Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis

  • Authors:
    • Yi Zhang
    • Liangjia Bi
    • Zheng Hu
    • Wenwu Cao
    • Deshu Zhuang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin, Heilongjiang 150080, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3223-3228
    |
    Published online on: February 21, 2020
       https://doi.org/10.3892/ol.2020.11419
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

It has been found that >90% of oral cancer patients suffer from squamous cell carcinoma (SCC). The 5‑year survival rate of SCC is ~50%, despite the availability of different treatments. Sonodynamic therapy (SDT) has been developed as a novel therapy for cancer, resisting bacterial infection and inhibiting atherosclerotic plaque progression. The present study investigated the efficacy of hematoporphyrin monomethyl ether (HMME)‑mediated SDT on the A‑253 epidermoid cancer cell line. The cytotoxicity of HMME and the survival rate of cells following SDT were examined by the MTT assay. Apoptosis and necrosis of cells were detected using flow cytometry with Annexin V and propidium iodide (PI) staining, and fluorescence microscopy with Hoechst 33258 and PI staining. Intracellular reactive oxygen species (ROS) and Ca2+ levels were measured using a fluorescence microscope based on 2',7'‑dichlorofluorescein diacetate and fluo‑3/acetoxymethylester, respectively. Results of the MTT assay demonstrated that a lower concentration (<10 µg/ml) of HMME had no significant effect on the A‑253 cells, but SDT combined with ultrasonic treatment for 1 min and 10 µg/ml HMME decreased the cell survival rate by 27%. Flow cytometry analysis revealed that A‑253 cells in the SDT group had a higher rate of late apoptosis compared with the control group. Furthermore, fluorescence quantitation of apoptotic A‑253 cells demonstrated that the percentages of apoptotic cells were increased in the ultrasound and SDT group compared with those in the control group. In the present study, the ROS level in the SDT group was elevated compared with that in the control group. The Ca2+ levels were increased to 181.2 and 268.7% in the ultrasound and SDT groups, respectively, relative to the control group. Taken together, the findings of the present study demonstrated that HMME‑SDT significantly induces the apoptosis of A‑253 cells together with intracellular ROS generation and Ca2+ overload. Thus, HMME‑SDT may be a promising treatment option for patients with SCC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Pérez-Sayáns M, Suárez-Peñaranda JM, Gayoso-Diz P, Barros-Angueira F, Gándara-Rey JM and García-García A: The role of p21Waf1/CIP1 as a Cip/Kip type cell-cycle regulator in oral squamous cell carcinoma (Review). Med Oral Patol Oral Cir Bucal. 18:e219–e225. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Somasundaram RT, Kaur J, Leong I, MacMillan C, Witterick IJ, Walfish PG and Ralhan R: Subcellular differential expression of Ep-ICD in oral dysplasia and cancer is associated with disease progression and prognosis. BMC Cancer. 16:4862016. View Article : Google Scholar : PubMed/NCBI

4 

Taghavi N and Yazdi I: Prognostic factors of survival rate in oral squamous cell carcinoma: Clinical, histologic, genetic and molecular concepts. Arch Iran Med. 18:314–319. 2015.PubMed/NCBI

5 

Kerker FA, Adler W, Brunner K, Moest T, Wurm MC, Nkenke E, Neukam FW and von Wilmowsky C: Anatomical locations in the oral cavity where surgical resections of oral squamous cell carcinomas are associated with a close or positive margin-a retrospective study. Clin Oral Investig. 22:1625–1630. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Su X, Wang P, Wang X, Cao B, Li L and Liu Q: Apoptosis of U937 cells induced by hematoporphyrin monomethyl ether-mediated sonodynamic action. Cancer Biother Radiopharm. 28:207–217. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Zhuang D, Hou C, Bi L, Han J, Hao Y, Cao W and Zhou Q: Sonodynamic effects of hematoporphyrin monomethyl ether on staphylococcus aureus in vitro. FEMS Microbiol Lett. 361:174–180. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Yang Y, Liu Y, Chen X, Gong J, Huang Z, Wang W, Shi Y, Wang Y, Yao J, Shen Z, et al: 5-aminolevulinic acid-mediated sonodynamic therapy alleviates atherosclerosis via enhancing efferocytosis and facilitating a shift in the Th1/Th2 balance toward Th2 polarization. Cell Physiol Biochem. 47:83–96. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Bilmin K, Kujawska T, Secomski W, Nowicki A and Grieb P: 5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro. Folia Neuropathol. 54:234–240. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Hao D, Song Y, Che Z and Liu Q: Calcium overload and in vitro apoptosis of the C6 glioma cells mediated by sonodynamic therapy (hematoporphyrin monomethyl ether and ultrasound). Cell Biochem Biophys. 70:1445–1452. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Su X, Wang P, Yang S, Zhang K, Liu Q and Wang X: Sonodynamic therapy induces the interplay between apoptosis and autophagy in K562 cells through ROS. Int J Biochem Cell Biol. 60:82–92. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Li Y, Zhou Q, Deng Z, Pan M, Liu X, Wu J, Yan F and Zheng H: IR-780 Dye as a sonosensitizer for sonodynamic therapy of breast tumor. Sci Rep. 6:259682016. View Article : Google Scholar : PubMed/NCBI

13 

Bullon P, Cordero MD, Quiles JL, Morillo JM, del Carmen Ramirez-Tortosa M and Battino M: Mitochondrial dysfunction promoted by Porphyromonas gingivalis lipopolysaccharide as a possible link between cardiovascular disease and periodontitis. Free Radic Biol Med. 50:1336–1343. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Wang X, Jia Y, Wang P, Liu Q and Zheng H: Current status and future perspectives of sonodynamic therapy in glioma treatment. Ultrason Sonochem. 37:592–599. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Sun X, Xu H, Shen J, Guo S, Shi S, Dan J, Tian F and Tian Y and Tian Y: Real-time detection of intracellular reactive oxygen species and mitochondrial membrane potential in THP-1 macrophages during ultrasonic irradiation for optimal sonodynamic therapy. Ultrason Sonochem. 22:7–14. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Li Y, Wang P, Zhao P, Zhu S, Wang X and Liu Q: Apoptosis induced by sonodynamic treatment by protoporphyrin IX on MDA-MB-231 cells. Ultrasonics. 52:490–496. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Liu ZR, Chen SQ, Zou YW, Wu XY, Li HY, Wang XQ, Shi Y and Niu HX: Hypochlorite modified albumins promote cell death in the tubule interstitium in rats via mitochondrial damage in obstructive nephropathy and the protective effects of antioxidant peptides. Free Radic Res. 52:616–628. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Li N, Sun M, Wang Y, Lv Y, Hu Z, Cao W, Zheng J and Jiao X: Effect of cell cycle phase on the sensitivity of SAS cells to sonodynamic therapy using low-intensity ultrasound combined with 5-aminolevulinic acid in vitro. Mol Med Rep. 12:3177–3183. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Lv Y, Zheng J, Zhou Q, Jia L, Wang C, Liu N, Zhao H, Ji H, Li B and Cao W: Antiproliferative and apoptosis-inducing effect of exo-protoporphyrin IX based sonodynamic therapy on human oral squamous cell carcinoma. Sci Rep. 7:409672017. View Article : Google Scholar : PubMed/NCBI

20 

Sisto M, Lisi S, D'Amore M, Mitolo V and Scagliusi P: Anti-Ro and anti-La autoantibodies induce TNF-alpha production by human salivary gland cells: An in vitro study. Reumatismo. 59:221–226. 2007.PubMed/NCBI

21 

Li W, Fei JF, Yang Q, Li BL, Lin C, Yue Q and Meng QG: Acute toxic effects of sonodynamic therapy on hypertrophic scar fibroblasts of rabbit ears. Genet Mol Res. 14:4203–4214. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Xie R, Xu T, Zhu J, Wei X, Zhu W, Li L, Wang Y, Han Y, Zhou J and Bai Y: The combination of glycolytic inhibitor 2-deoxyglucose and microbubbles increases the effect of 5-aminolevulinic acid-sonodynamic therapy in liver cancer cells. Ultrasound Med Biol. 43:2640–2650. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Xiong W, Wang P, Hu J, Jia Y, Wu L, Chen X, Liu Q and Wang X: A new sensitizer DVDMS combined with multiple focused ultrasound treatments: An effective antitumor strategy. Sci Rep. 5:174852015. View Article : Google Scholar : PubMed/NCBI

24 

Sun H, Ge W, Gao X, Wang S, Jiang S, Hu Y, Yu M and Hu S: Apoptosis-promoting effects of hematoporphyrin monomethyl ether-sonodynamic therapy (HMME-SDT) on endometrial cancer. PLoS One. 10:e01379802015. View Article : Google Scholar : PubMed/NCBI

25 

Wang F, Gao Q, Guo S, Cheng J, Sun X, Li Q, Wang T, Zhang Z, Cao W and Tian Y: The sonodynamic effect of curcumin on THP-1 cell-derived macrophages. BioMed Res Int. 2013:7372642013.PubMed/NCBI

26 

Qin P, Han T, Yu ACH and Xu L: Mechanistic understanding the bioeffects of ultrasound-driven microbubbles to enhance macromolecule delivery. J Control Release. 272:169–181. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Li J, Yue W, Huang Z, Chen ZQ, Zhan Q, Ren FB, Liu JY and Fu SB: Calcium overload induces C6 rat glioma cell apoptosis in sonodynamic therapy. Int J Radiat Biol. 87:1061–1066. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Tian J, Gan Y, Pan C, Zhang M, Wang X, Tang X and Peng X: Nerol-induced apoptosis associated with the generation of ROS and Ca2+ overload in saprotrophic fungus Aspergillus flavus. Appl Microbiol Biotechnol. 102:6659–6672. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Tran TA, Roger S, Le Guennec JY, Tranquart F and Bouakaz A: Effect of ultrasound-activated microbubbles on the cell electrophysiological properties. Ultrasound Med Biol. 33:158–163. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Lentacker I, De Cock I, Deckers R, De Smedt SC and Moonen CT: Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms. Adv Drug Deliv Rev. 72:49–64. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Bi L, Hu Z, Cao W and Zhuang D: Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis. Oncol Lett 19: 3223-3228, 2020.
APA
Zhang, Y., Bi, L., Hu, Z., Cao, W., & Zhuang, D. (2020). Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis. Oncology Letters, 19, 3223-3228. https://doi.org/10.3892/ol.2020.11419
MLA
Zhang, Y., Bi, L., Hu, Z., Cao, W., Zhuang, D."Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis". Oncology Letters 19.4 (2020): 3223-3228.
Chicago
Zhang, Y., Bi, L., Hu, Z., Cao, W., Zhuang, D."Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis". Oncology Letters 19, no. 4 (2020): 3223-3228. https://doi.org/10.3892/ol.2020.11419
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Bi L, Hu Z, Cao W and Zhuang D: Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis. Oncol Lett 19: 3223-3228, 2020.
APA
Zhang, Y., Bi, L., Hu, Z., Cao, W., & Zhuang, D. (2020). Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis. Oncology Letters, 19, 3223-3228. https://doi.org/10.3892/ol.2020.11419
MLA
Zhang, Y., Bi, L., Hu, Z., Cao, W., Zhuang, D."Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis". Oncology Letters 19.4 (2020): 3223-3228.
Chicago
Zhang, Y., Bi, L., Hu, Z., Cao, W., Zhuang, D."Hematoporphyrin monomethyl ether‑mediated sonodynamic therapy induces A‑253 cell apoptosis". Oncology Letters 19, no. 4 (2020): 3223-3228. https://doi.org/10.3892/ol.2020.11419
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team