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Article Open Access

Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells

  • Authors:
    • Hongwei Yao
    • Min Fan
    • Xiaozhou He
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3269-3277
    |
    Published online on: March 3, 2020
       https://doi.org/10.3892/ol.2020.11442
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Abstract

As a polyphenolic compound, resveratrol (Res) is widely distributed in a variety of plants. Previous studies have demonstrated that Res can inhibit various different types of tumor growth. However, its role in renal cell carcinoma (RCC) remains largely unknown. The present study first demonstrated that Res inhibited cell viability and induced apoptosis in RCC 786‑O cells. Further experiments revealed that Res damaged the mitochondria and activated caspase 3. In contrast, Z‑VAD‑FMK, a pan‑caspase inhibitor, suppressed Res‑induced apoptosis. Reactive oxygen species (ROS) were involved in the process of Res‑induced apoptosis, and antioxidant N‑acetyl cysteine could significantly attenuate this. Furthermore, Res activated c‑Jun N‑terminal kinase via ROS to induce autophagy, whereas inhibition of autophagy with chloroquine or Beclin 1 small interfering RNA aggravated Res‑induced apoptosis, indicating that autophagy served as a pro‑survival mechanism to protect 786‑O cells from Res‑induced apoptosis. Therefore, a combination of Res and autophagy inhibitors could enhance the inhibitory effect of Res on RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yao H, Fan M and He X: Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells. Oncol Lett 19: 3269-3277, 2020.
APA
Yao, H., Fan, M., & He, X. (2020). Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells. Oncology Letters, 19, 3269-3277. https://doi.org/10.3892/ol.2020.11442
MLA
Yao, H., Fan, M., He, X."Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells". Oncology Letters 19.4 (2020): 3269-3277.
Chicago
Yao, H., Fan, M., He, X."Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells". Oncology Letters 19, no. 4 (2020): 3269-3277. https://doi.org/10.3892/ol.2020.11442
Copy and paste a formatted citation
x
Spandidos Publications style
Yao H, Fan M and He X: Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells. Oncol Lett 19: 3269-3277, 2020.
APA
Yao, H., Fan, M., & He, X. (2020). Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells. Oncology Letters, 19, 3269-3277. https://doi.org/10.3892/ol.2020.11442
MLA
Yao, H., Fan, M., He, X."Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells". Oncology Letters 19.4 (2020): 3269-3277.
Chicago
Yao, H., Fan, M., He, X."Autophagy suppresses resveratrol‑induced apoptosis in renal cell carcinoma 786‑O cells". Oncology Letters 19, no. 4 (2020): 3269-3277. https://doi.org/10.3892/ol.2020.11442
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