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Article Open Access

Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study

  • Authors:
    • Milos Lazarevic
    • Maja Milosevic
    • Drago Jelovac
    • Sanja Milenkovic
    • Zvezdana Tepavcevic
    • Federica Baldan
    • Tijana Suboticki
    • Bosko Toljic
    • Dijana Trisic
    • Miroslav Dragovic
    • Giuseppe Damante
    • Jelena Milasin
  • View Affiliations / Copyright

    Affiliations: Department of Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade 11000, Serbia, Clinic for Maxillofacial Surgery, School of Dental Medicine, University of Belgrade, Belgrade 11000, Serbia, Department of Clinical Pathology, Clinical‑Hospital Center ‘Zemun’, Faculty of Medicine, University of Belgrade, Belgrade 11000, Serbia, Department of Pathology, School of Dental Medicine, University of Belgrade, Belgrade 11000, Serbia, Department of Medical Area, University of Udine, I‑33100 Udine, Italy, Department of Molecular Oncology, Institute for Medical Research, University of Belgrade, Belgrade 11000, Serbia
    Copyright: © Lazarevic et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3743-3750
    |
    Published online on: March 29, 2020
       https://doi.org/10.3892/ol.2020.11494
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Abstract

Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT‑associated markers, including vimentin, α‑smooth muscle actin, SLUG and SNAIL. In addition, other EMT‑associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription‑quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.
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1 

Maeng YI, Kim KH, Kim JY, Lee SJ, Sung WJ, Lee CK, Park JB and Park KK: Transcription factors related to epithelial mesenchymal transition in tumor center and margin in invasive lung adenocarcinoma. Int J Clin Exp Pathol. 7:4095–4103. 2014.PubMed/NCBI

2 

Acloque H, Adams MS, Fishwick K, Bronner-Fraser M and Nieto MA: Epithelial-mesenchymal transitions: The importance of changing cell state in development and disease. J Clin Invest. 119:1438–1449. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Lim J and Thiery JP: Epithelial-mesenchymal transitions: Insights from development. Development. 139:3471–3486. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2:442–454. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Moreno-Bueno G, Portillo F and Cano A: Transcriptional regulation of cell polarity in EMT and cancer. Oncogene. 27:6958–6969. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Peinado H, Olmeda D and Cano A: Snail, ZEB and bHLH factors in tumour progression: An alliance against the epithelial phenotype? Nat Rev Cancer. 7:415–428. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Misra A, Pandey C, Sze SK and Thanabalu T: Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT). PLoS One. 7:497662012. View Article : Google Scholar

9 

Liang L, Zeng M, Pan H, Liu H and He Y: Nicotinamide N-methyltransferase promotes epithelial-mesenchymal transition in gastric cancer cells by activating transforming growth factor-β1 expression. Oncol Lett. 15:4592–4598. 2018.PubMed/NCBI

10 

Krisanaprakornkit S and Iamaroon A: Epithelial-mesenchymal transition in oral squamous cell carcinoma. ISRN Oncol. 2012:6814692012.PubMed/NCBI

11 

Zhang Z, Dong Z, Lauxen IS, Filho MS and Nor JE: Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res. 74:2869–2881. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Lazarevic M, Milosevic M, Trisic D, Toljic B, Simonovic J, Nikolic N, Mikovic N, Jelovac D, Petrovic M, Vukadinovic M and Milasin J: Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. J BUON. 23:1686–1692. 2018.PubMed/NCBI

13 

Nassar A, Radhakrishnan A, Cabrero IA, Cotsonis GA and Cohen C: Intratumoral heterogeneity of immunohistochemical marker expression in breast carcinoma: A tissue microarray-based study. Appl Immunohistochem Mol Morphol. 18:433–441. 2010.PubMed/NCBI

14 

Brabletz T, Jung A, Reu S, Porzner M, Hlubek F, Kunz-Schughart LA, Knuechel R and Kirchner T: Variable beta-catenin expression in colorectal cancers indicates tumor progression driven by the tumor environment. Proc Natl Acad Sci. 98:10356–10361. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Zacharias M, Brcic L, Eidenhammer S and Popper H: Bulk tumour cell migration in lung carcinomas might be more common than epithelial-mesenchymal transition and be differently regulated. BMC Cancer. 18:7172018. View Article : Google Scholar : PubMed/NCBI

16 

Eljabo N, Nikolic N, Carkic J, Jelovac D, Lazarevic M, Tanic N and Milasin J: Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. Int J Oral Maxillofac Surg. 47:976–982. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Jelovac DB, Tepavčević Z, Nikolić N, Ilić B, Eljabo N, Popović B, Čarkić J, Konstantinović V, Vukadinović M, Miličić B and Milašin J: The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. Int J Oral Maxillofac Surg. 45:700–705. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Mitra A, Mishra L and Li S: Technologies for deriving primary tumor cells for use in personalized cancer therapy. Trends Biotechnol. 31:347–354. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Jones JC: Reduction of contamination of epithelial cultures by fibroblasts. CSH Protoc. pdb.prot4478. 2008.

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using r, eal-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Trivanović D, Kocić J, Mojsilović S, Krstić A, Ilić V, Djordjević IO, Santibanez JF, Jovcić G, Terzić M and Bugarski D: Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly. Srp Arh Celok Lek. 141:178–186. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Wong K, Rubenthiran U and Jothy S: Motility of colon cancer cells: Modulation by CD44 isoform expression. Exp Mol Pathol. 75:124–130. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Feller LL, Khammissa RR, Kramer BB and Lemmer JJ: Oral squamous cell carcinoma in relation to field precancerisation: Pathobiology. Cancer Cell Int. 13:31–39. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Zaravinos A: The regulatory role of MicroRNAs in EMT and cancer. J Oncol. 2015:8658162015. View Article : Google Scholar : PubMed/NCBI

25 

Kurihara K, Isobe T, Yamamoto G, Tanaka Y, Katakura A and Tachikawa T: Expression of BMI1 and ZEB1 in epithelial-mesenchymal transition of tongue squamous cell carcinoma. Oncol Rep. 34:771–778. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Qin X, Yan M, Li R, Ye D, Zhang J, Xu Q, Feng Y, Sun Q, Jiang C and Chen W: Identification and characterization of a highly metastatic epithelial cancer cell line from rat tongue cancer. Arch Oral Biol. 95:58–67. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Ota I, Masui T, Kurihara M, Yook JI, Mikami S, Kimura T, Shimada K, Konishi N, Yane K, Yamanaka T and Kitahara T: Snail-induced EMT promotes cancer stem cell-like properties in head and neck cancer cells. Oncol Rep. 35:261–266. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Van Marck VL and Bracke ME: Epithelial-mesenchymal transitions in human cancer. Madame Curie Bioscience Database. (Landes Bioscience, Austin). 2000.

29 

Vega S, Morales AV, Ocaña OH, Valdés F, Fabregat I and Nieto MA: Snail blocks the cell cycle and confers resistance to cell death. Genes Dev. 18:1131–1143. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Mejlvang J, Kriajevska M, Vandewalle C, Chernova T, Sayan AE, Berx G, Mellon JK and Tulchinsky E: direct repression of cyclin D1 by sip1 attenuates cell cycle progression in cells undergoing an epithelial mesenchymal transition. Mol Biol Cell. 18:4615–4624. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, Reinhard F, Zhang CC, Shipitsin M, et al: The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell. 133:704–715. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Martin TA, Goyal A, Watkins G and Jiang WG: Expression of the transcription factors snail, slug, and twist and their clinical significance in human breast cancer. Ann Surg Oncol. 12:488–496. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Alkatout I, Wiedermann M, Bauer M, Wenners A, Jonat W and Klapper W: Transcription factors associated with epithelial-mesenchymal transition and cancer stem cells in the tumor centre and margin of invasive breast cancer. Exp Mol Pathol. 94:168–173. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Kim MY, Oskarsson T, Acharyya S, Nguyen DX, Zhang XH, Norton L and Massagué J: Tumor self-seeding by circulating cancer cells. Cell. 139:1315–1326. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lazarevic M, Milosevic M, Jelovac D, Milenkovic S, Tepavcevic Z, Baldan F, Suboticki T, Toljic B, Trisic D, Dragovic M, Dragovic M, et al: Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncol Lett 19: 3743-3750, 2020.
APA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F. ... Milasin, J. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncology Letters, 19, 3743-3750. https://doi.org/10.3892/ol.2020.11494
MLA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19.6 (2020): 3743-3750.
Chicago
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19, no. 6 (2020): 3743-3750. https://doi.org/10.3892/ol.2020.11494
Copy and paste a formatted citation
x
Spandidos Publications style
Lazarevic M, Milosevic M, Jelovac D, Milenkovic S, Tepavcevic Z, Baldan F, Suboticki T, Toljic B, Trisic D, Dragovic M, Dragovic M, et al: Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncol Lett 19: 3743-3750, 2020.
APA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F. ... Milasin, J. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study. Oncology Letters, 19, 3743-3750. https://doi.org/10.3892/ol.2020.11494
MLA
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19.6 (2020): 3743-3750.
Chicago
Lazarevic, M., Milosevic, M., Jelovac, D., Milenkovic, S., Tepavcevic, Z., Baldan, F., Suboticki, T., Toljic, B., Trisic, D., Dragovic, M., Damante, G., Milasin, J."Marked epithelial to mesenchymal transition in surgical margins of oral cancer‑an in vitro study". Oncology Letters 19, no. 6 (2020): 3743-3750. https://doi.org/10.3892/ol.2020.11494
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