Open Access

Cardamonin as a potential treatment for melanoma induces human melanoma cell apoptosis

  • Authors:
    • Yuyang Yue
    • Lijuan Liu
    • Peipei Liu
    • Yuting Li
    • Haitao Lu
    • Yanjia Li
    • Guoqiang Zhang
    • Xinsuo Duan
  • View Affiliations

  • Published online on: December 23, 2019     https://doi.org/10.3892/ol.2019.11242
  • Pages: 1393-1399
  • Copyright: © Yue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

2',4'‑dihydroxy‑6'‑methoxychalcone (cardamonin) is a natural compound with anti‑proliferative effects on several cancer types including nasopharyngeal carcinoma. The effects of cardamonin on melanoma cells are unknown. The present study investigated the anti‑proliferative effect of cardamonin on human melanoma cell lines (M14 and A375), and the underlying apoptosis inducing mechanisms. MTS assay showed that cardamonin inhibited M14 cells viability, and a reduction of the M14 cell density was also observed. Flow cytometry showed that cardamonin induced M14 cells apoptosis in a dose‑dependent manner. Western blot analysis showed protein expression in M14 and A375; the pro‑apoptotic protein BAX was upregulated, while the anti‑apoptotic protein B‑cell lymphoma‑2 was downregulated. The protein expression of cleaved caspase‑8, ‑9 and cleaved poly (ADP‑ribose) polymerase was increased, whereas P65 was decreased. Furthermore, cardamonin inhibited M14 cell migration. These findings suggest that cardamonin may be a novel anticancer treatment for human melanoma.
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February-2020
Volume 19 Issue 2

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Spandidos Publications style
Yue Y, Liu L, Liu P, Li Y, Lu H, Li Y, Zhang G and Duan X: Cardamonin as a potential treatment for melanoma induces human melanoma cell apoptosis. Oncol Lett 19: 1393-1399, 2020
APA
Yue, Y., Liu, L., Liu, P., Li, Y., Lu, H., Li, Y. ... Duan, X. (2020). Cardamonin as a potential treatment for melanoma induces human melanoma cell apoptosis. Oncology Letters, 19, 1393-1399. https://doi.org/10.3892/ol.2019.11242
MLA
Yue, Y., Liu, L., Liu, P., Li, Y., Lu, H., Li, Y., Zhang, G., Duan, X."Cardamonin as a potential treatment for melanoma induces human melanoma cell apoptosis". Oncology Letters 19.2 (2020): 1393-1399.
Chicago
Yue, Y., Liu, L., Liu, P., Li, Y., Lu, H., Li, Y., Zhang, G., Duan, X."Cardamonin as a potential treatment for melanoma induces human melanoma cell apoptosis". Oncology Letters 19, no. 2 (2020): 1393-1399. https://doi.org/10.3892/ol.2019.11242