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Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma

  • Authors:
    • Weirong Yao
    • Kaiyu Wang
    • Yu Jiang
    • Zhufeng Huang
    • Yiyun Huang
    • Huihui Yan
    • Suhong Huang
    • Min Chen
    • Jian Liao
  • View Affiliations / Copyright

    Affiliations: Institute for Laboratory Medicine, The First Hospital of Longhai, Zhangzhou, Fujian 363199, P.R. China, Institute for Laboratory Medicine, Fuzhou General Hospital of Nanjing Command (The 900th Hospital of Joint Logistic Support Force People's Liberation Army), Fuzhou, Fujian 350003, P.R. China, Clinical Laboratory, Fuzhou Second Hospital (Fuzhou Integrated Traditional Chinese and Modern Medicine Hospital of Fujian Province, Fuzhou Second Hospital Affiliated to Xiamen University), Fuzhou, Fujian 350007, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1597-1606
    |
    Published online on: June 11, 2020
       https://doi.org/10.3892/ol.2020.11727
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Abstract

Our previous study reported a method of using matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry to analyze the association between abnormal fucosylation of serum glycoproteins and the progression of hepatitis B virus (HBV)‑associated hepatocellular carcinoma (HCC). In the present study, the aforementioned method was improved by focusing on fucosylated glycoproteins <10 kD, classification models were established and blind tests were performed on an enlarged sample size (n=299). According to the present results, the classification models had a sensitivity and specificity of 74.31 and 76.32%, respectively, to identify HCC among all serum samples, 81.65 and 83.08%, respectively, to distinguish HCC from HBV‑associated cirrhosis and chronic hepatitis Band 88.99 and 84.62%, respectively, to distinguish HCC from HBV‑associated cirrhosis. When combined with α‑fetoprotein (AFP) measurements (AFP >20 ng/ml), the sensitivity and specificity of the models were significantly elevated to 80.73 and 87.37%, 87.16 and 90.00%, and 92.66 and 93.84%, respectively. In addition, the HBV‑HCC vs. HBV‑cirrhosis classification model was used to analyze serum samples collected from 9 patients with cirrhosis 1 year before they were diagnosed with HCC, and from 6 patients who had cirrhosis but developed no signs of HCC for the following 3 years. The model identified 7 patients (77.78%) with no significant clinical symptoms of HCC, and gave no false positive results, demonstrating that the classification models established in the present study may be useful for the early diagnosis of HCC. After isolation and purification, two proteins with differential expression were identified as isoform 1 of inter‑α‑trypsin inhibitor heavy chain 4 precursor, and thymosin β‑4‑like protein 3. These may be used as candidate markers for HCC diagnosis. Additionally, the present study indicates that defucosylation of serum glycoproteins may occur during the development and progression of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yao W, Wang K, Jiang Y, Huang Z, Huang Y, Yan H, Huang S, Chen M and Liao J: Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma. Oncol Lett 20: 1597-1606, 2020.
APA
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H. ... Liao, J. (2020). Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma. Oncology Letters, 20, 1597-1606. https://doi.org/10.3892/ol.2020.11727
MLA
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H., Huang, S., Chen, M., Liao, J."Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma". Oncology Letters 20.2 (2020): 1597-1606.
Chicago
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H., Huang, S., Chen, M., Liao, J."Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma". Oncology Letters 20, no. 2 (2020): 1597-1606. https://doi.org/10.3892/ol.2020.11727
Copy and paste a formatted citation
x
Spandidos Publications style
Yao W, Wang K, Jiang Y, Huang Z, Huang Y, Yan H, Huang S, Chen M and Liao J: Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma. Oncol Lett 20: 1597-1606, 2020.
APA
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H. ... Liao, J. (2020). Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma. Oncology Letters, 20, 1597-1606. https://doi.org/10.3892/ol.2020.11727
MLA
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H., Huang, S., Chen, M., Liao, J."Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma". Oncology Letters 20.2 (2020): 1597-1606.
Chicago
Yao, W., Wang, K., Jiang, Y., Huang, Z., Huang, Y., Yan, H., Huang, S., Chen, M., Liao, J."Serum profile of low molecular weight fucosylated glycoproteins for early diagnosis of hepatocellular carcinoma". Oncology Letters 20, no. 2 (2020): 1597-1606. https://doi.org/10.3892/ol.2020.11727
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