Open Access

Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells

  • Authors:
    • Xinyan Li
    • Cuicui Liu
    • Xiumei Zhao
    • Rui Wang
    • Na Gu
    • Hongsheng Shen
    • Xijing Li
    • Luyao Wang
    • Chao Li
  • View Affiliations

  • Published online on: February 14, 2020     https://doi.org/10.3892/ol.2020.11398
  • Pages: 2909-2915
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells were explored. Seventy‑five cases of thyroid carcinoma and adjacent tissues were collected. The expression levels of miR‑298 and CDK6 mRNA in tissues and cells were detected by RT‑PCR. In addition, thyroid cancer cells and human normal thyroid cells Nthy‑ori3‑1 were purchased, with the former transfected with miR‑298‑mimics, miR‑298‑inhibitor, miR‑NC, si‑CDK6, si‑NC, Sh‑CDK6, Sh‑NC to build cell models. Then the expression levels of miR‑298 and CDK6 in thyroid cancer tissues and cells were detected by qRT‑PCR, and the expression of CDK6, Bax, Bcl‑2 and caspase‑3 by WB. CCK‑8 and flow cytometry were employed to detect cell proliferation and apoptosis, and dual luciferase report was adopted to determine the relationship between miR‑298 and CDK6. miR‑298 was underexpressed in thyroid cancer, and CDK6 was highly expressed in thyroid cancer. Cell experiments revealed that overexpression of miR‑298 or inhibition of CDK6 expression could suppress cell proliferation, promote apoptosis, and significantly increase the expression levels of Bax and caspase‑3 proteins, decrease Bcl‑2 protein expression, which was contrary to the biological phenotype of cells after inhibition of miR‑298 or further overexpression of CDK6. Dual luciferase report confirmed that miR‑298 was a targeting site of CDK6. miR‑298 can inhibit the proliferation of thyroid cells and promote apoptosis of thyroid cancer cells by regulating the expression of CDK6, which is expected to be a potential target for clinical application.
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April-2020
Volume 19 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li X, Liu C, Zhao X, Wang R, Gu N, Shen H, Li X, Wang L and Li C: Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncol Lett 19: 2909-2915, 2020
APA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H. ... Li, C. (2020). Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells. Oncology Letters, 19, 2909-2915. https://doi.org/10.3892/ol.2020.11398
MLA
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19.4 (2020): 2909-2915.
Chicago
Li, X., Liu, C., Zhao, X., Wang, R., Gu, N., Shen, H., Li, X., Wang, L., Li, C."Effects of CDK6 regulated by miR‑298 on proliferation and apoptosis of thyroid cancer cells". Oncology Letters 19, no. 4 (2020): 2909-2915. https://doi.org/10.3892/ol.2020.11398