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Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs

  • Authors:
    • Shasha Liu
    • Qiuyue Du
    • Yang Rao
    • Caiyan Liu
    • Pengpeng Qu
  • View Affiliations / Copyright

    Affiliations: Emergency Department, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, P.R. China, Pathology Department, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, P.R. China, Department of Gynecological Oncology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, P.R. China, State Key Laboratory of Medicinal Chemical Biology, NanKai University, Tianjin 300071, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 685-692
    |
    Published online on: May 18, 2020
       https://doi.org/10.3892/ol.2020.11639
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Abstract

Ovarian cancer has a high incidence rate and mortality in gynaecologic malignancies. Epithelial ovarian cancer (EOC) accounts for >95% of ovarian cancer cases. Most of the patients with EOC are difficult to diagnose in early stage. The aim of the present study was to compare the long non‑coding (lnc)RNA expression profiles of five ovarian cancer cell lines (IGROV1, A2780, SKOV3, ES2, and Hey) and an ovarian epithelial cell line (IOSE80) in order to identify differentially expressed lncRNAs and their associated microRNAs (miRNAs). The expression profiles of lncRNAs and mRNAs in these cell lines were determined by microarray gene analysis and reverse transcription‑quantitative PCR. lncRNA neuropeptides B and W receptor 1‑2 (NPBWR1‑2) overexpression was induced in the SKOV3 cell line. Cell viability, proliferation, migration, invasion and apoptosis were evaluated using MTT, colony‑formation, Transwell and flow cytometry assays, respectively. The microarray results indicated that several lncRNAs were differentially expressed in the five ovarian cancer cell lines compared with the normal ovarian epithelial cell line. Compared with IOSE80, lncRNA NPBWR1‑2 was downregulated by more than two‑fold in all five ovarian cancer cell lines. Moreover, NPBWR1‑2 overexpression in the SKOV3 cell line decreased cell viability, inhibited proliferation, migration and invasion, and promoted apoptosis compared with the control cells. A total of 20 miRNAs, which are involved in tumorigenesis and development, were predicted to be associated with NPBWR1‑2 by bioinformatics analysis. The results of the present study suggest that lncRNA NPBWR1‑2 affects the occurrence and development of ovarian cancer via multiple miRNAs, providing a theoretical basis for the development of novel clinical treatments.
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Spandidos Publications style
Liu S, Du Q, Rao Y, Liu C and Qu P: Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs. Oncol Lett 20: 685-692, 2020.
APA
Liu, S., Du, Q., Rao, Y., Liu, C., & Qu, P. (2020). Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs. Oncology Letters, 20, 685-692. https://doi.org/10.3892/ol.2020.11639
MLA
Liu, S., Du, Q., Rao, Y., Liu, C., Qu, P."Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs". Oncology Letters 20.1 (2020): 685-692.
Chicago
Liu, S., Du, Q., Rao, Y., Liu, C., Qu, P."Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs". Oncology Letters 20, no. 1 (2020): 685-692. https://doi.org/10.3892/ol.2020.11639
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Du Q, Rao Y, Liu C and Qu P: Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs. Oncol Lett 20: 685-692, 2020.
APA
Liu, S., Du, Q., Rao, Y., Liu, C., & Qu, P. (2020). Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs. Oncology Letters, 20, 685-692. https://doi.org/10.3892/ol.2020.11639
MLA
Liu, S., Du, Q., Rao, Y., Liu, C., Qu, P."Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs". Oncology Letters 20.1 (2020): 685-692.
Chicago
Liu, S., Du, Q., Rao, Y., Liu, C., Qu, P."Long non‑coding RNA NPBWR1‑2 affects the development of ovarian cancer via multiple microRNAs". Oncology Letters 20, no. 1 (2020): 685-692. https://doi.org/10.3892/ol.2020.11639
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