Open Access

BMP4 induces asymmetric cell division in human glioma stem-like cells

  • Authors:
    • Motofumi Koguchi
    • Yukiko Nakahara
    • Hiroshi Ito
    • Tomihiro Wakamiya
    • Fumitaka Yoshioka
    • Atsushi Ogata
    • Kohei Inoue
    • Jun Masuoka
    • Hideki Izumi
    • Tatsuya Abe
  • View Affiliations

  • Published online on: December 20, 2019     https://doi.org/10.3892/ol.2019.11231
  • Pages: 1247-1254
  • Copyright: © Koguchi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma (GBM) is a malignant tumor with a high recurrence rate and has very poor prognosis in humans. The median survival is still <2 years. Therefore, a new treatment strategy should be established. Recently, this cancer has been thought to be heterogeneous, consisting of cancer stem cells (CSCs) that are self-renewable, multipotent, and treatment resistant. So various strategies targeting glioma stem-like cells (GSCs) have been investigated. This study focused on strategies targeting GSCs through the induction of differentiation using bone morphogenetic protein 4 (BMP4). The expression of CD133, a cancer stem cell marker, under BMP4 treatment in GSCs was examined using flow cytometry, western blotting, and quantitative PCR. Immunofluorescent staining of GSCs was also performed to examine the type of cell division: asymmetric cell division (ACD) or symmetric cell division (SCD). We obtained the following results. The BMP4 treatment caused downregulation of CD133 expression. Moreover, it induced ACD in GSCs. While the ACD ratio was 23% without BMP4 treatment, it was 38% with BMP4 treatment (P=0.004). Furthermore, the tumor sphere assay demonstrated that BMP4 suppresses self-renewal ability. In conclusion, these findings may provide a new perspective on how BMP4 treatment reduces the tumorigenicity of GSCs.
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February-2020
Volume 19 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Koguchi M, Nakahara Y, Ito H, Wakamiya T, Yoshioka F, Ogata A, Inoue K, Masuoka J, Izumi H, Abe T, Abe T, et al: BMP4 induces asymmetric cell division in human glioma stem-like cells. Oncol Lett 19: 1247-1254, 2020
APA
Koguchi, M., Nakahara, Y., Ito, H., Wakamiya, T., Yoshioka, F., Ogata, A. ... Abe, T. (2020). BMP4 induces asymmetric cell division in human glioma stem-like cells. Oncology Letters, 19, 1247-1254. https://doi.org/10.3892/ol.2019.11231
MLA
Koguchi, M., Nakahara, Y., Ito, H., Wakamiya, T., Yoshioka, F., Ogata, A., Inoue, K., Masuoka, J., Izumi, H., Abe, T."BMP4 induces asymmetric cell division in human glioma stem-like cells". Oncology Letters 19.2 (2020): 1247-1254.
Chicago
Koguchi, M., Nakahara, Y., Ito, H., Wakamiya, T., Yoshioka, F., Ogata, A., Inoue, K., Masuoka, J., Izumi, H., Abe, T."BMP4 induces asymmetric cell division in human glioma stem-like cells". Oncology Letters 19, no. 2 (2020): 1247-1254. https://doi.org/10.3892/ol.2019.11231