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Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells

  • Authors:
    • Zhenzhen Ren
    • Jie Geng
    • Chao Xiong
    • Xuebing Li
    • Yuqing Li
    • Jin Li
    • Hongchun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Medical Laboratory, The Second Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1163-1170
    |
    Published online on: May 21, 2020
       https://doi.org/10.3892/ol.2020.11654
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Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignancy worldwide. The disease has a poor prognosis and a low 5-year survival rate. Therefore, it is necessary to identify new strategies to optimize the treatment of ESCC. Vaccinia‑related kinase (VRK1) and barrier‑to‑autointegration factor 1 (BANF1) are overexpressed in ESCC. In the present study, the roles of VRK1 and BANF1 were explored in the development of ESCC. In the present study, the effects of small interfering (si)RNA‑induced downregulation of VRK1 on BANF1 expression were investigated as well as the effects on proliferative and migratory activity of ESCC cells. Western blot analysis indicated that the protein expression levels of BANF1 were decreased following siRNA depletion of VRK1. Furthermore, the depletion of VRK1 expression inhibited the proliferation and migration of ESCC cell lines, and flow cytometry analysis indicated that the depletion of VRK1 triggered cell cycle arrest mainly in the S phase. These results suggested that VRK1 and BANF1 may have pivotal roles in the progression of ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Ren Z, Geng J, Xiong C, Li X, Li Y, Li J and Liu H: Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells. Oncol Lett 20: 1163-1170, 2020.
APA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., & Liu, H. (2020). Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells. Oncology Letters, 20, 1163-1170. https://doi.org/10.3892/ol.2020.11654
MLA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20.2 (2020): 1163-1170.
Chicago
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20, no. 2 (2020): 1163-1170. https://doi.org/10.3892/ol.2020.11654
Copy and paste a formatted citation
x
Spandidos Publications style
Ren Z, Geng J, Xiong C, Li X, Li Y, Li J and Liu H: Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells. Oncol Lett 20: 1163-1170, 2020.
APA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., & Liu, H. (2020). Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells. Oncology Letters, 20, 1163-1170. https://doi.org/10.3892/ol.2020.11654
MLA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20.2 (2020): 1163-1170.
Chicago
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20, no. 2 (2020): 1163-1170. https://doi.org/10.3892/ol.2020.11654
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