Open Access

Role of Bcl‑2 on drug resistance in breast cancer polyploidy‑induced spindle poisons

  • Authors:
    • Bibo Yuan
    • Juan Hao
    • Qian Zhang
    • Yan Wang
    • Yu Zhu
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  • Published online on: January 7, 2020     https://doi.org/10.3892/ol.2020.11256
  • Pages: 1701-1710
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Spindle poisons are chemotherapeutic drugs used in the treatment of malignant tumors; however, numerous patients develop resistance following chemotherapy. The present study aimed to induce polyploidy in breast cancer cells using the spindle poison nocodazole to investigate the mechanism of polyploid‑induced tumor resistance. It was revealed that the spindle poison nocodazole induced apoptosis in HCC1806 cells but also induced polyploidy in MDA‑MB‑231 cells. The drug sensitivities of the polyploid MDA‑MB‑231 cells to paclitaxel, docetaxel, epirubicin, 5‑fluorouracil and oxaliplatin were lower than those of the original tumor cells; however, the polyploid MDA‑MB‑231 cells were more sensitive to etoposide than the original tumor cells. The expression of F‑box and WD repeat domain containing 7 (FBW7) was decreased, while the expression of MCL1 apoptosis regulator BCL2 family member (MCL‑1) and Bcl‑2 was increased, and caspase‑3/9 and Bax were not expressed in MDA‑MB‑231 cells. The resistance to docetaxel and etoposide was reversed, but the sensitivity of paclitaxel was not changed following Bcl‑2 silencing. The formation of polyploidy in tumors may be one of the molecular mechanisms underlying tumor resistance to spindle poisons. Expression of the Bcl‑2 family members, for example FBW7 and MCL‑1, plays a key role in apoptosis and the cell escape process that forms polyploid cells. However, Bcl‑2 silencing has different reversal effects on different anti‑tumor drugs, which requires further investigation.
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March-2020
Volume 19 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Yuan, B., Hao, J., Zhang, Q., Wang, Y., & Zhu, Y. (2020). Role of Bcl‑2 on drug resistance in breast cancer polyploidy‑induced spindle poisons. Oncology Letters, 19, 1701-1710. https://doi.org/10.3892/ol.2020.11256
MLA
Yuan, B., Hao, J., Zhang, Q., Wang, Y., Zhu, Y."Role of Bcl‑2 on drug resistance in breast cancer polyploidy‑induced spindle poisons". Oncology Letters 19.3 (2020): 1701-1710.
Chicago
Yuan, B., Hao, J., Zhang, Q., Wang, Y., Zhu, Y."Role of Bcl‑2 on drug resistance in breast cancer polyploidy‑induced spindle poisons". Oncology Letters 19, no. 3 (2020): 1701-1710. https://doi.org/10.3892/ol.2020.11256