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Article Open Access

Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway

  • Authors:
    • Huasheng Liang
  • View Affiliations / Copyright

    Affiliations: Institute of Endocrine and Metabolic Diseases, Beihai People's Hospital, Beihai, Guangxi Zhuang Autonomous Region 536000, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3215-3222
    |
    Published online on: February 19, 2020
       https://doi.org/10.3892/ol.2020.11413
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Abstract

The aim of the present study was to determine the mechanism by which advanced glycation end products (AGEs) induce proliferation, invasion and epithelial‑mesenchymal transition (EMT) of human colon cancer SW480 cells. SW480 cells were divided into groups as follows: i) Control; ii) cells treated with AGEs alone; and iii) cells treated with AGEs combined with LY294002. Proliferation, cell cycle progression, apoptosis, invasion and migration of SW480 cells were assessed using an MTT assay, flow cytometry, Transwell assays and a wound healing assay, respectively. The protein expression levels of PI3K, AKT and epithelial cadherin (E‑cadherin) were examined by western blot analysis in SW480 cells treated with various concentrations of AGEs. Proliferation, invasion and migration were enhanced, cell cycle progression was increased and apoptosis was decreased in SW480 cells treated with AGEs compared with the control. The PI3K inhibitor, LY294002, reversed the effects of AGEs. Western blot analysis data demonstrated that AGEs increased the protein expression levels of PI3K and AKT, and decreased the expression of E‑cadherin. The results suggested that AGEs exert a positive effect on the proliferation, invasion and EMT in SW480 cells through the PI3K/AKT signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liang H: Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway. Oncol Lett 19: 3215-3222, 2020.
APA
Liang, H. (2020). Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway. Oncology Letters, 19, 3215-3222. https://doi.org/10.3892/ol.2020.11413
MLA
Liang, H."Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway". Oncology Letters 19.4 (2020): 3215-3222.
Chicago
Liang, H."Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway". Oncology Letters 19, no. 4 (2020): 3215-3222. https://doi.org/10.3892/ol.2020.11413
Copy and paste a formatted citation
x
Spandidos Publications style
Liang H: Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway. Oncol Lett 19: 3215-3222, 2020.
APA
Liang, H. (2020). Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway. Oncology Letters, 19, 3215-3222. https://doi.org/10.3892/ol.2020.11413
MLA
Liang, H."Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway". Oncology Letters 19.4 (2020): 3215-3222.
Chicago
Liang, H."Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway". Oncology Letters 19, no. 4 (2020): 3215-3222. https://doi.org/10.3892/ol.2020.11413
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