Open Access

Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells

  • Authors:
    • Xiliang Zhang
    • Lian Duan
    • Yuxing Zhang
    • Huibin Zhao
    • Xiaodong Yang
    • Chaojun Zhang
  • View Affiliations

  • Published online on: June 17, 2020     https://doi.org/10.3892/ol.2020.11747
  • Pages: 1945-1951
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression level of Fibulin-2 gene in breast cancer cells was evaluated to explore the impact of Fibulin‑2 gene on the proliferation, migration and invasion of breast cancer cells. MDA‑MB‑231, BT483, MCF‑7 and SK‑BR‑3 breast cancer cells were cultured in vitro. Then, expression of Fibulin‑2 in cells was upregulated and downregulated using ribonucleic acid interference (RNAi) and lentiviral transfection techniques, respectively. Thereafter, expression levels of Fibulin‑2 messenger RNA (mRNA) and protein were measured via quantitative real‑time reverse transcription‑polymerase chain reaction and western blotting, respectively. Cell Counting Kit‑8 assay was applied to detect the proliferation ability, and wound healing assay was performed to determine the effect of transfection on the metastatic capacity of cells. The influence of transfection on the invasive ability of breast cancer cells was detected through Transwell chamber assay. MDA‑MB‑231 and MCF‑7 cells did not express Fibulin‑2, while BT483 and SK‑BR‑3 cells expressed Fibulin‑2. Expression of Fibulin‑2 mRNA and protein in SK‑BR‑3 Fibulin‑2 siRNA group was significantly lower than that in SK‑BR‑3 NC siRNA group 48 h after transfection (P<0.01), while the expression of Fibulin‑2 mRNA and protein in MDA‑MB‑231 Fibulin‑2 lentiviral transfection (LAP) group was significantly higher than that in MDA‑MB‑231 NC LAP group. Compared with the MDA‑MB‑231 NC LAP group, the cell proliferation, migration and invasion ability of MDA‑MB‑231 Fibulin‑2 LAP group were weakened. The tumor volume and weight of the MDA‑MB‑231 Fibulin‑2 LAP group were significantly lower than those of the MDA‑MB‑231 NC LAP group. Low expression of Fibulin‑2 is able to promote proliferation, migration and invasion of breast cancer cells, and can reduce the rate of tumor growth in nude mice. Therefore, Fibulin‑2 may be a potential therapeutic target and an indicator of prognosis for breast cancer.

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August-2020
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Copy and paste a formatted citation
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Spandidos Publications style
Zhang X, Duan L, Zhang Y, Zhao H, Yang X and Zhang C: Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncol Lett 20: 1945-1951, 2020
APA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., & Zhang, C. (2020). Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncology Letters, 20, 1945-1951. https://doi.org/10.3892/ol.2020.11747
MLA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20.2 (2020): 1945-1951.
Chicago
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20, no. 2 (2020): 1945-1951. https://doi.org/10.3892/ol.2020.11747