (miRNA)/mRNA and protein expression levels, respectively. A dual luciferase assay was conducted for verification of the interaction between miR‑106a and 3'untranslated region (UTR) of Forkhead box Q1 (FOXQ1). Cell viability was assessed using an MTT assay. In the present study, it was demonstrated that miR‑106a is involved in regulating oxaliplatin sensitivity of colorectal cancer. Transfection of miR‑106a mimics slightly inhibited colorectal cancer cell growth and sensitized colorectal cancer cells to oxaliplatin exposure. In addition, miR‑106a overexpression induced a decrease of FOXQ1 at mRNA and protein levels in colorectal cancer cells. The enhanced expression of miR‑106a also increased the expression of Wnt target genes, including vascular endothelial growth factor‑A and matrix metallopeptidase 2, which were reported to be regulated by FOXQ1. It was predicted and validated that miR‑106a could repress FOXQ1 expression via direct binding to 3'UTR. Elevation of miR‑106a and a decrease of FOXQ1 expression levels were detected in tumor tissues from patients with oxaliplatin‑sensitive colorectal cancer, compared with patients with oxaliplatin‑resistant colorectal cancer. Furthermore, there was a significant association between miR‑106a and FOXQ1 mRNA levels. In conclusion, the present study demonstrated that miR‑106a increased oxaliplatin sensitivity of colorectal cancer cells through direct repression of FOXQ1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Qin Y, Dong S, Chen X, Huo Z and Zhen Z: Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1. Oncol Lett 19: 663-670, 2020.
APA
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., & Zhen, Z. (2020). Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1. Oncology Letters, 19, 663-670. https://doi.org/10.3892/ol.2019.11151
MLA
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., Zhen, Z."Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1". Oncology Letters 19.1 (2020): 663-670.
Chicago
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., Zhen, Z."Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1". Oncology Letters 19, no. 1 (2020): 663-670. https://doi.org/10.3892/ol.2019.11151
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Qin Y, Dong S, Chen X, Huo Z and Zhen Z: Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1. Oncol Lett 19: 663-670, 2020.
APA
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., & Zhen, Z. (2020). Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1. Oncology Letters, 19, 663-670. https://doi.org/10.3892/ol.2019.11151
MLA
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., Zhen, Z."Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1". Oncology Letters 19.1 (2020): 663-670.
Chicago
Liu, Z., Qin, Y., Dong, S., Chen, X., Huo, Z., Zhen, Z."Overexpression of miR‑106a enhances oxaliplatin sensitivity of colorectal cancer through regulation of FOXQ1". Oncology Letters 19, no. 1 (2020): 663-670. https://doi.org/10.3892/ol.2019.11151
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