Open Access

Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study

  • Authors:
    • Milos Lazarevic
    • Maja Milosevic
    • Drago Jelovac
    • Sanja Milenkovic
    • Zvezdana Tepavcevic
    • Federica Baldan
    • Tijana Suboticki
    • Bosko Toljic
    • Dijana Trisic
    • Miroslav Dragovic
    • Giuseppe Damante
    • Jelena Milasin
  • View Affiliations

  • Published online on: March 29, 2020     https://doi.org/10.3892/ol.2020.11494
  • Pages: 3743-3750
  • Copyright: © Lazarevic et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT‑associated markers, including vimentin, α‑smooth muscle actin, SLUG and SNAIL. In addition, other EMT‑associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription‑quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.
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June-2020
Volume 19 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lazarevic M, Milosevic M, Jelovac D, Milenkovic S, Tepavcevic Z, Baldan F, Suboticki T, Toljic B, Trisic D, Dragovic M, Dragovic M, et al: Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncol Lett 19: 3743-3750, 2020
APA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F. ... Milasin, J. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncology Letters, 19, 3743-3750. https://doi.org/10.3892/ol.2020.11494
MLA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19.6 (2020): 3743-3750.
Chicago
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19, no. 6 (2020): 3743-3750. https://doi.org/10.3892/ol.2020.11494