MicroRNA‑212 suppresses cell proliferation in nasopharyngeal carcinoma by targeting ELF3

Kang,Yaojie; Cui,Yanfei; Tan,Ming

doi:10.3892/ol.2020.11401

MicroRNA‑212 suppresses cell proliferation in nasopharyngeal carcinoma by targeting ELF3

Authors:
- Yaojie Kang
- Yanfei Cui
- Ming Tan
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Affiliations: Department of Otolaryngology-Head and Neck Surgery, Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China, Department of Oncology, Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China
Published online on: February 17, 2020 https://doi.org/10.3892/ol.2020.11401
Pages: 2902-2908
Copyright: © Kang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy of the head and neck that is prevalent in China. The present study investigated the molecular mechanisms of microRNA‑212 (miR‑212) and E74‑like factor 3 (ELF3) in NPC cell lines and tissues. Using reverse transcription‑quantitative PCR, the present study identified that miR‑212 expression was downregulated in NPC cell lines and tissues. Furthermore, an elevated expression level of miR‑212 was revealed to inhibit NPC cell proliferation, as determined using a cell counting kit‑8 assay in vitro. ELF3 was identified as a direct target of miR‑212 in NPC cells by a luciferase reporter assay. Additionally, the expression levels of miR‑212 and ELF3 were negatively correlated in NPC tissues. The expression levels of ELF3 and miR‑212 were associated with metastasis and TNM stage in patients with NPC. In summary, the present study indicated that miR‑212 was downregulated in NPC and suppressed cell proliferation. This suggested that the miR‑212/ELF3 axis may serve as a novel target for the diagnosis and treatment of NPC.

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1	Han P, Wang X, Liang F, Liu Y, Qiu X, Xu Y, Chen R, Yu S and Huang X: Osteoradionecrosis of the skull base in nasopharyngeal carcinoma: Incidence and risk factors. Int J Radiat Oncol Biol Phys. 102:552–555. 2018. View Article : Google Scholar : PubMed/NCBI
2	Lin K, Qiu F, Chen S, He X, Peng S and Chen H: Lack of association between the distribution of ABO blood groups and nasopharyngeal carcinoma in a population of Southern China. J Cancer Res Ther. 14:785–788. 2018. View Article : Google Scholar : PubMed/NCBI
3	Nakanishi Y, Wakisaka N, Kondo S, Endo K, Sugimoto H, Hatano M, Ueno T, Ishikawa K and Yoshizaki T: Progression of understanding for the role of Epstein-Barr virus and management of nasopharyngeal carcinoma. Cancer Metastasis Rev. 36:435–447. 2017. View Article : Google Scholar : PubMed/NCBI
4	Lin Z, Yang Z, He B, Wang D, Gao X, Tam SY and Wu VWC: Pattern of radiation-induced thyroid gland changes in nasopharyngeal carcinoma patients in 48 months after radiotherapy. PLoS One. 13:e02003102018. View Article : Google Scholar : PubMed/NCBI
5	Hezaveh K, Kloetgen A, Bernhart SH, Mahapatra KD, Lenze D, Richter J, Haake A, Bergmann AK, Brors B, Burkhardt B, et al: Alterations of microRNA and microRNA-regulated messenger RNA expression in germinal center B-cell lymphomas determined by integrative sequencing analysis. Haematologica. 101:1380–1389. 2016. View Article : Google Scholar : PubMed/NCBI
6	Dallaire A and Simard MJ: The implication of microRNAs and endo-siRNAs in animal germline and early development. Dev Biol. 416:18–25. 2016. View Article : Google Scholar : PubMed/NCBI
7	Chai C, Wu H, Wang B, Eisenstat DD and Leng RP: MicroRNA-498 promotes proliferation and migration by targeting the tumor suppressor PTEN in breast cancer cells. Carcinogenesis. 39:1185–1196. 2018. View Article : Google Scholar : PubMed/NCBI
8	Gorbea C, Mosbruger T and Cazalla D: A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis. Nature. 550:275–279. 2017. View Article : Google Scholar : PubMed/NCBI
9	Chen D, Hu S, Wu Z, Liu J and Li S: The role of MiR-132 in regulating neural stem cell proliferation, differentiation and neuronal maturation. Cell Physiol Biochem. 47:2319–2330. 2018. View Article : Google Scholar : PubMed/NCBI
10	Wang LX, Kang ZP, Yang ZC, Ma RX, Tan Y, Peng XB, Dai RZ, Li J, Yu Y and Xu M: MicroRNA-135a inhibits nasopharyngeal carcinoma cell proliferation through targeting interleukin-17. Cell Physiol Biochem. 46:2232–2238. 2018. View Article : Google Scholar : PubMed/NCBI
11	Bao L, You B, Shi S, Shan Y, Zhang Q, Yue H, Zhang J, Zhang W, Shi Y, Liu Y, et al: Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10. Oncogene. 37:2873–2889. 2018. View Article : Google Scholar : PubMed/NCBI
12	Jiang N, Jiang X, Chen Z, Song X, Wu L, Zong D, Song D, Yin L, Wang D, Chen C, et al: MiR-203a-3p suppresses cell proliferation and metastasis through inhibiting LASP1 in nasopharyngeal carcinoma. J Exp Clin Cancer Res. 36:1382017. View Article : Google Scholar : PubMed/NCBI
13	Tang T, Huan L, Zhang S, Zhou H, Gu L, Chen X and Zhang L: MicroRNA-212 functions as a tumor-suppressor in human non-small cell lung cancer by targeting SOX4. Oncol Rep. 38:2243–2250. 2017. View Article : Google Scholar : PubMed/NCBI
14	Li D, Li Z, Xiong J, Gong B, Zhang G, Cao C, Jie Z, Liu Y, Cao Y, Yan Y, et al: MicroRNA-212 functions as an epigenetic-silenced tumor suppressor involving in tumor metastasis and invasion of gastric cancer through down-regulating PXN expression. Am J Cancer Res. 5:2980–2997. 2015.PubMed/NCBI
15	Jiang C, Wang H, Zhou L, Jiang T, Xu Y and Xia L: MicroRNA-212 inhibits the metastasis of nasopharyngeal carcinoma by targeting SOX4. Oncol Rep. 38:82–88. 2017. View Article : Google Scholar : PubMed/NCBI
16	Ma C, Nong K, Wu B, Dong B, Bai Y, Zhu H, Wang W, Huang X, Yuan Z and Ai K: miR-212 promotes pancreatic cancer cell growth and invasion by targeting the hedgehog signaling pathway receptor patched-1. J Exp Clin Cancer Res. 33:542014. View Article : Google Scholar : PubMed/NCBI
17	Oliver JR, Kushwah R and Hu J: Multiple roles of the epithelium-specific ETS transcription factor, ESE-1, in development and disease. Lab Invest. 92:320–330. 2012. View Article : Google Scholar : PubMed/NCBI
18	Wang JL, Chen ZF, Chen HM, Wang MY, Kong X, Wang YC, Sun TT, Hong J, Zou W, Xu J and Fang JY: Elf3 drives β-catenin transactivation and associates with poor prognosis in colorectal cancer. Cell Death Dis. 5:e12632014. View Article : Google Scholar : PubMed/NCBI
19	Zheng L, Xu M, Xu J, Wu K, Fang Q, Liang Y, Zhou S, Cen D, Ji L, Han W and Cai X: ELF3 promotes epithelial-mesenchymal transition by protecting ZEB1 from miR-141-3p-mediated silencing in hepatocellular carcinoma. Cell Death Dis. 9:3872018. View Article : Google Scholar : PubMed/NCBI
20	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
21	Li L and Luo Z: Dysregulated miR-27a-3p promotes nasopharyngeal carcinoma cell proliferation and migration by targeting Mapk10. Oncol Rep. 37:2679–2687. 2017. View Article : Google Scholar : PubMed/NCBI
22	Zhang Y and Xu Z: miR-93 enhances cell proliferation by directly targeting CDKN1A in nasopharyngeal carcinoma. Oncol Lett. 15:1723–1727. 2018.PubMed/NCBI
23	Li LN, Xiao T, Yi HM, Zheng Z, Qu JQ, Huang W, Ye X, Yi H, Lu SS, Li XH and Xiao ZQ: MiR-125b increases nasopharyngeal carcinoma radioresistance by targeting A20/NF-κB signaling pathway. Mol Cancer Ther. 16:2094–2106. 2017. View Article : Google Scholar : PubMed/NCBI
24	Cheng JZ, Chen JJ, Wang ZG and Yu D: MicroRNA-185 inhibits cell proliferation while promoting apoptosis and autophagy through negative regulation of TGF-β1/mTOR axis and HOXC6 in nasopharyngeal carcinoma. Cancer Biomark. 23:107–123. 2018. View Article : Google Scholar : PubMed/NCBI
25	Jia P, Wei G, Zhou C, Gao Q, Wu Y, Sun X and Li X: Upregulation of MiR-212 inhibits migration and tumorigenicity and inactivates Wnt/β-catenin signaling in human hepatocellular carcinoma. Technol Cancer Res Treat. 17:15330346187652212018. View Article : Google Scholar : PubMed/NCBI
26	Hu B, Jin X and Wang J: MicroRNA-212 targets mitogen-activated protein kinase 1 to inhibit proliferation and invasion of prostate cancer cells. Oncol Res. 26:1093–1102. 2018. View Article : Google Scholar : PubMed/NCBI
27	Song Y, Liu Y and Chen X: MiR-212 attenuates MPP⁺-induced neuronal damage by targeting KLF4 in SH-SY5Y cells. Yonsei Med J. 59:416–424. 2018. View Article : Google Scholar : PubMed/NCBI
28	Liu Y, Zhang XL, Li XF, Tang YC and Zhao X: miR-212-3p reduced proliferation, and promoted apoptosis of fibroblast-like synoviocytes via down-regulating SOX5 in rheumatoid arthritis. Eur Rev Med Pharmacol Sci. 22:461–471. 2018.PubMed/NCBI