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Article

Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells

  • Authors:
    • Zhengyong Liu
    • Yi Liu
    • Yupeng Long
    • Baohua Liu
    • Xiangfeng Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Daping Hospital, Army Medical University, Chongqing 400042, P.R. China, Department of Information, Daping Hospital, Army Medical University, Chongqing 400042, P.R. China, Department of Clinical Laboratory, Army 958 Hospital of The Chinese People's Liberation Army, Chongqing 400020, P.R. China
  • Pages: 2021-2027
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    Published online on: January 7, 2020
       https://doi.org/10.3892/ol.2020.11255
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Abstract

Multidrug resistance in cancer cells is a primary factor affecting therapeutic efficacy. Heat shock 27 kD protein 1 (HSP27) is associated with cell apoptosis and resistance to chemotherapy. However, the mechanisms underlying HSP27‑associated pathways in colon cancer cells remain unclear. Therefore, the present study used short hairpin (sh) RNA to inhibit HSP27 expression in colon cancer cells in order to investigate the effects in vitro and in vivo. Flow cytometry was used to investigate cell apoptosis and a xenograft model was employed to examine the tumorigenesis. Protein expression was measured by Western blotting. The results revealed that suppression of HSP27 expression significantly increased cell apoptosis, inhibited tumor growth and enhanced sensitivity to the anti‑cancer agents 5‑fluorouracil (5‑FU) and vincristine (VCR). shHSP27 significantly decreased the expression of notch receptor 1 and the phosphorylation level of Akt and mTOR, and enhanced the effect of 5‑FU and VCR. In conclusion, HSP27 suppression enhanced the sensitivity of colon cancer cells to 5‑FU and VCR, and increased colon cancer cell apoptosis with and without chemotherapy. Therefore, the development of novel therapeutic agents that inhibit the expression of HSP27 may offer a new treatment option for colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Liu Y, Long Y, Liu B and Wang X: Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells. Oncol Lett 19: 2021-2027, 2020.
APA
Liu, Z., Liu, Y., Long, Y., Liu, B., & Wang, X. (2020). Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells. Oncology Letters, 19, 2021-2027. https://doi.org/10.3892/ol.2020.11255
MLA
Liu, Z., Liu, Y., Long, Y., Liu, B., Wang, X."Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells". Oncology Letters 19.3 (2020): 2021-2027.
Chicago
Liu, Z., Liu, Y., Long, Y., Liu, B., Wang, X."Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells". Oncology Letters 19, no. 3 (2020): 2021-2027. https://doi.org/10.3892/ol.2020.11255
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Liu Y, Long Y, Liu B and Wang X: Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells. Oncol Lett 19: 2021-2027, 2020.
APA
Liu, Z., Liu, Y., Long, Y., Liu, B., & Wang, X. (2020). Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells. Oncology Letters, 19, 2021-2027. https://doi.org/10.3892/ol.2020.11255
MLA
Liu, Z., Liu, Y., Long, Y., Liu, B., Wang, X."Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells". Oncology Letters 19.3 (2020): 2021-2027.
Chicago
Liu, Z., Liu, Y., Long, Y., Liu, B., Wang, X."Role of HSP27 in the multidrug sensitivity and resistance of colon cancer cells". Oncology Letters 19, no. 3 (2020): 2021-2027. https://doi.org/10.3892/ol.2020.11255
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