[Corrigendum] P130cas is required for TGF‑β1‑mediated epithelial‑mesenchymal transition in lung cancer

Figure 1.

(A) Immunoblotting was conducted to detect the expression levels of p130cas, p-p130cas, p38, p-p38, Smad2 and p-Smad2. The expression levels of p130cas-protein and p-p130cas were reduced markedly in the A549-p130cas-RNAi cells compared with the negative control cells. TGF-β1 treatment (7.5 ng/ml) for 12 h or 36 h markedly increased the p130cas expression levels in the A549 cells. The expression levels of total-p38 and p-p38 were reduced significantly in the A549-p130cas-RNAi cells compared with the A549-negative control cells. The total-p38 expression level was significantly upregulated in the A549-p130cas-RNAi cells following TGF-β1 treatment; however, the p-p38 expression level in the A549-p130cas-RNAi cells was consistently significantly lower than in the A549-negative control cells prior to or following TGF-β1 treatment. TGF-β1 remarkably increased the expression levels of p-Smad2 in the A549 cells following 1 h of treatment. However, p130cas-knockdown had no impact on the expression of total-Smad2 or p-Smad2 compared with the control cells. (B) Quantification of immunoblotting was conducted with Quantity One software. The analyses of the bands of the different proteins were reference against GAPDH. Mean ± SEM values were calculated in order to establish any statistically significant differences. TGF-β1, transforming growth factor-β1; RNAi, RNA interference; cas, Crk-associated substrate.