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Article Open Access

Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition

Corrigendum in: /10.3892/ol.2019.10369
  • Authors:
    • Wenjie Xie
    • Xin Liu
    • Qiang Chen
    • Ming Ma
    • Xiaorong Yang
    • Binbin Gong
    • Ting Sun
    • Jie Chen
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Department of Blood Transfusion, Jiangxi Provincial Children's Hospital, Nanchang, Jiangxi 330000, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3173-3180
    |
    Published online on: January 25, 2019
       https://doi.org/10.3892/ol.2019.9967
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Abstract

Long non‑coding RNAs (lncRNAs) have been demonstrated to serve vital roles in renal cell carcinoma (RCC) development. Gastric carcinoma high expressed transcript 1 (GHET1) regulates numerous biological processes in cancer cells. However, the biological role of GHET1 in RCC has not yet been identified. This study aimed to investigate the role of GHET1 in RCC. In the present study, the expression of GHET1 in RCC tissues and the 786‑O, A498 and 293 cell lines was assessed by reverse transcription‑quantitative polymerase chain reaction. Cell Counting Kit‑8, colony formation and cell scratch assays were used to determine the effects of GHET1 on tumorigenesis. Western blotting was performed to examine the effect of GHET1 on epithelial‑mesenchymal transition (EMT) in RCC cells. GHET1 expression was significantly increased in the RCC samples in comparison with adjacent tissues. High expression levels of GHET1 were associated with distant metastasis and clinical stage severity, thus, high GHET1 expression may serve as a predictor for a poor prognosis. In addition, RCC cells presented higher GHET1 mRNA and protein expression levels compared with in 293 cells. Furthermore, silencing GHET1 suppressed cell growth, weakened cell migration and inhibited EMT of RCC cells in vitro. In conclusion, the present study suggested that GHET1 may be considered a therapeutic target for the treatment or prevention of RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Xie W, Liu X, Chen Q, Ma M, Yang X, Gong B, Sun T and Chen J: Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369. Oncol Lett 17: 3173-3180, 2019.
APA
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B. ... Chen, J. (2019). Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369. Oncology Letters, 17, 3173-3180. https://doi.org/10.3892/ol.2019.9967
MLA
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B., Sun, T., Chen, J."Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369". Oncology Letters 17.3 (2019): 3173-3180.
Chicago
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B., Sun, T., Chen, J."Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369". Oncology Letters 17, no. 3 (2019): 3173-3180. https://doi.org/10.3892/ol.2019.9967
Copy and paste a formatted citation
x
Spandidos Publications style
Xie W, Liu X, Chen Q, Ma M, Yang X, Gong B, Sun T and Chen J: Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369. Oncol Lett 17: 3173-3180, 2019.
APA
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B. ... Chen, J. (2019). Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369. Oncology Letters, 17, 3173-3180. https://doi.org/10.3892/ol.2019.9967
MLA
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B., Sun, T., Chen, J."Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369". Oncology Letters 17.3 (2019): 3173-3180.
Chicago
Xie, W., Liu, X., Chen, Q., Ma, M., Yang, X., Gong, B., Sun, T., Chen, J."Silencing of the long non‑coding RNA GHET1 inhibits cell proliferation and migration of renal cell carcinoma through epithelial‑mesenchymal transition Corrigendum in /10.3892/ol.2019.10369". Oncology Letters 17, no. 3 (2019): 3173-3180. https://doi.org/10.3892/ol.2019.9967
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