Open Access

IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer

  • Authors:
    • Samina Dongol
    • Qing Zhang
    • Chunping Qiu
    • Chenggong Sun
    • Zhiwei Zhang
    • Huan Wu
    • Beihua Kong
  • View Affiliations

  • Published online on: May 21, 2020     https://doi.org/10.3892/ol.2020.11664
  • Pages: 1179-1192
  • Copyright: © Dongol et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ovarian cancer is a type of gynecological cancer with the highest mortality rate worldwide. Due to a lack of effective screening methods, most cases are diagnosed at later stages where the survival rates are poor. Thus, it is termed a ‘silent killer’ and is the most lethal of all the malignancies in women. IQ motif containing GTPase Activating Protein 3 (IQGAP3) is a member of the Rho family of GTPases, and plays a crucial role in the development and progression of several types of cancer. The aim of the present study was to investigate the oncogenic functions and mechanisms of IQGAP3 on the proliferation and metastasis of high‑grade serous ovarian cancer (HGSOC). Therefore, the expression levels of IQGAP3 in HGSOC and normal tissue samples were compared, and IQGAP3 knockdown was performed to examine its functional role using various in vitro and in vivo experiments. It was demonstrated that the expression of IQGAP3 was upregulated in HGSOC tissues compared with the healthy tissues; this differential expression was also observed in the ovarian cancer cell lines. Functional experimental results suggested that IQGAP3 silencing significantly reduced proliferation, migration and invasion in ovarian cancer cell lines. Moreover, in vivo experimental findings validated the in vitro results, where the tumorigenic and metastatic capacities of IQGAP3‑silenced cells were significantly lower in the nude mice compared with the mice implanted with the control cells. Furthermore, knockdown of IQGAP3 resulted in increased apoptosis, and the effects of IQGAP3 expression on various epithelial‑mesenchymal transition markers were identified, suggesting a possible mechanism associated with the role of IQGAP3 in metastasis. The effect of IQGAP3 silencing on chemosensitivity towards olaparib was also assessed. Collectively, the present results indicated that IQGAP3 is a potential diagnostic and prognostic marker, and a putative therapeutic target of HGSOC.

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August-2020
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Copy and paste a formatted citation
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Spandidos Publications style
Dongol S, Zhang Q, Qiu C, Sun C, Zhang Z, Wu H and Kong B: IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer. Oncol Lett 20: 1179-1192, 2020
APA
Dongol, S., Zhang, Q., Qiu, C., Sun, C., Zhang, Z., Wu, H., & Kong, B. (2020). IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer. Oncology Letters, 20, 1179-1192. https://doi.org/10.3892/ol.2020.11664
MLA
Dongol, S., Zhang, Q., Qiu, C., Sun, C., Zhang, Z., Wu, H., Kong, B."IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer". Oncology Letters 20.2 (2020): 1179-1192.
Chicago
Dongol, S., Zhang, Q., Qiu, C., Sun, C., Zhang, Z., Wu, H., Kong, B."IQGAP3 promotes cancer proliferation and metastasis in high‑grade serous ovarian cancer". Oncology Letters 20, no. 2 (2020): 1179-1192. https://doi.org/10.3892/ol.2020.11664