Overexpression of miR‑518b in non‑small cell lung cancer serves as a biomarker and facilitates tumor cell proliferation, migration and invasion
- Xinfang Zhang
- Ying Hu
- Cuixue Gong
- Chunjie Zhang
Affiliations: Clinical Laboratory, Qilu Hospital Huantai Branch, Zibo, Shandong 256400, P.R. China, Department of Blood Transfusion, Qilu Hospital Huantai Branch, Zibo, Shandong 256400, P.R. China, Outpatient Department, Zibo Central Hospital, Zibo, Shandong 255000, P.R. China, Clinical Laboratory, Zibo Central Hospital, Zibo, Shandong 255000, P.R. China
- Published online on: May 22, 2020 https://doi.org/10.3892/ol.2020.11667
Copyright: © Zhang
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Several microRNAs (miRNA/miR) have been reported to serve critical roles in tumorigenesis. The present study aimed to investigate miR‑518b expression in non‑small cell lung cancer (NSCLC), and determine its clinical significance and biological function in this malignancy. Reverse transcription‑quantitative PCR was performed to assess miR‑518b expression in NSCLC. The diagnostic value of miR‑518b was determined via a receiver operating characteristic curve, while its prognostic value was assessed using the Kaplan‑Meier method. Gain‑ and loss‑of‑function experiments were performed to determine the functional role of miR‑518b in NSCLC progression. The results demonstrated that miR‑518b expression was upregulated in NSCLC serum, tissues and cell lines compared with the corresponding normal controls. Furthermore, high miR‑518b expression was significantly associated with larger tumor size, lymph node metastasis and advanced TNM stage, as well as poor overall survival in patients with NSCLC. Serum miR‑518b expression was identified as a candidate diagnostic biomarker for NSCLC, with sensitivity of 88.1% and specificity of 81.7%. Furthermore, the cell experiments indicated that NSCLC cell proliferation, migration and invasion were enhanced following overexpression of miR‑518b; however, these effects were reversed following miR‑518b knockdown. Taken together, the results of the present study suggest that elevated miR‑518b expression in NSCLC serves a potential oncogenic role by facilitating tumor cell proliferation, migration and invasion, and thus may serve as a candidate diagnostic and prognostic biomarker.