Open Access

Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation

  • Authors:
    • Li Jiang
    • Ting Chen
    • Li Xiong
    • Ji‑Hao Xu
    • Ai‑Yu Gong
    • Bin Dai
    • Ganlin Wu
    • Kenny Zhu
    • Eugene Lu
    • Nicholas William Mathy
    • Xian‑Ming Chen
  • View Affiliations

  • Published online on: July 1, 2020     https://doi.org/10.3892/ol.2020.11794
  • Pages: 2191-2198
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

N6‑methyladenosine (m6A) RNA modification regulates multiple biological functions. Methyltransferase like 3 (METTL3), one of the major N6‑methyltransferases, is highly expressed in gastric cancer, but its potential role in disease is unclear. The current study knocked out METTL3 (METTL3‑KO) in human gastric cancer AGS cells using CRISPR/Cas9. METTL3‑KO AGS cells exhibited decreased m6A methylation levels. A significant inhibition of cell proliferation was observed in METTL3‑KO AGS cells. Silencing METTL3 in AGS cells altered the expression profile of many effector molecules that were previously demonstrated to serve key roles in AGS cell proliferation, including the suppressor of cytokine signaling (SOCS) family of proteins. The results further demonstrated that SOCS2 upregulation in METTL3‑KO AGS cells was associated with a decreased RNA decay rate. Furthermore, SOCS2 KO or SOCS2 overexpression caused a significant increase and decrease in AGS cell proliferation, respectively. The current data suggested that METTL3‑KO in gastric cancer cells resulted in the suppression of cell proliferation by inducing SOCS2, suggesting a potential role of elevated METTL3 expression in gastric cancer progression.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jiang L, Chen T, Xiong L, Xu JH, Gong AY, Dai B, Wu G, Zhu K, Lu E, Mathy NW, Mathy NW, et al: Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation. Oncol Lett 20: 2191-2198, 2020
APA
Jiang, L., Chen, T., Xiong, L., Xu, J., Gong, A., Dai, B. ... Chen, X. (2020). Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation. Oncology Letters, 20, 2191-2198. https://doi.org/10.3892/ol.2020.11794
MLA
Jiang, L., Chen, T., Xiong, L., Xu, J., Gong, A., Dai, B., Wu, G., Zhu, K., Lu, E., Mathy, N. W., Chen, X."Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation". Oncology Letters 20.3 (2020): 2191-2198.
Chicago
Jiang, L., Chen, T., Xiong, L., Xu, J., Gong, A., Dai, B., Wu, G., Zhu, K., Lu, E., Mathy, N. W., Chen, X."Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation". Oncology Letters 20, no. 3 (2020): 2191-2198. https://doi.org/10.3892/ol.2020.11794