Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells

  • Authors:
    • Xiliang Zhang
    • Lian Duan
    • Yuxing Zhang
    • Huibin Zhao
    • Xiaodong Yang
    • Chaojun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Navy General Hospital, Beijing 100048, P.R. China, Department of Endocrinology, Peking Union Medical College Hospital, Beijing 100730, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1945-1951
    |
    Published online on: June 17, 2020
       https://doi.org/10.3892/ol.2020.11747
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Expression level of Fibulin-2 gene in breast cancer cells was evaluated to explore the impact of Fibulin‑2 gene on the proliferation, migration and invasion of breast cancer cells. MDA‑MB‑231, BT483, MCF‑7 and SK‑BR‑3 breast cancer cells were cultured in vitro. Then, expression of Fibulin‑2 in cells was upregulated and downregulated using ribonucleic acid interference (RNAi) and lentiviral transfection techniques, respectively. Thereafter, expression levels of Fibulin‑2 messenger RNA (mRNA) and protein were measured via quantitative real‑time reverse transcription‑polymerase chain reaction and western blotting, respectively. Cell Counting Kit‑8 assay was applied to detect the proliferation ability, and wound healing assay was performed to determine the effect of transfection on the metastatic capacity of cells. The influence of transfection on the invasive ability of breast cancer cells was detected through Transwell chamber assay. MDA‑MB‑231 and MCF‑7 cells did not express Fibulin‑2, while BT483 and SK‑BR‑3 cells expressed Fibulin‑2. Expression of Fibulin‑2 mRNA and protein in SK‑BR‑3 Fibulin‑2 siRNA group was significantly lower than that in SK‑BR‑3 NC siRNA group 48 h after transfection (P<0.01), while the expression of Fibulin‑2 mRNA and protein in MDA‑MB‑231 Fibulin‑2 lentiviral transfection (LAP) group was significantly higher than that in MDA‑MB‑231 NC LAP group. Compared with the MDA‑MB‑231 NC LAP group, the cell proliferation, migration and invasion ability of MDA‑MB‑231 Fibulin‑2 LAP group were weakened. The tumor volume and weight of the MDA‑MB‑231 Fibulin‑2 LAP group were significantly lower than those of the MDA‑MB‑231 NC LAP group. Low expression of Fibulin‑2 is able to promote proliferation, migration and invasion of breast cancer cells, and can reduce the rate of tumor growth in nude mice. Therefore, Fibulin‑2 may be a potential therapeutic target and an indicator of prognosis for breast cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Ducros E, Berthaut A, Mirshahi P, Lemarchand S, Soria J, Legeais JM and Mirshahi M: Expression of extracellular matrix proteins fibulin-1 and fibulin-2 by human corneal fibroblasts. Curr Eye Res. 32:481–490. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Wight TN: The extracellular matrix and atherosclerosis. Curr Opin Lipidol. 6:326–334. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Serra N, Rosales R, Masana L and Vallvé JC: Simvastatin increases fibulin-2 expression in human coronary artery smooth muscle cells via RhoA/Rho-kinase signaling pathway inhibition. PLoS One. 10:e01338752015. View Article : Google Scholar : PubMed/NCBI

4 

Aspberg A, Adam S, Kostka G, Timpl R and Heinegård D: Fibulin-1 is a ligand for the C-type lectin domains of aggrecan and versican. J Biol Chem. 274:20444–20449. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Olin AI, Mörgelin M, Sasaki T, Timpl R, Heinegård D and Aspberg A: The proteoglycans aggrecan and Versican form networks with fibulin-2 through their lectin domain binding. J Biol Chem. 276:1253–1261. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Kwan ML, Kushi LH, Weltzien E, Maring B, Kutner SE, Fulton RS, Lee MM, Ambrosone CB and Caan BJ: Epidemiology of breast cancer subtypes in two prospective cohort studies of breast cancer survivors. Breast Cancer Res. 11:R312009. View Article : Google Scholar : PubMed/NCBI

7 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Chen WQ, Zeng HM, Zheng RS, Zhang SW and He J: Cancer incidence and mortality in China, 2007. Chin J Cancer Res. 24:1–8. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Werb Z: ECM and cell surface proteolysis: Regulating cellular ecology. Cell. 91:439–442. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Wu TS and Hammond GL: Naturally occurring mutants inform SHBG structure and function. Mol Endocrinol. 28:1026–1038. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Shamay M, Barak O and Shaul Y: HBXAP, a novel PHD-finger protein, possesses transcription repression activity. Genomics. 79:523–529. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Whiteaker JR, Zhang H, Zhao L, Wang P, Kelly-Spratt KS, Ivey RG, Piening BD, Feng LC, Kasarda E, Gurley KE, et al: Integrated pipeline for mass spectrometry-based discovery and confirmation of biomarkers demonstrated in a mouse model of breast cancer. J Proteome Res. 6:3962–3975. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Hill VK, Hesson LB, Dansranjavin T, Dallol A, Bieche I, Vacher S, Tommasi S, Dobbins T, Gentle D, Euhus D, et al: Identification of 5 novel genes methylated in breast and other epithelial cancers. Mol Cancer. 9:512010. View Article : Google Scholar : PubMed/NCBI

14 

Fontanil T, Rúa S, Llamazares M, Moncada-Pazos A, Quirós PM, García-Suárez O, Vega JA, Sasaki T, Mohamedi Y, Esteban MM, et al: Interaction between the ADAMTS-12 metalloprotease and fibulin-2 induces tumor-suppressive effects in breast cancer cells. Oncotarget. 5:1253–1264. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Tian H, Liu J, Chen J, Gatza ML and Blobe GC: Fibulin-3 is a novel TGF-β pathway inhibitor in the breast cancer microenvironment. Oncogene. 34:5635–5647. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Chan SW, Lim CJ, Guo K, Ng CP, Lee I, Hunziker W, Zeng Q and Hong W: A role for TAZ in migration, invasion, and tumorigenesis of breast cancer cells. Cancer Res. 68:2592–2598. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Ibrahim AM, Sabet S, El-Ghor AA, Kamel N, Anis SE, Morris JS and Stein T: Fibulin-2 is required for basement membrane integrity of mammary epithelium. Sci Rep. 8:141392018. View Article : Google Scholar : PubMed/NCBI

18 

Tan H, Zhang J, Fu D and Zhu Y: Loss of fibulin-2 expression is involved in the inhibition of breast cancer invasion and forms a new barrier in addition to the basement membrane. Oncol Lett. 14:2663–2668. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Fontanil T, Álvarez-Teijeiro S, Villaronga MÁ, Mohamedi Y, Solares L, Moncada-Pazos A, Vega JA, García-Suárez O, Pérez-Basterrechea M, García-Pedrero JM, et al: Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells. Oncotarget. 8:13716–13729. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Dunwell TL, Hesson LB, Pavlova T, Zabarovska V, Kashuba V, Catchpoole D, Chiaramonte R, Brini AT, Griffiths M, Maher ER, et al: Epigenetic analysis of childhood acute lymphoblastic leukemia. Epigenetics. 4:185–193. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Mesri EA, Cesarman E and Boshoff C: Kaposi's sarcoma and its associated herpesvirus. Nat Rev Cancer. 10:707–719. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Alcendor DJ, Knobel S, Desai P, Zhu WQ and Hayward GS: KSHV regulation of fibulin-2 in Kaposi's sarcoma: Implications for tumorigenesis. Am J Pathol. 179:1443–1454. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Senapati S, Gnanapragassam VS, Moniaux N, Momi N and Batra SK: Role of MUC4-NIDO domain in the MUC4-mediated metastasis of pancreatic cancer cells. Oncogene. 31:3346–3356. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Duan L, Zhang Y, Zhao H, Yang X and Zhang C: Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncol Lett 20: 1945-1951, 2020.
APA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., & Zhang, C. (2020). Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncology Letters, 20, 1945-1951. https://doi.org/10.3892/ol.2020.11747
MLA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20.2 (2020): 1945-1951.
Chicago
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20, no. 2 (2020): 1945-1951. https://doi.org/10.3892/ol.2020.11747
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Duan L, Zhang Y, Zhao H, Yang X and Zhang C: Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncol Lett 20: 1945-1951, 2020.
APA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., & Zhang, C. (2020). Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells. Oncology Letters, 20, 1945-1951. https://doi.org/10.3892/ol.2020.11747
MLA
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20.2 (2020): 1945-1951.
Chicago
Zhang, X., Duan, L., Zhang, Y., Zhao, H., Yang, X., Zhang, C."Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells". Oncology Letters 20, no. 2 (2020): 1945-1951. https://doi.org/10.3892/ol.2020.11747
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team