SDF‑1/CXCR4 induces cell invasion through CD147 in squamous cell carcinoma of the hypopharynx
- Satoshi Toyoma
- Shinsuke Suzuki
- Yohei Kawasaki
- Takechiyo Yamada
Affiliations: Department of Otorhinolaryngology and Head and Neck Surgery, Akita University Graduate School of Medicine, Akita 010‑8543, Japan
- Published online on: June 17, 2020 https://doi.org/10.3892/ol.2020.11744
Copyright: © Toyoma
et al. This is an open access article distributed under the
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Hypopharyngeal squamous cell carcinoma (SCC) has a poor prognosis due to local invasion and metastasis. The chemokine receptor CXC chemokine receptor type 4 (CXCR4) and its ligand, stromal cell‑derived factor 1 (SDF‑1), play roles in tumor progression through unclear mechanisms. For the present study, we used a hypopharyngeal SCC cell line, FaDu, expressing CXCR4. We found that SDF‑1 promotes migration and invasion of the FaDu cells. In addition, AMD3100, a specific antagonist of CXCR4, inhibited the binding of SDF‑1 to CXCR4, resulting in a significant decrease in the FaDu cell migration induced by SDF‑1. Stimulation of CXCR4 with SDF‑1 induced an increase in the expression of CD147, a cell membrane protein; and this CD147 upregulation was abrogated by AMD3100. CD147 function‑blocking antibodies also abolished the SDF‑1‑induced FaDu invasiveness. Our results suggested that SDF‑1/CXCR4 mediate hypopharyngeal SCC cell migration and that CD147 is involved in the SDF‑1/CXCR4‑related tumor progression.