Open Access

Differential behavior of NF‑κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo

  • Authors:
    • Telma Pariente‑Pérez
    • Francisco Aguilar‑Alonso
    • José Dolores Solano
    • Chabetty Vargas‑Olvera
    • Patricia Curiel‑Muñiz
    • Carmen Adriana Mendoza‑Rodríguez
    • Daniela Tenorio‑Hernández
    • María Elena Ibarra‑Rubio
  • View Affiliations

  • Published online on: March 3, 2020     https://doi.org/10.3892/ol.2020.11436
  • Pages: 3153-3164
  • Copyright: © Pariente‑Pérez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Renal cell carcinoma (RCC) is the most common type of cancer of the adult kidney. It is generally asymptomatic even at advanced stages, so opportune diagnosis is rare, making it almost impossible to study this cancer at its early stages. RCC tumors induced by ferric nitrilotriacetate (FeNTA) in rats histologically correspond to the human clear cell RCC subtype (ccRCC) and the exposure to this carcinogen during either one or two months leads to different early stages of neoplastic development. High levels of nuclear factor kappa B (NF‑κB) and epidermal growth factor receptor (EGFR) as well as low levels of NF‑κB inhibitor alpha (IκBα) are frequent in human RCC, but their status in FeNTA‑induced tumors and their evolution along renal carcinogenesis is unclear. On this basis, in the present study NF‑κB, IκBα and EGFR behavior was analyzed at different stages of the experimental renal carcinogenesis model. Similar to patients with RCC, neoplastic tissue showed high levels of p65, one of the predominant subunits of NF‑κB in ccRCC and of EGFR (protein and mRNA), as well as a decrease in the levels of NF‑κB's main inhibitor, IκBα, resulting in a classic oncogenic combination. Conversely, different responses were observed at early stages of carcinogenesis. After one month of FeNTA‑exposure, NF‑κB activity and EGFR levels augmented; but unexpectedly, IκBα also did. While after two months, NF‑κB activity diminished, but EGFR and IκBα levels remained elevated. In conclusion, FeNTA‑induced tumors and RCC human neoplasms are analogues regarding to the classic NF‑κB, IκBα and EGFR behavior, and distinctive non‑conventional combination of changes is developed at each early stage studied. The results obtained suggest that the dysregulation of the analyzed molecules could be related to different signaling pathways and therefore, to particular effects depending on the phase of the carcinogenic process.
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April-2020
Volume 19 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Pariente‑Pérez T, Aguilar‑Alonso F, Solano JD, Vargas‑Olvera C, Curiel‑Muñiz P, Mendoza‑Rodríguez CA, Tenorio‑Hernández D and Ibarra‑Rubio ME: Differential behavior of NF‑κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo. Oncol Lett 19: 3153-3164, 2020
APA
Pariente‑Pérez, T., Aguilar‑Alonso, F., Solano, J.D., Vargas‑Olvera, C., Curiel‑Muñiz, P., Mendoza‑Rodríguez, C.A. ... Ibarra‑Rubio, M.E. (2020). Differential behavior of NF‑κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo. Oncology Letters, 19, 3153-3164. https://doi.org/10.3892/ol.2020.11436
MLA
Pariente‑Pérez, T., Aguilar‑Alonso, F., Solano, J. D., Vargas‑Olvera, C., Curiel‑Muñiz, P., Mendoza‑Rodríguez, C. A., Tenorio‑Hernández, D., Ibarra‑Rubio, M. E."Differential behavior of NF‑κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo". Oncology Letters 19.4 (2020): 3153-3164.
Chicago
Pariente‑Pérez, T., Aguilar‑Alonso, F., Solano, J. D., Vargas‑Olvera, C., Curiel‑Muñiz, P., Mendoza‑Rodríguez, C. A., Tenorio‑Hernández, D., Ibarra‑Rubio, M. E."Differential behavior of NF‑κB, IκBα and EGFR during the renal carcinogenic process in an experimental model in vivo". Oncology Letters 19, no. 4 (2020): 3153-3164. https://doi.org/10.3892/ol.2020.11436