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Article Open Access

Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells

  • Authors:
    • Xinyan Li
    • Cuicui Liu
    • Xiumei Zhao
    • Rui Wang
    • Na Gu
    • Hongsheng Shen
    • Xijing Li
    • Luyao Wang
    • Chao Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Cangzhou Medical College, Cangzhou, Hebei 061000, P.R. China, Centre for Research and Development of Anti‑tumor Drugs, Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, P.R. China, Department of Critical Care Medicine, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2909-2915
    |
    Published online on: February 14, 2020
       https://doi.org/10.3892/ol.2020.11398
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Abstract

Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells were explored. Seventy‑five cases of thyroid carcinoma and adjacent tissues were collected. The expression levels of miR‑298 and CDK6 mRNA in tissues and cells were detected by RT‑PCR. In addition, thyroid cancer cells and human normal thyroid cells Nthy‑ori3‑1 were purchased, with the former transfected with miR‑298‑mimics, miR‑298‑inhibitor, miR‑NC, si‑CDK6, si‑NC, Sh‑CDK6, Sh‑NC to build cell models. Then the expression levels of miR‑298 and CDK6 in thyroid cancer tissues and cells were detected by qRT‑PCR, and the expression of CDK6, Bax, Bcl‑2 and caspase‑3 by WB. CCK‑8 and flow cytometry were employed to detect cell proliferation and apoptosis, and dual luciferase report was adopted to determine the relationship between miR‑298 and CDK6. miR‑298 was underexpressed in thyroid cancer, and CDK6 was highly expressed in thyroid cancer. Cell experiments revealed that overexpression of miR‑298 or inhibition of CDK6 expression could suppress cell proliferation, promote apoptosis, and significantly increase the expression levels of Bax and caspase‑3 proteins, decrease Bcl‑2 protein expression, which was contrary to the biological phenotype of cells after inhibition of miR‑298 or further overexpression of CDK6. Dual luciferase report confirmed that miR‑298 was a targeting site of CDK6. miR‑298 can inhibit the proliferation of thyroid cells and promote apoptosis of thyroid cancer cells by regulating the expression of CDK6, which is expected to be a potential target for clinical application.
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1 

Zhang T, He L, Sun W, Qin Y, Zhang P and Zhang H: 1,25-Dihydroxyvitamin D3 enhances the susceptibility of anaplastic thyroid cancer cells to adriamycin-induced apoptosis by increasing the generation of reactive oxygen species. Mol Med Rep. 20:2641–2648. 2019.PubMed/NCBI

2 

Luo Y, Hao T, Zhang J, Zhang M, Sun P and Wu L: MicroRNA-592 suppresses the malignant phenotypes of thyroid cancer by regulating lncRNA NEAT1 and downregulating NOVA1. Int J Mol Med. 44:1172–1182. 2019.PubMed/NCBI

3 

Bai J, Gao Y, Du Y, Yang X and Zhang X: MicroRNA-300 inhibits the growth of hepatocellular carcinoma cells by downregulating CREPT/Wnt/β-catenin signaling. Oncol Lett. 18:3743–3753. 2019.PubMed/NCBI

4 

Zou L, Gao Z, Zeng F, Xiao J, Chen J, Feng X, Chen D, Fang Y, Cui J, Liu Y, et al: Sulfasalazine suppresses thyroid cancer cell proliferation and metastasis through T-cell originated protein kinase. Oncol Lett. 18:3517–3526. 2019.PubMed/NCBI

5 

Fuziwara CS, Saito KC and Kimura ET: Interplay of TGFβ signaling and microRNA in thyroid cell loss of differentiation and cancer progression. Arch Endocrinol Metab. 63:536–544. 2019.PubMed/NCBI

6 

Shi D, Wang H, Ding M, Yang M, Li C, Yang W and Chen L: MicroRNA-26a-5p inhibits proliferation, invasion and metastasis by repressing the expression of Wnt5a in papillary thyroid carcinoma. OncoTargets Ther. 12:6605–6616. 2019. View Article : Google Scholar

7 

Jin J, Zhang J, Xue Y, Luo L, Wang S and Tian H: miRNA-15a regulates the proliferation and apoptosis of papillary thyroid carcinoma via regulating AKT pathway. OncoTargets Ther. 12:6217–6226. 2019. View Article : Google Scholar

8 

Mo Y, He L, Lai Z, Wan Z, Chen Q, Pan S, Li L, Li D, Huang J, Xue F, et al: LINC01287/miR-298/STAT3 feedback loop regulates growth and the epithelial-to-mesenchymal transition phenotype in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 37:1492018. View Article : Google Scholar : PubMed/NCBI

9 

Zhou F, Chen J and Wang H: MicroRNA-298 inhibits malignant phenotypes of epithelial ovarian cancer by regulating the expression of EZH2. Oncol Lett. 12:3926–3932. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Meyerson M and Harlow E: Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 14:2077–2086. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Ferrari SM, Fallahi P, Galdiero MR, Ruffilli I, Elia G, Ragusa F, Paparo SR, Patrizio A, Mazzi V, Varricchi G, et al: Immune and inflammatory cells in thyroid cancer microenvironment. Int J Mol Sci. 20:202019. View Article : Google Scholar

12 

Liu W: lncRNA LINC-PINT inhibits cancer cell proliferation, invasion, and migration in osteosarcoma by downregulating miRNA-21. Cancer Biother Radiopharm. 34:258–263. 2019. View Article : Google Scholar : PubMed/NCBI

13 

Hu S, Liao Y, Zheng J, Gou L, Regmi A, Zafar MI and Chen L: In silico integration approach reveals key microRNAs and their target genes in follicular thyroid carcinoma. BioMed Res Int. 2019:27251922019. View Article : Google Scholar : PubMed/NCBI

14 

Cao N, Mu L, Yang W, Liu L, Liang L and Zhang H: MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/β-catenin signaling. Biomed Pharmacother. 106:483–490. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Jin Y-P, Hu Y-P, Wu X-S, Wu YS, Ye YY, Li HF, Liu YC, Jiang L, Liu FT, Zhang YJ, et al: miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis. 9:1822018. View Article : Google Scholar : PubMed/NCBI

16 

Lam PY, Di Tomaso E, Ng HK, Pang JC, Roussel MF and Hjelm NM: Expression of p19INK4d, CDK4, CDK6 in glioblastoma multiforme. Br J Neurosurg. 14:28–32. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Mendrzyk F, Radlwimmer B, Joos S, Kokocinski F, Benner A, Stange DE, Neben K, Fiegler H, Carter NP, Reifenberger G, et al: Genomic and protein expression profiling identifies CDK6 as novel independent prognostic marker in medulloblastoma. J Clin Oncol. 23:8853–8862. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Schwartz EI, Smilenov LB, Price MA, Osredkar T, Baker RA, Ghosh S, Shi FD, Vollmer TL, Lencinas A, Stearns DM, et al: Cell cycle activation in postmitotic neurons is essential for DNA repair. Cell cycle. 6:318–329. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Grossel MJ and Hinds PW: From cell cycle to differentiation: The role of cdk6 continues to expand. Cell Cycle. 5:266–270. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Handschick K, Beuerlein K, Jurida L, Bartkuhn M, Müller H, Soelch J, Weber A, Dittrich-Breiholz O, Schneider H, Scharfe M, et al: Cyclin-dependent kinase 6 is a chromatin-bound cofactor for NF-κB-dependent gene expression. Mol Cell. 53:193–208. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Li B, He H, Tao BB, Zhao ZY, Hu GH, Luo C, Chen JX, Ding XH, Sheng P, Dong Y, et al: Knockdown of CDK6 enhances glioma sensitivity to chemotherapy. Oncol Rep. 28:909–914. 2012.PubMed/NCBI

22 

Ma H, Chen J, Pan S, Dai J, Jin G, Hu Z, Shen H and Shu Y: Potentially functional polymorphisms in cell cycle genes and the survival of non-small cell lung cancer in a Chinese population. Lung Cancer. 73:32–37. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Liu C, Zhao X, Wang R, Gu N, Shen H, Li X, Wang L and Li C: Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncol Lett 19: 2909-2915, 2020.
APA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H. ... Li, C. (2020). Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncology Letters, 19, 2909-2915. https://doi.org/10.3892/ol.2020.11398
MLA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19.4 (2020): 2909-2915.
Chicago
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19, no. 4 (2020): 2909-2915. https://doi.org/10.3892/ol.2020.11398
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Liu C, Zhao X, Wang R, Gu N, Shen H, Li X, Wang L and Li C: Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncol Lett 19: 2909-2915, 2020.
APA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H. ... Li, C. (2020). Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncology Letters, 19, 2909-2915. https://doi.org/10.3892/ol.2020.11398
MLA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19.4 (2020): 2909-2915.
Chicago
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19, no. 4 (2020): 2909-2915. https://doi.org/10.3892/ol.2020.11398
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