MicroRNA‑147 targets BDNF to inhibit cell proliferation, migration and invasion in non‑small cell lung cancer

Li,Fang; Wang,Xianfang; Yang,Lingling

doi:10.3892/ol.2020.11715

MicroRNA‑147 targets BDNF to inhibit cell proliferation, migration and invasion in non‑small cell lung cancer

Authors:
- Fang Li
- Xianfang Wang
- Lingling Yang
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Affiliations: Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276500, P.R. China
Published online on: June 9, 2020 https://doi.org/10.3892/ol.2020.11715
Pages: 1931-1937
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lung cancer is one of the most common cancers that threaten human life and health. Recently, microRNAs (miRNAs) have been shown to play a unique role in many malignancies. Although the dysregulation of miR‑147 has been detected in non‑small cell lung cancer (NSCLC), the biological function of miR‑147 is still unknown in NSCLC. The expression of miR‑147 was observed by real‑time quantitative polymerase chain reaction (RT‑qPCR). Methyl thiazolyl tetrazolium (MTT) and Transwell assays were used to investigate the function of miR‑147 in NSCLC. Target genes of miR‑147 were verified using dual luciferase reporter assay. Western blot analysis was used to explore the PI3K/AKT pathway. The expression of miR‑147 was decreased in NSCLC tissues, which was associated with poor prognosis in NSCLC patients. Furthermore, overexpression of miR‑147 inhibited the viability and metastasis of NSCLC cells. In addition, miR‑147 inhibited epithelial‑mesenchymal transition (EMT) and inactivated the PI3K/AKT pathway in NSCLC. Furthermore, miR‑147 directly targets brain‑derived neurotrophic factor (BDNF) and negatively regulates BDNF expression in NSCLC. Upregulation of BDNF attenuated the inhibitory effect of miR‑147 in NSCLC.In conclusion, miR‑147 inhibits cell proliferation, migration and invasion in NSCLC through suppressing BDNF expression.

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