Knockdown of long non‑coding RNA prostate cancer‑associated transcript 1 inhibits the proliferation and metastasis of tongue squamous cell carcinoma cells by upregulating p21

Yan,Ming; Zhao,Tianjiang; Fu,Lingling; Tian,Rongxiang; Li,Dongzhen; Da,Yunmeng

doi:10.3892/ol.2020.11373

Knockdown of long non‑coding RNA prostate cancer‑associated transcript 1 inhibits the proliferation and metastasis of tongue squamous cell carcinoma cells by upregulating p21

Authors:
- Ming Yan
- Tianjiang Zhao
- Lingling Fu
- Rongxiang Tian
- Dongzhen Li
- Yunmeng Da
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Affiliations: Department of Oral and Maxillofacial Surgery, Hebei Eye Hospital, Xingtai, Hebei 054000, P.R. China, Department of Stomatology, People's Hospital of Tongliang District, Chongqing 402560, P.R. China, Department of Prosthodontics, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Xingtai, Qinghe 054800, P.R. China, Department of Prosthodontics, Hebei Eye Hospital, Xingtai, Hebei 054000, P.R. China
Published online on: February 6, 2020 https://doi.org/10.3892/ol.2020.11373
Pages: 2839-2845
Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNA (lncRNA) prostate cancer‑associated transcript 1 (PCAT‑1) is a long non‑coding RNA involved in the development of various cancer types. Tongue squamous cell carcinoma (TSCC) is the most frequently diagnosed type of oral cancer worldwide. The present study aimed to explore the role of lncRNA PCAT‑1 in TSCC, and to provide a preliminary assessment of the downstream pathways involved. The expression levels of lncRNA PCAT‑1 in TSCC specimens were first assessed. Subsequently, lncRNA PCAT‑1 was knocked down in TSCC cell lines and the resulting effect on the proliferation and apoptosis, as well as the metastatic and invasive potential of TSCC cells was investigated. To assess the downstream signaling resulting from lncRNA PCAT‑1 inhibition, the expression of p21 was also detected. The results indicated that lncRNA PCAT‑1 was upregulated in clinical TSCC compared with peri‑tumor tissues. Inhibition of lncRNA PCAT‑1 resulted in reduced proliferation and apoptosis of TSCC cells, in addition to the suppression of migration and invasion. Furthermore, the expression of p21 was induced following lncRNA PCAT‑1 inhibition. Collectively, the present study demonstrated an oncogenic role for lncRNA PCAT‑1 in TSCC; inhibition of lncRNA PCAT‑1 reduced the growth, metastasis and invasion of TSCC, at least in part via the induction of p21 signaling.

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