Open Access

Mutations in the BCR‑ABL1 kinase domain in patients with chronic myeloid leukaemia treated with TKIs or at diagnosis

  • Authors:
    • Jingjing Liu
    • Haiping Yang
    • Xiuwen Xu
    • Shujuan Yi
    • Li Meng
  • View Affiliations

  • Published online on: May 20, 2020     https://doi.org/10.3892/ol.2020.11650
  • Pages: 1071-1076
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to analyse the incidence of mutations in the BCR‑ABL1 kinase region in patients with newly diagnosed or treated chronic myeloid leukaemia (CML), and the association between mutations clinicopathological characteristics. Samples were collected for mutation analysis from patients who exhibited tyrosine kinase inhibitor resistance following treatment or were in the accelerated or blast phase at diagnosis. The mutations in the breakpoint cluster region (BCR)‑ABL proto‑oncogene 1 (ABL1) kinase domain were evaluated using conventional sequencing or ultra‑deep sequencing (UDS) of peripheral blood samples. Sanger sequencing and UDS of the cDNA region corresponding to the BCR‑ABL1 kinase domain was performed. χ2 test was used to assess the association of categorical variables between the mutated and non‑mutated groups. In addition, the Kaplan‑Meier method was applied to generate the survival curves. Sequencing detected 28 different mutations in 54 of the 175 (30.86%) patients with CML. A total of 14 (8.0%) patients presented with the T315I mutation, accounting for the largest proportion in the mutated group. Eight patients (4.6%) presented with more than one mutation, three (37.5%) of whom harboured T315I coexisting with other mutations, and for nine (5.1%) patients, the results differed between conventional sequencing and UDS, with the mutations being missed by conventional sequencing. The results form this study suggested that programing mutation analysis in patients with chronic myeloid leukaemia timely may guide the choice of TKIs.

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August-2020
Volume 20 Issue 2

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Spandidos Publications style
Liu J, Yang H, Xu X, Yi S and Meng L: Mutations in the BCR‑ABL1 kinase domain in patients with chronic myeloid leukaemia treated with TKIs or at diagnosis. Oncol Lett 20: 1071-1076, 2020
APA
Liu, J., Yang, H., Xu, X., Yi, S., & Meng, L. (2020). Mutations in the BCR‑ABL1 kinase domain in patients with chronic myeloid leukaemia treated with TKIs or at diagnosis. Oncology Letters, 20, 1071-1076. https://doi.org/10.3892/ol.2020.11650
MLA
Liu, J., Yang, H., Xu, X., Yi, S., Meng, L."Mutations in the BCR‑ABL1 kinase domain in patients with chronic myeloid leukaemia treated with TKIs or at diagnosis". Oncology Letters 20.2 (2020): 1071-1076.
Chicago
Liu, J., Yang, H., Xu, X., Yi, S., Meng, L."Mutations in the BCR‑ABL1 kinase domain in patients with chronic myeloid leukaemia treated with TKIs or at diagnosis". Oncology Letters 20, no. 2 (2020): 1071-1076. https://doi.org/10.3892/ol.2020.11650