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Article Open Access

lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines

  • Authors:
    • Xue Wu
    • Yiqun Yuan
    • Ruiqi Ma
    • Binbin Xu
    • Rui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai 200031, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1505-1515
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    Published online on: December 23, 2019
       https://doi.org/10.3892/ol.2019.11240
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Abstract

Previous studies have demonstrated that the long non‑coding RNA, small nucleolar RNA host gene 7 (SNHG7) plays an important role in several types of cancer; however, its role in the development of uveal melanoma (UM) remains unclear. The present study investigated the effect of SNHG7 on the prognosis of UM, as well as on cell proliferation, cell cycle and apoptosis of UM cell lines. Furthermore, the present study aimed to determine the molecular mechanisms underlying these effects. The association between SNHG7 and prognosis of UM was analyzed using detailed SNHG7 mRNA expression data and clinical information from The Cancer Genome Atlas database. Reverse transcription‑quantitative PCR was used in order to detect the differential expression of SNHG7 in UM tissues and cell lines. Cell proliferation was detected using Cell Counting Kit‑8 assays, following overexpression of SNHG7. A cell cycle assay was performed using propidium iodide/RNase staining. An apoptosis assay was performed using the Annexin‑V‑Fluorescein isothiocyanate apoptosis detection kit. The expression of enhancer of zeste homolog 2 (EZH2) was measured via western blotting. The results of the present study indicated that low expression of SNHG7 was associated with poor prognosis. Furthermore, increasing the expression of SNHG7 inhibited the proliferation of UM cells, suppressed cell cycle progression and promoted apoptosis. Western blot analysis results revealed that overexpression of SNHG7 downregulated EZH2 protein expression levels in UM cell lines. The results of the present study demonstrated that SNHG7 inhibited malignant transformation of UM cells by regulating EZH2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Wu X, Yuan Y, Ma R, Xu B and Zhang R: lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines. Oncol Lett 19: 1505-1515, 2020.
APA
Wu, X., Yuan, Y., Ma, R., Xu, B., & Zhang, R. (2020). lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines. Oncology Letters, 19, 1505-1515. https://doi.org/10.3892/ol.2019.11240
MLA
Wu, X., Yuan, Y., Ma, R., Xu, B., Zhang, R."lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines". Oncology Letters 19.2 (2020): 1505-1515.
Chicago
Wu, X., Yuan, Y., Ma, R., Xu, B., Zhang, R."lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines". Oncology Letters 19, no. 2 (2020): 1505-1515. https://doi.org/10.3892/ol.2019.11240
Copy and paste a formatted citation
x
Spandidos Publications style
Wu X, Yuan Y, Ma R, Xu B and Zhang R: lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines. Oncol Lett 19: 1505-1515, 2020.
APA
Wu, X., Yuan, Y., Ma, R., Xu, B., & Zhang, R. (2020). lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines. Oncology Letters, 19, 1505-1515. https://doi.org/10.3892/ol.2019.11240
MLA
Wu, X., Yuan, Y., Ma, R., Xu, B., Zhang, R."lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines". Oncology Letters 19.2 (2020): 1505-1515.
Chicago
Wu, X., Yuan, Y., Ma, R., Xu, B., Zhang, R."lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines". Oncology Letters 19, no. 2 (2020): 1505-1515. https://doi.org/10.3892/ol.2019.11240
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