Open Access

Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells

  • Authors:
    • Zhenzhen Ren
    • Jie Geng
    • Chao Xiong
    • Xuebing Li
    • Yuqing Li
    • Jin Li
    • Hongchun Liu
  • View Affiliations

  • Published online on: May 21, 2020     https://doi.org/10.3892/ol.2020.11654
  • Pages: 1163-1170
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignancy worldwide. The disease has a poor prognosis and a low 5-year survival rate. Therefore, it is necessary to identify new strategies to optimize the treatment of ESCC. Vaccinia‑related kinase (VRK1) and barrier‑to‑autointegration factor 1 (BANF1) are overexpressed in ESCC. In the present study, the roles of VRK1 and BANF1 were explored in the development of ESCC. In the present study, the effects of small interfering (si)RNA‑induced downregulation of VRK1 on BANF1 expression were investigated as well as the effects on proliferative and migratory activity of ESCC cells. Western blot analysis indicated that the protein expression levels of BANF1 were decreased following siRNA depletion of VRK1. Furthermore, the depletion of VRK1 expression inhibited the proliferation and migration of ESCC cell lines, and flow cytometry analysis indicated that the depletion of VRK1 triggered cell cycle arrest mainly in the S phase. These results suggested that VRK1 and BANF1 may have pivotal roles in the progression of ESCC.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., & Liu, H. (2020). Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells. Oncology Letters, 20, 1163-1170. https://doi.org/10.3892/ol.2020.11654
MLA
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20.2 (2020): 1163-1170.
Chicago
Ren, Z., Geng, J., Xiong, C., Li, X., Li, Y., Li, J., Liu, H."Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells". Oncology Letters 20, no. 2 (2020): 1163-1170. https://doi.org/10.3892/ol.2020.11654