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Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6

  • Authors:
    • Jieqiong Jin
    • Xuemei Chen
    • Jing Chen
    • Xin Geng
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology, Qingdao Jiaozhou Central Hospital, Qingdao, Shandong 266300, P.R. China, Department of Obstetrics and Gynecology, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan, Hubei 430060, P.R. China, Department of Gynecological Oncology, Qingdao Center Medical Group, Qingdao, Shandong 266042, P.R. China, Department of Gynaecology, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2339-2345
    |
    Published online on: January 24, 2020
       https://doi.org/10.3892/ol.2020.11346
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Abstract

The present study aimed to investigate the role of long noncoding RNA MACC1‑AS1 in cervical squamous cell carcinoma (CSCC). In the present study MACC1‑AS1 expression as analyzed using reverse transcription‑quantitative PCR. The interactions between MACC1‑AS1 and miR‑34a was analyzed via overexpression experiments. Cell cycle and proliferation analyses were performed to analyze the roles of MACC1‑AS1 in regulating cancer cell cycle progression and cell proliferation. It was observed that MACC1‑AS1 was upregulated in CSCC, and its expression levels were elevated with the increase in clinical stage. Bioinformatics analysis revealed that MACC1‑AS1 may be a sponge of miR‑34a, which can target cyclin‑dependent kinase 6 (CDK6). In CSCC cells, MACC1‑AS1 overexpression led to upregulation of CDK6, while miR‑34a overexpression had the opposite effect and reduced the effects of MACC1‑AS1 overexpression in co‑transfected cells. Cell cycle and proliferation analyses demonstrated that MACC1‑AS1 and CDK6 promoted cell cycle progression and cell proliferation. By contrast, miR‑34a had the opposite effect on cell cycle proliferation and cell proliferation, reducing the effects induced by MACC1‑AS1 overexpression. Therefore, the lncRNA MACC1‑AS1 may serve as a sponge of miR‑34a to upregulate CDK6, thereby promoting cell cycle progression and cell proliferation.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Koh WJ, Greer BE, Abu-Rustum NR, Apte SM, Campos SM, Cho KR, Chu C, Cohn D, Crispens MA, Dorigo O, et al: Cervical cancer, version 2.2015. J Natl Compr Canc Netw. 13:395–404. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Small W Jr, Bacon MA, Bajaj A, Chuang LT, Fisher BJ, Harkenrider MM, Jhingran A, Kitchener HC, Mileshkin LR, Viswanathan AN and Gaffney DK: Cervical cancer: A global health crisis. Cancer. 123:2404–2412. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Wentzensen N, Schiffman M, Palmer T and Arbyn M: Triage of HPV positive women in cervical cancer screening. J Clin Virol. 76 (Suppl 1):S49–S55. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Lowy DR: HPV vaccination to prevent cervical cancer and other HPV-associated disease: From basic science to effective interventions. J Clin Invest. 126:5–11. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Walboomers JM and Meijer CJ: Do HPV-negative cervical carcinomas exist? J Pathol. 181:253–254. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Cancer Genome Atlas Research Network; Albert Einstein College of Medicine; Analytical Biological Services; Barretos Cancer Hospital; Baylor College of Medicine; Beckman Research Institute of City of Hope; Buck Institute for Research on Aging; Canada's Michael Smith Genome Sciences Centre; Harvard Medical School; Helen F. Graham Cancer Center &Research Institute at Christiana Care Health Services, ; et al: Integrated genomic and molecular characterization of cervical cancer. Nature. 543:378–384. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Swaffer MP, Jones AW, Flynn HR, Snijders AP and Nurse P: CDK substrate phosphorylation and ordering the cell cycle. Cell. 167:1750–1761.e16. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Shen SN, Wang H and Gong BL: Expressions of miRNA29 target genes CCND2 and CDK6 in cervical cancer. Disc Clin Cases. 5:14–18. 2018. View Article : Google Scholar

10 

Sherr CJ, Beach D and Shapiro GI: Targeting CDK4 and CDK6: From discovery to therapy. Cancer Discov. 6:353–367. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Li Y, Guessous F, Zhang Y, Dipierro C, Kefas B, Johnson E, Marcinkiewicz L, Jiang J, Yang Y, Schmittgen TD, et al: MicroRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes. Cancer Res. 69:7569–7576. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Zhao Y, Liu Y, Lin L, Huang Q, He W, Zhang S, Dong S, Wen Z, Rao J, Liao W and Shi M: The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1. Mol Cancer. 17:692018. View Article : Google Scholar : PubMed/NCBI

13 

He W, Liang B, Wang C, Li S, Zhao Y, Huang Q, Liu Z, Yao Z, Wu Q, Liao W, et al: MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer. Oncogene. 38:4637–4654. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Busch A, Richter AS and Backofen R: IntaRNA: Efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions. Bioinformatics. 24:2849–2856. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Edge SB and Compton CC: The American Joint Committee on Cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Strohl AE, Mendoza G, Ghant MS, Cameron KA, Simon MA, Schink JC and Marsh EE: Barriers to prevention: Knowledge of HPV, cervical cancer, and HPV vaccinations among African American women. Am J Obstet Gynecol. 212:65 e1–e5. 2015. View Article : Google Scholar

18 

Paraskevopoulou MD and Hatzigeorgiou AG: Analyzing MiRNA- LncRNA Interactions. Methods Mol Biol. 1402:271–286. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Jalali S, Bhartiya D, Lalwani MK, Sivasubbu S and Scaria V: Systematic transcriptome wide analysis of lncRNA-miRNA interactions. PLoS One. 8:e538232013. View Article : Google Scholar : PubMed/NCBI

20 

Liu C, Kelnar K, Liu B, Chen X, Calhoun-Davis T, Li H, Patrawala L, Yan H, Jeter C, Honorio S, et al: The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44. Nat Med. 17:211–215. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Wei JS, Song YK, Durinck S, Chen QR, Cheuk AT, Tsang P, Zhang Q, Thiele CJ, Slack A, Shohet J and Khan J: The MYCN oncogene is a direct target of miR-34a. Oncogene. 27:5204–5213. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jin J, Chen X, Chen J and Geng X: Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncol Lett 19: 2339-2345, 2020.
APA
Jin, J., Chen, X., Chen, J., & Geng, X. (2020). Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncology Letters, 19, 2339-2345. https://doi.org/10.3892/ol.2020.11346
MLA
Jin, J., Chen, X., Chen, J., Geng, X."Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6". Oncology Letters 19.3 (2020): 2339-2345.
Chicago
Jin, J., Chen, X., Chen, J., Geng, X."Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6". Oncology Letters 19, no. 3 (2020): 2339-2345. https://doi.org/10.3892/ol.2020.11346
Copy and paste a formatted citation
x
Spandidos Publications style
Jin J, Chen X, Chen J and Geng X: Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncol Lett 19: 2339-2345, 2020.
APA
Jin, J., Chen, X., Chen, J., & Geng, X. (2020). Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncology Letters, 19, 2339-2345. https://doi.org/10.3892/ol.2020.11346
MLA
Jin, J., Chen, X., Chen, J., Geng, X."Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6". Oncology Letters 19.3 (2020): 2339-2345.
Chicago
Jin, J., Chen, X., Chen, J., Geng, X."Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6". Oncology Letters 19, no. 3 (2020): 2339-2345. https://doi.org/10.3892/ol.2020.11346
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