CDCA2 acts as an oncogene and induces proliferation of clear cell renal cell carcinoma cells

Li,Fang; Li,Fang; Zhang,Huahua; Zhang,Huahua; Li,Qian; Li,Qian; Wu,Fei; Wu,Fei; Wang,Yu; Wang,Yu; Wang,Zhenzhen; Wang,Zhenzhen; Wang,Xiaofei; Wang,Xiaofei; Huang,Chen; Huang,Chen

doi:10.3892/ol.2020.11322

CDCA2 acts as an oncogene and induces proliferation of clear cell renal cell carcinoma cells

Authors:
- Fang Li
- Huahua Zhang
- Qian Li
- Fei Wu
- Yu Wang
- Zhenzhen Wang
- Xiaofei Wang
- Chen Huang
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Affiliations: Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China, Medical Research and Experimental Center, Medical College, Yan'an University, Yan'an, Shaanxi 716000, P.R. China, Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004 P.R. China
Published online on: January 21, 2020 https://doi.org/10.3892/ol.2020.11322
Pages: 2466-2474
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cell division cycle‑associated 2 (CDCA2) plays an important role in regulating chromosome structure during mitosis. It is highly expressed in oral squamous cell carcinoma, neuroblastoma and lung adenocarcinoma, and its upregulation is positively associated with tumor progression. However, the expression, biological function and underlying mechanisms of the role of CDCA2 in clear cell renal cell carcinoma (ccRCC) remain poorly understood. In the present study, CDCA2 was demonstrated to be upregulated in ccRCC tissues compared with normal kidney tissue, where higher expression was generally associated with the degree of malignancy. Small interfering RNA‑mediated knockdown of CDCA2 expression inhibited the viability and proliferation of 786‑O and CAKI‑1 cells, as measured by an MTT assay, colony formation assay and flow cytometry. Furthermore, western blot analysis suggested that CDCA2 regulates cell proliferation through the cell cycle‑associated proteins cyclin D1 and cyclin dependent kinase 4, and the apoptotic protein Bcl‑2. In conclusion, the present study indicated that CDCA2 may be an important factor in ccRCC progression and could be a potential therapeutic target in this disease.

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