Long non‑coding RNA TTN‑AS1 promotes breast cancer cell migration and invasion via sponging miR‑140‑5p Retraction in /10.3892/ol.2020.12018

Xue,Jie; Zhang,Zhixia; Li,Xiaona; Ren,Qingfang; Wang,Qinghua

doi:10.3892/ol.2019.11222

Long non‑coding RNA TTN‑AS1 promotes breast cancer cell migration and invasion via sponging miR‑140‑5p

Retraction in: /10.3892/ol.2020.12018

Authors:
- Jie Xue
- Zhixia Zhang
- Xiaona Li
- Qingfang Ren
- Qinghua Wang
View Affiliations

Affiliations: Department of Breast Surgery, Linyi Cancer Hospital, Linyi, Shandong 276000, P.R. China, Department of Medical Oncology, Linyi Cancer Hospital, Linyi, Shandong 276000, P.R. China
Published online on: December 17, 2019 https://doi.org/10.3892/ol.2019.11222
Pages: 1255-1260
Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Breast cancer (BC) is one of the most common fatal cancers. Recent studies have identified the vital role of long non‑coding RNAs (lncRNAs) in the development and progression of BC. In this investigation, lncRNA TTN‑AS1 was studied to identify its function in the metastasis of BC. TTN‑AS1 expression of tissues was detected by real‑time quantitative polymerase chain reaction (RT‑qPCR) in 56 BC patients. Wound healing assay and transwell assay were used to observe the biological behavior changes of BC cells through gain or loss of TTN‑AS1. In addition, luciferase assays and RNA immunoprecipitation (RIP) assay were performed to discover the potential targets of TTN‑AS1 in BC cells. TTN‑AS1 expression level in BC samples was higher than that of adjacent tissue. Besides, the ability of cell migration and invasion of BC cells was inhibited after TTN‑AS1 was silenced, while cell migration and cell invasion of BC cells were promoted after TTN‑AS1 was overexpressed. In addition, miR‑140‑5p was upregulated after silencing of TTN‑AS1 in BC cells, while miR‑140‑5p was downregulated after overexpression of TTN‑AS1 in BC cells. Furthermore, luciferase assays and RIP assay showed that miR‑140‑5p was a direct target of TTN‑AS1 in BC. Our study uncovered a new oncogene in BC and suggests that TTN‑AS1 enhances BC cell migration and invasion via sponging miR‑140‑5p, which provides a novel therapeutic target for BC patients.

View Figures

View References

1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI
2	Luo X, Song Y, Tang L, Sun DH and Ji DG: lncRNA SNHG7 promotes development of breast cancer by regulating microRNA-186. Eur Rev Med Pharmacol Sci. 22:7788–7797. 2018.PubMed/NCBI
3	Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI
4	Dong Z, Yang P, Qiu X, Liang S, Guan B, Yang H, Li F, Sun L, Liu H, Zou G, et al: KCNQ1OT1 facilitates progression of non-small-cell lung carcinoma via modulating miRNA-27b-3p/HSP90AA1 axis. J Cell Physiol. 234:11304–11314. 2019. View Article : Google Scholar : PubMed/NCBI
5	Liu D, Li Y, Luo G, Xiao X, Tao D, Wu X, Wang M, Huang C, Wang L, Zeng F, et al: lncRNA SPRY4-IT1 sponges miR-101-3p to promote proliferation and metastasis of bladder cancer cells through up-regulating EZH2. Cancer Lett. 388:281–291. 2017. View Article : Google Scholar : PubMed/NCBI
6	Song J, Wu X, Liu F, Li M, Sun Y, Wang Y, Wang C, Zhu K, Jia X, Wang B, et al: Long non-coding RNA PVT1 promotes glycolysis and tumor progression by regulating miR-497/HK2 axis in osteosarcoma. Biochem Biophys Res Commun. 490:217–224. 2017. View Article : Google Scholar : PubMed/NCBI
7	Terashima M, Ishimura A, Wanna-Udom S and Suzuki T: MEG8 long non-coding RNA contributes to epigenetic progression of the epithelial-mesenchymal transition of lung and pancreatic cancer cells. J Biol Chem. 293:18016–18030. 2018. View Article : Google Scholar : PubMed/NCBI
8	Wang K, Liu F, Liu CY, An T, Zhang J, Zhou LY, Wang M, Dong YH, Li N, Gao JN, et al: The long non-coding RNA NRF regulates programmed necrosis and myocardial injury during ischemia and reperfusion by targeting miR-873. Cell Death Differ. 23:1394–1405. 2016. View Article : Google Scholar : PubMed/NCBI
9	Chen X, Liu S, Zhao X, Ma X, Gao G, Yu L, Yan D, Dong H and Sun W: Long noncoding RNA ILF3-AS1 promotes cell proliferation, migration, and invasion via negatively regulating miR-200b/a/429 in melanoma. Biosci Rep. 37(pii): BSR201710312017. View Article : Google Scholar : PubMed/NCBI
10	Liu C, Pan C, Cai Y and Wang H: Interplay between long non-coding RNA ZEB1-AS1 and miR-200s regulates osteosarcoma cell proliferation and migration. J Cell Biochem. 118:2250–2260. 2017. View Article : Google Scholar : PubMed/NCBI
11	Zhang D, Cao J, Zhong Q, Zeng L, Cai C, Lei L, Zhang W and Liu F: Long non-coding RNA PCAT-1 promotes invasion and metastasis via the miR-129-5p-HMGB1 signaling pathway in hepatocellular carcinoma. Biomed Pharmacother. 95:1187–1193. 2017. View Article : Google Scholar : PubMed/NCBI
12	Lee J, Jung JH, Chae YS, Park HY, Kim WW, Lee SJ, Jeong JH and Kang SH: Long non-coding RNA snaR regulates proliferation, migration and invasion of triple-negative breast cancer cells. Anticancer Res. 36:6289–6295. 2016. View Article : Google Scholar : PubMed/NCBI
13	Liu G, Hu X and Zhou G: Long non-coding RNA OR3A4 promotes proliferation and migration in breast cancer. Biomed Pharmacother. 96:426–433. 2017. View Article : Google Scholar : PubMed/NCBI
14	Yan M, Zhang L, Li G, Xiao S, Dai J and Cen X: Long non-coding RNA linc-ITGB1 promotes cell migration and invasion in human breast cancer. Biotechnol Appl Biochem. 64:5–13. 2017. View Article : Google Scholar : PubMed/NCBI
15	Lu P, Gu Y, Li L, Wang F, Yang X and Yang Y: Long non-coding RNA CAMTA1 promotes proliferation and mobility of the human breast cancer cell line MDA-MB-231 via targeting miR-20b. Oncol Res. 26:625–635. 2018. View Article : Google Scholar : PubMed/NCBI
16	Chen P, Wang R, Yue Q and Hao M: Long non-coding RNA TTN-AS1 promotes cell growth and metastasis in cervical cancer via miR-573/E2F3. Biochem Biophys Res Commun. 503:2956–2962. 2018. View Article : Google Scholar : PubMed/NCBI
17	Fang Z, Yin S, Sun R, Zhang S, Fu M, Wu Y, Zhang T, Khaliq J and Li Y: miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1. Mol Cancer. 16:1392017. View Article : Google Scholar : PubMed/NCBI
18	Yang H, Fang F, Chang R and Yang L: MicroRNA-140-5p suppresses tumor growth and metastasis by targeting transforming growth factor β receptor 1 and fibroblast growth factor 9 in hepatocellular carcinoma. Hepatology. 58:205–217. 2013. View Article : Google Scholar : PubMed/NCBI
19	Su Y, Xiong J, Hu J, Wei X, Zhang X and Rao L: MicroRNA-140-5p targets insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) to suppress cervical cancer growth and metastasis. Oncotarget. 7:68397–68411. 2016. View Article : Google Scholar : PubMed/NCBI
20	Lu Y, Qin T, Li J, Wang L, Zhang Q, Jiang Z and Mao J: MicroRNA-140-5p inhibits invasion and angiogenesis through targeting VEGF-A in breast cancer. Cancer Gene Ther. 24:386–392. 2017. View Article : Google Scholar : PubMed/NCBI