Open Access

Vitamin D3 mediates miR‑15a‑5p inhibition of liver cancer cell proliferation via targeting E2F3

  • Authors:
    • Yulong Li
    • Qiang Lin
    • Su'e Chang
    • Rong Zhang
    • Jingjie Wang
  • View Affiliations

  • Published online on: April 24, 2020     https://doi.org/10.3892/ol.2020.11572
  • Pages: 292-298
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Vitamin D3 has been demonstrated to suppress the development and progression of liver cancer, but the mechanism is unclear. The effects of vitamin D3 and microRNA (miR)‑15a‑5p on liver cancer cells were investigated in the present study using MTT and colony formation assays, flow cytometry, western blotting and reverse transcription‑quantitative PCR. A dual‑luciferase reporter assay was performed to determine whether E2F transcription factor 3 (E2F3) was a target of miR‑15a‑5p. The effects of silencing the E2F3 gene expression in liver cancer cells were investigated using a small interfering RNA. Vitamin D3 suppressed liver cancer cell proliferation, induced apoptosis and increased miR‑15a‑5p expression. Treatment with the miR‑15a‑5p mimics significantly suppressed liver cancer cell proliferation compared with that of the controls. Bioinformatics analysis and a dual‑luciferase reporter assay demonstrated that E2F3 was a target of miR‑15a‑5p and that silencing E2F3 inhibited liver cancer cell proliferation. Therefore, Vitamin D3 suppressed cell proliferation by miR‑15a‑5p‑mediated silencing of E2F3 gene expression. These findings suggested a role for vitamin D3 and E2F3 targeting as potential novel liver cancer therapies.

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July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Li, Y., Lin, Q., Chang, S., Zhang, R., & Wang, J. (2020). Vitamin D3 mediates miR‑15a‑5p inhibition of liver cancer cell proliferation via targeting E2F3. Oncology Letters, 20, 292-298. https://doi.org/10.3892/ol.2020.11572
MLA
Li, Y., Lin, Q., Chang, S., Zhang, R., Wang, J."Vitamin D3 mediates miR‑15a‑5p inhibition of liver cancer cell proliferation via targeting E2F3". Oncology Letters 20.1 (2020): 292-298.
Chicago
Li, Y., Lin, Q., Chang, S., Zhang, R., Wang, J."Vitamin D3 mediates miR‑15a‑5p inhibition of liver cancer cell proliferation via targeting E2F3". Oncology Letters 20, no. 1 (2020): 292-298. https://doi.org/10.3892/ol.2020.11572