Open Access

Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells

  • Authors:
    • Jiajia Jin
    • Zixiao Huang
    • Xiaoyan Lu
    • Shengrong Wu
    • Mei'e Jia
    • Xin Li
    • Zhiyong Li
    • Xiangyi He
  • View Affiliations

  • Published online on: June 24, 2020     https://doi.org/10.3892/ol.2020.11764
  • Pages: 2378-2386
  • Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral squamous cell carcinoma (OSCC) is the most prevalent form of malignant tumour in the oral cavity and its early detection is critical for improving the prognosis of affected patients. The present study aimed to isolate and confirm exosomes derived from the supernatant of the OSCC cell line CAL‑27 and human oral epithelial cells (HOECs), analyze long non‑coding RNA (lncRNA) expression profiles and determine the diagnostic value based on bioinformatics analyses. The results indicated that the particles isolated from the supernatant of CAL‑27 and HOECs were either round or oval, had a size range of 30‑150 nm and were enriched with ALG‑2 interacting protein X (ALIX) and tumour susceptibility 101 proteins (TSG101). These characteristics confirmed that these particles were exosomes. Three lncRNAs (NR‑026892.1, NR‑126435.1 and NR‑036586.1) were selected as potential diagnostic biomarkers for OSCC. The expression levels of the selected lncRNAs were significantly different in CAL‑27‑exo vs. HOEC‑exo, as well as in whole cells (CAL‑27 vs. HOECs) (P<0.001). The expression levels of the three lncRNAs confirmed by quantitative PCR were consistent with the sequencing data. In conclusion, various lncRNAs were aberrantly expressed between cancerous and non‑cancerous exosomes, suggesting that they may serve as biomarkers for cancer

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jin J, Huang Z, Lu X, Wu S, Jia M, Li X, Li Z and He X: Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells. Oncol Lett 20: 2378-2386, 2020
APA
Jin, J., Huang, Z., Lu, X., Wu, S., Jia, M., Li, X. ... He, X. (2020). Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells. Oncology Letters, 20, 2378-2386. https://doi.org/10.3892/ol.2020.11764
MLA
Jin, J., Huang, Z., Lu, X., Wu, S., Jia, M., Li, X., Li, Z., He, X."Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells". Oncology Letters 20.3 (2020): 2378-2386.
Chicago
Jin, J., Huang, Z., Lu, X., Wu, S., Jia, M., Li, X., Li, Z., He, X."Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells". Oncology Letters 20, no. 3 (2020): 2378-2386. https://doi.org/10.3892/ol.2020.11764