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Article

C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10

  • Authors:
    • Eriko Nozaki
    • Takaaki Kobayashi
    • Hiroaki Ohnishi
    • Kouki Ohtsuka
    • Tadahiko Masaki
    • Takashi Watanabe
    • Masanori Sugiyama
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Kyorin University School of Medicine, Mitaka, Tokyo 181‑8611, Japan, Department of Medical Oncology, Kyorin University School of Medicine, Mitaka, Tokyo 181‑8611, Japan, Department of Surgery, Kyorin University School of Medicine, Mitaka, Tokyo 181‑8611, Japan
  • Pages: 2495-2501
    |
    Published online on: January 21, 2020
       https://doi.org/10.3892/ol.2020.11326
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Abstract

The chemokine CXCL10 and its receptor CXCR3 have been demonstrated to be implicated in cancer cell proliferation and metastasis. CXCR3 has three splice variants: CXCR3A, CXCR3B and CXCR3‑alt. CXCR3A and B serve multiple roles in the growth and invasiveness of a number of cancer types. However, the roles of CXCR3 isoforms in colorectal cancer (CRC) cells remain unclear. In the current study, the effects of CXCL10 and CXCR3 isoforms on proliferation and invasion of CRC cells was examined. Proliferation and invasiveness of the CRC cell line HCT116, which were transfected with CXCR3A or CXCR3B in the presence of CXCL10, were evaluated in vitro using MTT, scratch wound healing and transwell assays. MTT assay indicated that regardless of the presence or absence of CXCL10, the proliferative ability of CXCR3A‑transfected HCT116 cells was enhanced compared with blank and mock cells. Scratch wound healing and transwell assays indicated that invasiveness of CXCR3A‑transfected cells was greater compared with blank and mock cells. However, HCT116 cells transfected with CXCR3B did not exhibit changes in their proliferative or invasive ability. mRNA expression of MMP9, which is associated with signaling downstream of the CXCL10/CXCR3A pathway, was increased 4‑fold in CXCR3A‑transfected HCT116 cells compared with control cells. The results of the present study indicated that CXCL10‑enhanced proliferation and invasiveness of the CRC cell line HCT116 was likely mediated by CXCR3A, but not by CXCR3B.
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View References

1 

Brenner H, Kloor M and Pox CP: Colorectal cancer. Lancet. 383:1490–1502. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Kopetz S, Chang GJ, Overman MJ, Eng C, Sargent DJ, Larson DW, Grothey A, Vauthey JN, Nagorney DM and McWilliams RR: Improved survival in metastatic colorectal cancer is associated with adoption of hepatic resection and improved chemotherapy. J Clin Oncol. 27:3677–3683. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Kobayashi T, Masaki T, Nozaki E, Sugiyama M, Nagashima F, Furuse J, Onishi H, Watanabe T and Ohkura Y: Microarray analysis of gene expression at the tumor front of colon cancer. Anticancer Res. 35:6577–6581. 2015.PubMed/NCBI

5 

Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM and Pal S: Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: Relevance for the development of human breast cancer. Cancer Res. 66:9509–9518. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Lasagni L, Francalanci M, Annunziato F, Lazzeri E, Giannini S, Cosmi L, Sagrinati C, Mazzinghi B, Orlando C, Maggi E, et al: An alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth induced by IP-10, Mig, and I-TAC, and acts as functional receptor for platelet factor 4. J Exp Med. 197:1537–1549. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Bodnar RJ, Yates CC and Wells A: IP-10 blocks vascular endothelial growth factor-induced endothelial cell motility and tube formation via inhibition of calpain. Circ Res. 98:617–625. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Wightman SC, Uppal A, Pitroda SP, Ganai S, Burnette B, Stack M, Oshima G, Khan S, Huang X, Posner MC, et al: Oncogenic CXCL10 signalling drives metastasis development and poor clinical outcome. Br J Cancer. 113:327–335. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Bai M, Chen X and Ba YI: CXCL10/CXCR3 overexpression as a biomarker of poor prognosis in patients with stage II colorectal cancer. Mol Clin Oncol. 4:23–30. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Toiyama Y, Fujikawa H, Kawamura M, Matsushita K, Saigusa S, Tanaka K, Inoue Y, Uchida K, Mohri Y and Kusunoki M: Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer. Int J Oncol. 40:560–566. 2012.PubMed/NCBI

11 

Jiang Z, Xu Y and Cai S: CXCL10 expression and prognostic significance in stage II and III colorectal cancer. Mol Biol Rep. 37:3029–3036. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Groom JR and Luster AD: CXCR3 ligands: Redundant, collaborative and antagonistic functions. Immunol Cell Biol. 89:207–215. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Billottet C, Quemener C and Bikfalvi A: CXCR3, a double-edged sword in tumor progression and angiogenesis. Biochim Biophys Acta. 1836:287–295. 2013.PubMed/NCBI

14 

Ehlert JE, Addison CA, Burdick MD, Kunkel SL and Strieter RM: Identification and partial characterization of a variant of human CXCR3 generated by posttranscriptional exon skipping. J Immunol. 173:6234–6240. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Yang C, Zheng W and Du W: CXCR3A contributes to the invasion and metastasis of gastric cancer cells. Oncol Rep. 36:1686–1692. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Utsumi T, Suyama T, Imamura Y, Fuse M, Sakamoto S, Nihei N, Ueda T, Suzuki H, Seki N and Ichikawa T: The association of CXCR3 and renal cell carcinoma metastasis. J Urol. 192:567–574. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wu Q, Dhir R and Wells A: Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion. Mol Cancer. 11:32012. View Article : Google Scholar : PubMed/NCBI

18 

Jöhrer K, Zelle-Rieser C, Perathoner A, Moser P, Hager M, Ramoner R, Gander H, Höltl L, Bartsch G, Greil R and Thurnher M: Up-regulation of functional chemokine receptor CCR3 in human renal cell carcinoma. Clin Cancer Res. 11:2459–2465. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Jacquelot N, Enot DP, Flament C, Vimond V, Blattner C, Pitt JM, Yamazaki T, Roberti MP, Daillère R, Vétizou M, et al: Chemokine receptor patterns in lymphocytes mirror metastatic spreading in melanoma. J Clin Invest. 126:921–97. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Zhou H, Wu J, Wang T, Zhang X and Liu D: CXCL10/CXCR3 axis promotes the invasion of gastric cancer via PI3K/AKT pathway-dependent MMPs production. Biomed Pharmacother. 82:479–488. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Ma X, Norsworthy K, Kundu N, Rodgers WH, Gimotty PA, Goloubeva O, Lipsky M, Li Y, Holt D and Fulton A: CXCR3 expression is associated with poor survival in breast cancer and promotes metastasis in a murine model. Mol Cancer Ther. 8:490–498. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Kawada K, Sonoshita M, Sakashita H, Takabayashi A, Yamaoka Y, Manabe T, Inaba K, Minato N, Oshima M and Taketo MM: Pivotal role of CXCR3 in melanoma cell metastasis to lymph nodes. Cancer Res. 64:4010–4017. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Walser TC, Rifat S, Ma X, Kundu N, Ward C, Goloubeva O, Johnson MG, Medina JC, Collins TL and Fulton AM: Antagonism of CXCR3 inhibits lung metastasis in a murine model of metastatic breast cancer. Cancer Res. 66:7701–7707. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Shen D and Cao X: Potential role of CXCR3 in proliferation and invasion of prostate cancer cells. Int J Clin Exp Pathol. 8:8091–8098. 2015.PubMed/NCBI

25 

Aksoy MO, Yang Y, Ji R, Reddy PJ, Shahabuddin S, Litvin J, Rogers TJ and Kelsen SG: CXCR3 surface expression in human airway epithelial cells: Cell cycle dependence and effect on cell proliferation. Am J Physiol Lung Cell Mol Physiol. 290:L909–L918. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Ji R, Lee CM, Gonzales LW, Yang Y, Aksoy MO, Wang P, Brailoiu E, Dun N, Hurford MT and Kelsen SG: Human type II pneumocyte chemotactic responses to CXCR3 activation are mediated by splice variant A. Am J Physiol Lung Cell Mol Physiol. 294:L1187–L1196. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Zipin-Roitman A, Meshel T, Sagi-Assif O, Shalmon B, Avivi C, Pfeffer RM, Witz IP and Ben-Baruch A: CXCL10 promotes invasion-related properties in human colorectal carcinoma cells. Cancer Res. 67:3396–3405. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Nozaki E, Kobayashi T, Ohnishi H, Ohtsuka K, Masaki T, Watanabe T and Sugiyama M: C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10. Oncol Lett 19: 2495-2501, 2020.
APA
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., & Sugiyama, M. (2020). C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10. Oncology Letters, 19, 2495-2501. https://doi.org/10.3892/ol.2020.11326
MLA
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., Sugiyama, M."C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10". Oncology Letters 19.3 (2020): 2495-2501.
Chicago
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., Sugiyama, M."C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10". Oncology Letters 19, no. 3 (2020): 2495-2501. https://doi.org/10.3892/ol.2020.11326
Copy and paste a formatted citation
x
Spandidos Publications style
Nozaki E, Kobayashi T, Ohnishi H, Ohtsuka K, Masaki T, Watanabe T and Sugiyama M: C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10. Oncol Lett 19: 2495-2501, 2020.
APA
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., & Sugiyama, M. (2020). C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10. Oncology Letters, 19, 2495-2501. https://doi.org/10.3892/ol.2020.11326
MLA
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., Sugiyama, M."C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10". Oncology Letters 19.3 (2020): 2495-2501.
Chicago
Nozaki, E., Kobayashi, T., Ohnishi, H., Ohtsuka, K., Masaki, T., Watanabe, T., Sugiyama, M."C‑X‑C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C‑X‑C motif ligand 10". Oncology Letters 19, no. 3 (2020): 2495-2501. https://doi.org/10.3892/ol.2020.11326
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