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MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway

  • Authors:
    • Xinli Zhang
    • Jumei Xu
    • Hongchang Zhang
    • Jilan Sun
    • Na Li
    • Xianyu Huang
  • View Affiliations / Copyright

    Affiliations: Medical Records Management Office, Qingdao West Coast New Area Central Hospital, Qingdao, Shandong 266555, P.R. China, Department of Hepatobiliary Gastrointestinal Surgery, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Infectious Diseases, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Sterilization Supply Room, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Radiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Radiotherapy Center, Qingdao Central Hospital, Qingdao University, Qingdao, Shandong 266042, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3923-3930
    |
    Published online on: April 6, 2020
       https://doi.org/10.3892/ol.2020.11516
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Abstract

Recently, a number of microRNAs (miRNAs) have been reported to play different roles in human cancers, including colorectal cancer (CRC). However, the specific role of miR‑758 has not been clarified in CRC. Therefore, the aim of the present study was to explore the role of miR‑758 in CRC. RT‑qPCR and western blot analysis were used to quantify the expression of miR‑758 and genes. The function of miR‑758 in CRC was investigated using Transwell, CCK‑8 and luciferase reporter assays. According to the results, the downregulation of miR‑758 expression was associated with aggressive behavior and poor prognosis in CRC patients. miR‑758 was shown to restrain the cell viability and metastasis in CRC. In addition, it was confirmed that miR‑758 directly targets PAX6 and inhibits CRC progression through targeting PAX6. The results also revealed that miR‑758 blocked EMT and PI3K/AKT pathway in CRC. In conclusion, miR‑758 acts as a tumor suppressor in CRC by downregulating PAX6.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Primavesi F, Stattner S, Jager T, Gobel G, Presl J, Tomanova K, Buchner S, Maglione M, Resch T, Hutter J, et al: Progressive oncological surgery is associated with increased curative resection rates and improved survival in metastatic colorectal cancer. Cancers (Basel). 11:E2182019. View Article : Google Scholar : PubMed/NCBI

3 

Kopetz S, Chang GJ, Overman MJ, Eng C, Sargent DJ, Larson DW, Grothey A, Vauthey JN, Nagorney DM and McWilliams RR: Improved survival in metastatic colorectal cancer is associated with adoption of hepatic resection and improved chemotherapy. J Clin Oncol. 27:3677–3683. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Liang Z, Li X, Liu S, Li C, Wang X and Xing J: MiR-141-3p inhibits cell proliferation, migration and invasion by targeting TRAF5 in colorectal cancer. Biochem Biophys Res Commun. 514:699–705. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Li X, Zhang X, Zhang Q and Lin R: miR-182 contributes to cell proliferation, invasion and tumor growth in colorectal cancer by targeting DAB2IP. Int J Biochem Cell Biol. 111:27–36. 2019. View Article : Google Scholar : PubMed/NCBI

7 

Zhou GH, Lu YY, Xie JL, Gao ZK, Wu XB, Yao WS and Gu WG: Overexpression of miR-758 inhibited proliferation, migration, invasion, and promoted apoptosis of non-small cell lung cancer cells by negatively regulating HMGB. Biosci Rep. 39:BSR201808552019. View Article : Google Scholar : PubMed/NCBI

8 

Meng X, Zhao Y, Wang J, Gao Z, Geng Q and Liu X: Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer. Exp Ther Med. 14:2789–2794. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Elso C, Lu X, Weisner PA, Thompson HL, Skinner A, Carver E and Stubbs L: A reciprocal translocation dissects roles of Pax6 alternative promoters and upstream regulatory elements in the development of pancreas, brain, and eye. Genesis. 51:630–646. 2013.PubMed/NCBI

10 

Li L, Li B, Zhang H, Bai S, Wang Y, Zhao B and Jonas JB: Lentiviral vector-mediated PAX6 overexpression promotes growth and inhibits apoptosis of human retinoblastoma cells. Invest Ophthalmol Vis Sci. 52:8393–8400. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Zong X, Yang H, Yu Y, Zou D, Ling Z, He X and Meng X: Possible role of Pax-6 in promoting breast cancer cell proliferation and tumorigenesis. BMB Rep. 44:595–600. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Needhamsen M, White RB, Giles KM, Dunlop SA and Thomas MG: Regulation of Human PAX6 Expression by miR-7. Evol Bioinform Online. 10:107–113. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Slattery ML, Mullany LE, Sakoda LC, Wolff RK, Stevens JR, Samowitz WS and Herrick JS: The PI3K/AKT signaling pathway: Associations of miRNAs with dysregulated gene expression in colorectal cancer. Mol Carcinog. 57:243–261. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Song Y, Zhao Y, Ding X and Wang X: microRNA-532 suppresses the PI3K/Akt signaling pathway to inhibit colorectal cancer progression by directly targeting IGF-1R. Am J Cancer Res. 8:435–449. 2018.PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Wang L, Xu M, Lu P and Zhou F: microRNA-769 is downregulated in colorectal cancer and inhibits cancer progression by directly targeting cyclin-dependent kinase 1. Onco Targets Ther. 11:9013–9025. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Hu X, Li Y, Kong D, Hu L, Liu D and Wu J: Long noncoding RNA CASC9 promotes LIN7A expression via miR-758-3p to facilitate the malignancy of ovarian cancer. J Cell Physiol. 234:10800–10808. 2019. View Article : Google Scholar : PubMed/NCBI

18 

Guo J, Zhang Z, Pan L and Zhou Y: Identification of miR-758-3p as potential modulator of CBX5 expression in gastric cancer. Technol Cancer Res Treat. 17:15330338188160612018. View Article : Google Scholar : PubMed/NCBI

19 

Wu X, Chen B, Shi H, Zhou J, Zhou F, Cao J and Sun X: miR-758-3p suppresses human bladder cancer cell proliferation, migration and invasion by targeting NOTCH2. Exp Ther Med. 17:4273–4278. 2019.PubMed/NCBI

20 

Jiang D, Cho W, Li Z, Xu X, Qu Y, Jiang Z, Guo L and Xu G: MiR-758-3p suppresses proliferation, migration and invasion of hepatocellular carcinoma cells via targeting MDM2 and mTOR. Biomed Pharmacother. 96:535–544. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Liu J, Jiang J, Hui X, Wang W, Fang D and Ding L: MiR-758-5p suppresses glioblastoma proliferation, migration and invasion by targeting ZBTB20. Cell Physiol Biochem. 48:2074–2083. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Wang S and Jiang M: The long non-coding RNA-DANCR exerts oncogenic functions in non-small cell lung cancer via miR-758-3p. Biomed Pharmacother. 103:94–100. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Zhang H, Zheng J, Lin J, Chen J, Yu Z, Chen C and Liu T: miR-758 mediates oxLDL-dependent vascular endothelial cell damage by suppressing the succinate receptor SUCNR1. Gene. 663:1–8. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Li BR, Xia LQ, Liu J, Liao LL, Zhang Y, Deng M, Zhong HJ, Feng TT, He PP and Ouyang XP: miR-758-5p regulates cholesterol uptake via targeting the CD36 3′UTR. Biochem Biophys Res Commun. 494:384–389. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Li X, Yang L, Shuai T, Piao T and Wang R: MiR-433 inhibits retinoblastoma malignancy by suppressing Notch1 and PAX6 expression. Biomed Pharmacother. 82:247–255. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Zou Q, Yi W, Huang J, Fu F, Chen G and Zhong D: MicroRNA-375 targets PAX6 and inhibits the viability, migration and invasion of human breast cancer MCF-7 cells. Exp Ther Med. 14:1198–1204. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Li Y, Li Y, Liu Y, Xie P, Li F and Li G: PAX6, a novel target of microRNA-7, promotes cellular proliferation and invasion in human colorectal cancer cells. Dig Dis Sci. 59:598–606. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Cheng Q, Cao H, Chen Z, Ma Z, Wan X, Peng R and Jiang B: PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells. Mol Med Rep. 10:399–404. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Yuan F, Liu J, Pang H, Tian Y, Yuan K, Li Y, Wang J, Bian S, Zheng Y, Dong D, et al: MicroRNA-365 suppressed cell proliferation and migration via targeting PAX6 in glioblastoma. Am J Transl Res. 11:361–369. 2019.PubMed/NCBI

30 

Meng Y, Zou Q, Liu T, Cai X, Huang Y and Pan J: microRNA-335 inhibits proliferation, cell-cycle progression, colony formation, and invasion via targeting PAX6 in breast cancer cells. Mol Med Rep. 11:379–385. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Wang J, Wang X, Wu G, Hou D and Hu Q: MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. FEBS Lett. 587:1779–1186. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Xu J, Zhang H, Sun J, Li N and Huang X: MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway. Oncol Lett 19: 3923-3930, 2020.
APA
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., & Huang, X. (2020). MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway. Oncology Letters, 19, 3923-3930. https://doi.org/10.3892/ol.2020.11516
MLA
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., Huang, X."MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway". Oncology Letters 19.6 (2020): 3923-3930.
Chicago
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., Huang, X."MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway". Oncology Letters 19, no. 6 (2020): 3923-3930. https://doi.org/10.3892/ol.2020.11516
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Xu J, Zhang H, Sun J, Li N and Huang X: MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway. Oncol Lett 19: 3923-3930, 2020.
APA
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., & Huang, X. (2020). MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway. Oncology Letters, 19, 3923-3930. https://doi.org/10.3892/ol.2020.11516
MLA
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., Huang, X."MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway". Oncology Letters 19.6 (2020): 3923-3930.
Chicago
Zhang, X., Xu, J., Zhang, H., Sun, J., Li, N., Huang, X."MicroRNA‑758 acts as a tumor inhibitor in colorectal cancer through targeting PAX6 and regulating PI3K/AKT pathway". Oncology Letters 19, no. 6 (2020): 3923-3930. https://doi.org/10.3892/ol.2020.11516
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