Open Access

Ubiquitin‑specific peptidase 17 promotes cisplatin resistance via PI3K/AKT activation in non‑small cell lung cancer

  • Authors:
    • Shengchao Zhang
    • Zhenglang Xu
    • Jun Yuan
    • Hao Chen
  • View Affiliations

  • Published online on: April 23, 2020     https://doi.org/10.3892/ol.2020.11568
  • Pages: 67-74
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The suppression of ubiquitin-specific peptidase 17 (USP17) has previously been found to result in reduced tumorigenesis and invasion of non‑small cell lung cancer (NSCLC) cells. However, the functions and underlying mechanisms of USP17 in NSCLC progression remain unclear. In the present study, cisplatin treatment was found to upregulate USP17 expression in a dose‑dependent manner. Furthermore, USP17‑overexpressing (USP17‑OE) NSCLC A549 and H1299 cells were generated for mechanistic studies. The results from the Cell Counting Kit‑8 assay revealed increased cell proliferation in USP17‑OE cells compared with that of control cells. Moreover, the viability of USP17‑OE cells was significantly higher than that of the control cells, when treated with cisplatin. The results of the biochemical studies demonstrated enhanced PI3K and AKT phosphorylation in USP17‑OE NSCLC cells, whereas USP17‑knockdown decreased these levels of phosphorylation. By contrast, an AKT inhibitor abolished the USP17‑mediated enhancement of proliferation. Moreover, suppression of USP17 or the combination of the AKT inhibitor and cisplatin significantly reduced cell viability. Overall, the results of the present study suggest that PI3K/AKT activation is the underlying mechanism of USP17‑mediated cisplatin resistance in NSCLC.

Related Articles

Journal Cover

July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Zhang, S., Xu, Z., Yuan, J., & Chen, H. (2020). Ubiquitin‑specific peptidase 17 promotes cisplatin resistance via PI3K/AKT activation in non‑small cell lung cancer. Oncology Letters, 20, 67-74. https://doi.org/10.3892/ol.2020.11568
MLA
Zhang, S., Xu, Z., Yuan, J., Chen, H."Ubiquitin‑specific peptidase 17 promotes cisplatin resistance via PI3K/AKT activation in non‑small cell lung cancer". Oncology Letters 20.1 (2020): 67-74.
Chicago
Zhang, S., Xu, Z., Yuan, J., Chen, H."Ubiquitin‑specific peptidase 17 promotes cisplatin resistance via PI3K/AKT activation in non‑small cell lung cancer". Oncology Letters 20, no. 1 (2020): 67-74. https://doi.org/10.3892/ol.2020.11568