Open Access

Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer

  • Authors:
    • Yushi Imai
    • Tetsuhiro Chiba
    • Takayuki Kondo
    • Hiroaki Kanzaki
    • Kengo  Kanayama
    • Junjie  Ao
    • Ryuta Kojima
    • Yuko Kusakabe
    • Masato  Nakamura
    • Tomoko  Saito
    • Ryo Nakagawa
    • Eiichiro Suzuki
    • Shingo Nakamoto
    • Ryosuke  Muroyama
    • Akinobu Tawada
    • Tomoaki Matsumura
    • Tomoo  Nakagawa
    • Jun Kato
    • Ai Kotani
    • Hisahiro Matsubara
    • Naoya Kato
  • View Affiliations

  • Published online on: June 19, 2020     https://doi.org/10.3892/ol.2020.11757
  • Pages: 2161-2168
  • Copyright: © Imai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Programmed death‑ligand 1 (PD‑L1) plays an essential role in tumor cell escape from anti‑tumor immunity in various types of cancer, including gastric cancer (GC). The present study investigated the intracellular and membrane‑bound expression of PD‑L1 in the GC cell lines MKN1, MKN74, KATO III and OCUM‑1. Furthermore, soluble PD‑L1 (sPD‑L1) level in the supernatant of GC cells and the serum of patients with GC and healthy controls was determined by ELISA. Interferon (IFN)‑γ treatment of cells resulted in increased cytoplasmic expression of PD‑L1 in GC cells in a dose‑dependent manner, except for MKN74 cells; however, there was no association between tumor necrosis factor‑α treatment and enhanced PD‑L1 expression. Concordant with these findings, results from flow cytometry analysis demonstrated that membrane‑bound PD‑L1 expression was also increased following GC cell treatment with IFN‑γ in a dose‑dependent manner. In addition, significant sPD‑L1 overproduction was observed only in the culture supernatant of OCUM‑1 cells. Serum level of sPD‑L1 was significantly increased in patients with GC, in particular in stage IV patients, compared with healthy controls. In conclusion, the present study demonstrated that IFN‑γ treatment increased the intracellular and membrane‑bound PD‑L1 expression in GC cells. In addition, sPD‑L1 was detected not only in the supernatant of GC cells but also in the serum of patients with GC. Further investigation on the underlying mechanism of regulation of PD‑L1 expression and sPD‑L1 production is required.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Imai Y, Chiba T, Kondo T, Kanzaki H, Kanayama K, Ao J, Kojima R, Kusakabe Y, Nakamura M, Saito T, Saito T, et al: Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer. Oncol Lett 20: 2161-2168, 2020
APA
Imai, Y., Chiba, T., Kondo, T., Kanzaki, H., Kanayama, K., Ao, J. ... Kato, N. (2020). Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer. Oncology Letters, 20, 2161-2168. https://doi.org/10.3892/ol.2020.11757
MLA
Imai, Y., Chiba, T., Kondo, T., Kanzaki, H., Kanayama, K., Ao, J., Kojima, R., Kusakabe, Y., Nakamura, M., Saito, T., Nakagawa, R., Suzuki, E., Nakamoto, S., Muroyama, R., Tawada, A., Matsumura, T., Nakagawa, T., Kato, J., Kotani, A., Matsubara, H., Kato, N."Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer". Oncology Letters 20.3 (2020): 2161-2168.
Chicago
Imai, Y., Chiba, T., Kondo, T., Kanzaki, H., Kanayama, K., Ao, J., Kojima, R., Kusakabe, Y., Nakamura, M., Saito, T., Nakagawa, R., Suzuki, E., Nakamoto, S., Muroyama, R., Tawada, A., Matsumura, T., Nakagawa, T., Kato, J., Kotani, A., Matsubara, H., Kato, N."Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer". Oncology Letters 20, no. 3 (2020): 2161-2168. https://doi.org/10.3892/ol.2020.11757