Maspin inhibits MCF‑7 cell invasion and proliferation by downregulating miR‑21 and increasing the expression of its target genes

Huang,Shao‑Xin; Fan,Wen‑Yan; Wang,Ling; Liu,Hui; Wang,Xin; Zhao,Hao; Jiang,Wen‑Bin

doi:10.3892/ol.2020.11360

Maspin inhibits MCF‑7 cell invasion and proliferation by downregulating miR‑21 and increasing the expression of its target genes

Authors:
- Shao‑Xin Huang
- Wen‑Yan Fan
- Ling Wang
- Hui Liu
- Xin Wang
- Hao Zhao
- Wen‑Bin Jiang
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Affiliations: Department of Social Science and Public Health, School of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China, Clinical Skills Center, Clinical Medical College, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China
Published online on: January 30, 2020 https://doi.org/10.3892/ol.2020.11360
Pages: 2621-2628
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Maspin has been identified as a tumor suppressor gene in breast cancer, but the underlying regulatory mechanisms remain unclear. In the present study, maspin pcDNA was transfected into MCF‑7 cells. microRNA (miR) microarray and reverse transcription‑quantitative polymerase chain reaction was used for analysis; the results demonstrated that maspin may inhibit miR‑10b, miR‑21 and miR‑451 expression in MCF‑7 cells. In addition, maspin increased the expression of certain miR‑21 target genes (phosphatase and tensin homolog, programmed cell death 4 and B‑cell lymphoma‑2), miR‑10b target gene (Homeobox D10; HOXD10) and miR‑451 target gene (multidrug resistance protein 1). Furthermore, the results of the present study revealed that decreased expression of miR‑21 suppressed the invasion and proliferation of MCF‑7 cells. Therefore, in the present study, it was hypothesized that as a tumor‑suppressor gene, the potential molecular mechanism of maspin include down‑regulating the expression of miR‑21 and increasing the expression of specific miR‑21 target genes.

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