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Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression

  • Authors:
    • Weihua Wang
    • Leilei Zhan
    • Dongqi Guo
    • Yanju Xiang
    • Muxing Tian
    • Yu Zhang
    • Hong Wu
    • Yaxun Wei
    • Ganglong Ma
    • Zhanjiang Han
  • View Affiliations / Copyright

    Affiliations: College of Life Science, Tarim University, Alar, Xinjiang 843300, P.R. China, Center for Genome Analysis, ABLife Inc., Wuhan, Hubei 430075, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2777-2787
    |
    Published online on: January 4, 2019
       https://doi.org/10.3892/ol.2019.9887
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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs of 18‑25 nucleotides that modulate gene expression at the post‑transcriptional level. Grape seed proanthocyanidins (GSPs), which are biologically active components in grape seeds, have been demonstrated to exhibit anticancer effects. The current study investigated whether GSPs can regulate miRNA expression and the possible anticancer molecular mechanisms of GSPs. Pancreatic cancer (PC) cell samples, SS3, SS12 and SS24, were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control samples, SC3, SC12 and SC24, were also prepared. Using miRNA‑seq, transcriptome analysis identified 24, 83 and 83 differentially expressed (DE) miRNAs in SS3 vs. SC3, SS12 vs. SC12 and SS24 vs. SC24, respectively. This indicated that treatment with GSPs could modulate the expression of miRNAs. Subsequently, 74, 598 and 1,204 target genes for the three sets of DE miRNAs were predicted. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that multiple target genes were associated with the proliferation and apoptosis of PC cells. In addition, a network was constructed of the DE miRNAs and the target genes associated with PC. The associations identified suggested that treatment with GSPs may inhibit the proliferation of PC cells through the modulation of miRNA expression.
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View References

1 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Kato M and Slack FJ: microRNAs: Small molecules with big roles-C. Elegans to human cancer. Biol Cell. 100:71–81. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Vasudevan S, Tong Y and Steitz JA: Switching from repression to activation: MicroRNAs can up-regulate translation. Science. 318:1931–1934. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Li Y, Qiu C, Tu J, Geng B, Yang J, Jiang T and Cui Q: HMDD v2.0: A database for experimentally supported human microRNA and disease associations. Nucleic Acids Res. 42:D1070–D1074. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Kozomara A and Griffiths-Jones S: miRBase: Integrating microRNA annotation and deep-sequencing data. Nucleic Acids Res. 39:D152–D157. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Zhang R and Su B: Small but influential: The role of microRNAs on gene regulatory network and 3′UTR evolution. J Genet Genomics. 36:1–6. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Tan L, Yu JT, Tan MS, Liu QY, Wang HF, Zhang W, Jiang T and Tan L: Genome-wide serum microRNA expression profiling identifies serum biomarkers for Alzheimer's disease. J Alzheimers Dis. 40:1017–1027. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Dangwal S and Thum T: microRNA therapeutics in cardiovascular disease models. Annu Rev Pharmacol Toxicol. 54:185–203. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Iorio MV, Ferracin M, Liu CG, Veronese A, Spizzo R, Sabbioni S, Magri E, Pedriali M, Fabbri M, Campiglio M, et al: MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 65:7065–7070. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Cheng CJ, Bahal R, Babar IA, Pincus Z, Barrera F, Liu C, Svoronos A, Braddock DT, Glazer PM, Engelman DM, et al: MicroRNA silencing for cancer therapy targeted to the tumour microenvironment. Nature. 518:107–110. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Arola-Arnal A and Blade C: Proanthocyanidins modulate microRNA expression in human HepG2 cells. PLoS One. 6:e259822011. View Article : Google Scholar : PubMed/NCBI

14 

Bansode RR, Khatiwada JR, Losso JN and Williams LL: Targeting microRNA in cancer using plant-based proanthocyanidins. Diseases. 4:E212016. View Article : Google Scholar : PubMed/NCBI

15 

Chung YC, Huang CC, Chen CH, Chiang HC, Chen KB, Chen YJ, Liu CL, Chuang LT, Liu M and Hsu CP: Grape-seed procyanidins inhibit the in vitro growth and invasion of pancreatic carcinoma cells. Pancreas. 41:447–454. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Prasad R, Vaid M and Katiyar SK: Grape proanthocyanidin inhibit pancreatic cancer cell growth in vitro and in vivo through induction of apoptosis and by targeting the PI3K/Akt pathway. PLoS One. 7:e430642012. View Article : Google Scholar : PubMed/NCBI

17 

Prasad R and Katiyar SK: Grape seed proanthocyanidins inhibit migration potential of pancreatic cancer cells by promoting mesenchymal-to-epithelial transition and targeting NF-κB. Cancer Lett. 334:118–126. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Wang WH, Zhan LL, Guo DQ, Xiang YJ, Zhang Y, Tian MX and and Han ZJ: Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins. Oncol Lett. 17:1741–1749. 2019.

19 

Langmead B, Trapnell C, Pop M and Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10:R252009. View Article : Google Scholar : PubMed/NCBI

20 

Kozomara A and Griffiths-Jones S: miRBase: Annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 42:D68–D73. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Friedlander MR, Chen W, Adamidi C, Maaskola J, Einspanier R, Knespel S and Rajewsky N: Discovering microRNAs from deep sequencing data using miRDeep. Nat Biotechnol. 26:407–415. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Robinson MD, McCarthy DJ and Smyth GK: edgeR: A bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 26:139–140. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Krek A, Grun D, Poy MN, Wolf R, Rosenberg L, Epstein EJ, MacMenamin P, da Piedade I, Gunsalus KC, Stoffel M and Rajewsky N: Combinatorial microRNA target predictions. Nat Genet. 37:495–500. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Lee Y: Cancer chemopreventive potential of procyanidin. Toxicol Res. 33:273–282. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Serrano J, Puupponen-Pimia R, Dauer A, Aura AM and Saura-Calixto F: Tannins: Current knowledge of food sources, intake, bioavailability and biological effects. Mol Nutr Food Res. 53 Suppl 2:S310–S329. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Kidd PM: Bioavailability and activity of phytosome complexes from botanical polyphenols: The silymarin, curcumin, green tea, and grape seed extracts. Altern Med Rev. 14:226–246. 2009.PubMed/NCBI

30 

Bagchi D, Swaroop A, Preuss HG and Bagchi M: Free radical scavenging, antioxidant and cancer chemoprevention by grape seed proanthocyanidin: An overview. Mutat Res. 768:69–73. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Ma J, Fang B, Ma C, Pang H, Zeng F and Xia J: Proanthocyanidins inhibit pancreatic cancer AsPC-1 cell growth and migration through up-regulation of let-7a. Nan Fang Yi Ke Da Xue Xue Bao. 35:1110–1115. 2015.(In Chinese). PubMed/NCBI

32 

Prasad R and Katiyar SK: Down-regulation of miRNA-106b inhibits growth of melanoma cells by promoting G1-phase cell cycle arrest and reactivation of p21/WAF1/Cip1 protein. Oncotarget. 5:10636–10649. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang W, Zhan L, Guo D, Xiang Y, Tian M, Zhang Y, Wu H, Wei Y, Ma G, Han Z, Han Z, et al: Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncol Lett 17: 2777-2787, 2019.
APA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y. ... Han, Z. (2019). Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncology Letters, 17, 2777-2787. https://doi.org/10.3892/ol.2019.9887
MLA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y., Wu, H., Wei, Y., Ma, G., Han, Z."Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression". Oncology Letters 17.3 (2019): 2777-2787.
Chicago
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y., Wu, H., Wei, Y., Ma, G., Han, Z."Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression". Oncology Letters 17, no. 3 (2019): 2777-2787. https://doi.org/10.3892/ol.2019.9887
Copy and paste a formatted citation
x
Spandidos Publications style
Wang W, Zhan L, Guo D, Xiang Y, Tian M, Zhang Y, Wu H, Wei Y, Ma G, Han Z, Han Z, et al: Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncol Lett 17: 2777-2787, 2019.
APA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y. ... Han, Z. (2019). Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncology Letters, 17, 2777-2787. https://doi.org/10.3892/ol.2019.9887
MLA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y., Wu, H., Wei, Y., Ma, G., Han, Z."Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression". Oncology Letters 17.3 (2019): 2777-2787.
Chicago
Wang, W., Zhan, L., Guo, D., Xiang, Y., Tian, M., Zhang, Y., Wu, H., Wei, Y., Ma, G., Han, Z."Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression". Oncology Letters 17, no. 3 (2019): 2777-2787. https://doi.org/10.3892/ol.2019.9887
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