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Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases

  • Authors:
    • Shih‑Chang Chuang
    • Ching‑Wen Huang
    • Yi‑Ting Chen
    • Cheng‑Jen Ma
    • Hsiang‑Lin Tsai
    • Tsung‑Kun Chang
    • Wei‑Chih Su
    • Wen‑Hung Hsu
    • Chao‑Hung Kuo
    • Jaw‑Yuan Wang
  • View Affiliations / Copyright

    Affiliations: Division of General and Digestive Surgery, Department of Surgery; Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan, R.O.C., Department of Surgery, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan, R.O.C., Department of Pathology; Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan, R.O.C., Division of Colorectal Surgery, Department of Surgery, Kaohsiung Medical University, Kaohsiung 807, Taiwan, R.O.C., Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital and Kaohsiung Medical University, Kaohsiung 807, Taiwan, R.O.C.
    Copyright: © Chuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2119-2130
    |
    Published online on: July 1, 2020
       https://doi.org/10.3892/ol.2020.11795
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Abstract

It has been reported that 20‑25% of patients with colorectal cancer (CRC) have metastases at the time of diagnosis. Liver and lung are the most common metastatic sites. The aim of the present study was to investigate the association of KRAS and NRAS mutations with clinicopathological features and prognosis of patients with initial liver‑metastasis only (LiM‑only) or lung‑metastasis only (LuM‑only) metastatic CRC (mCRC). Overall, 166 patients with CRC with initial LiM‑only (n=124) and LuM‑only (n=42) were retrospectively analyzed from January 2014 to December 2017. The median follow‑up time was 19.2 months (1.0‑57.1 months). Patient characteristics at diagnosis were collected. Genomic DNA was isolated from frozen primary CRC tissues for targeting KRAS and NRAS. Patients with LuM‑only were significantly older compared with those with LiM‑only (65.5 vs. 61.5 years; P=0.05). There was no significant differences between the LiM‑only and LuM‑only groups in terms of sex, location of the primary tumor, serum carcinoembryonic antigen level, histological grade and RAS mutation status. KRAS mutations were detected in 43 (41.0%) patients with LiM‑only and 13 (35.1%) patients with LuM‑only. The overall survival time (OS) of LuM‑only was more favorable compared with that of patients with LiM‑only (44.5 vs. 24.7 months); however, there was no significant difference (P=0.095). The progression‑free survival (PFS) and OS in the RAS wild‑type group were significantly improved compared with the RAS mutant cohorts (P=0.004 and P=0.031, respectively) in the LiM‑only group. In patients with stage IV CRC, those with synchronous LiM‑only mCRC had a higher incidence of metastasis but a less favorable PFS and OS compared with patients with LuM‑only. RAS mutation status exhibited a significant association with the survival outcome in patients with LiM‑only mCRC.
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View References

1 

Bray F, Ferly J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Millikan KW, Staren ED and Doolas A: Invasive therapy of metastatic colorectal cancer to the liver. Surg Clin North Am. 77:27–48. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Nitzkorski JR, Farma JM, Watson JC, Siripurapu V, Zhu F, Matteotti RS and Sigurdson ER: Outcome and natural history of patients with stage IV colorectal cancer receiving chemotherapy without primary tumor resection. Ann Surg Oncol. 19:379–383. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Prasanna T, Karapetis CS, Roder D, Tie J, Padbury R, Price T, Wong R, Shapiro J, Nott L, Lee M, et al: The survival outcome of patients with metastatic colorectal cancer based on the site of metastases and the impact of molecular markers and site of primary cancer on metastatic pattern. Acta Oncol. 57:1438–1444. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Qiu M, Hu J, Yang D, Cosgrove DP and Xu R: Pattern of distant metastases in colorectal cancer: A SEER based study. Oncotarget. 6:38658–38666. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Zizzo M, Galeone C, Braglia L, Ugoletti L, Siciliani A, Nachira D, Margaritora S, Pedrazzoli C, Paci M, Lococo F, et al: Long-Term outcomes after surgical resection for synchronous or metachronous hepatic and pulmonary colorectal cancer metastases. Digestion. 101:144–155. 2020. View Article : Google Scholar : PubMed/NCBI

7 

Augestad KM, Merok MA and Ignatovic D: Tailored treatment of colorectal cancer: Surgical, molecular, and genetic considerations. Clin Med Insights Oncol. 11:11795549176907662017. View Article : Google Scholar : PubMed/NCBI

8 

Raskov H, Pommergaard HC, Burcharth J and Rosenberg J: Colorectal carcinogenesis-update and perspectives. World J Gastroenterol. 20:18151–18164. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Amado RG, Wolf M, Peeters M, Van Cutsem E, Siena S, Freeman DJ, Juan T, Sikorski R, Suggs S, Radinsky R, et al: Wild-type KRAS is required for panitumumab efficacy in patients with metastatic colorectal cancer. J Clin Oncol. 26:1626–1634. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Schirripa M, Cremolini C, Loupakis F, Morvillo M, Bergamo F, Zoratto F, Salvatore L, Antoniotti C, Marmorino F, Sensi E, et al: Role of NRAS mutations as prognostic and predictive markers in metastatic colorectal cancer. Int J Cancer. 136:83–90. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Roth AD, Tejpar S, Delorenzi M, Yan P, Fiocca R, Klingbiel D, Dietrich D, Biesmans B, Bodoky G, Barone C, et al: Prognostic role of KRAS and BRAF in stage II and III resected colon cancer: Results of the translational study on the PETACC-3, EORTC 40993, SAKK 60-00 trial. J Clin Oncol. 28:466–474. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Hutchins G, Southward K, Handley K, Magill L, Beaumont C, Stahlschmidt J, Richman S, Chambers P, Seymour M, Kerr D, et al: Value of mismatch repair, KRAS, and BRAF mutations in predicting recurrence and benefits from chemotherapy in colorectal cancer. J Clin Oncol. 29:1261–1270. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Zhang Y, Ma J, Zhang S, Deng G, Wu X, He J, Pei H, Shen H and Zeng S: A prognostic analysis of 895 cases of stage III colon cancer in different colon subsites. Int J Colorectal Dis. 30:1173–1183. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Fakih M and Wong R: Efficacy of the monoclonal antibody EGFR inhibitors for the treatment of metastatic colorectal cancer. Curr Oncol. 17 (Suppl 1):S3–S17. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Venook AP: Epidermal growth factor receptor-targeted treatment for advanced colorectal carcinoma. Cancer. 103:2435–2446. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Bibeau F, Boissiere-Michot F, Sabourin JC, Gourgou-Bourgade S, Radal M, Penault-Llorca F, Rochaix P, Arnould L, Bralet MP, Azria D and Ychou M: Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray. Virchows Arch. 449:281–287. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Liang Z, Zhang J, Zeng X, Gao J, Wu S and Liu T: Relationship between EGFR expression, copy number and mutation in lung adenocarcinomas. BMC Cancer. 10:3762010. View Article : Google Scholar : PubMed/NCBI

18 

Edge SB and Compton CC: The American Joint Committee on Cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Sambrook J and Russell DW: The condensed protocols from molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor. (NY). p. v. 8002006.

20 

Yen LC, Uen YH, Wu DC, Lu CY, Yu FJ, Wu IC, Lin SR and Wang JY: Activating KRAS mutations and overexpression of epidermal growth factor receptor as independent predictors in metastatic colorectal cancer patients treated with cetuximab. Ann Surg. 251:254–260. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Wang JY, Hsieh JS, Chen FM, Yeh CS, Alexandersen K, Huang TJ, Chen DC and Lin SR: High frequency of activated K-ras codon 15 mutant in colorectal carcinomas from Taiwanese patients. Int J Cancer. 107:387–393. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Huang CW, Chen YT, Tsai HL, Yeh YS, Su WC, Ma CJ, Tsai TN and Wang JY: EGFR expression in patients with stage III colorectal cancer after adjuvant chemotherapy and on cancer cell function. Oncotarget. 8:114663–114676. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Huang CW, Tsai HL, Chen YT, Huang CM, Ma CJ, Lu CY, Kuo CH, Wu DC, Chai CY and Wang JY: The prognostic values of EGFR expression and KRAS mutation in patients with synchronous or metachronous metastatic colorectal cancer. BMC Cancer. 13:5992013. View Article : Google Scholar : PubMed/NCBI

24 

Scartozzi M, Bearzi I, Berardi R, Mandolesi A, Fabris G and Cascinu S: Epidermal growth factor receptor (EGFR) status in primary colorectal tumors does not correlate with EGFR expression in related metastatic sites: Implications for treatment with EGFR-targeted monoclonal antibodies. J Clin Oncol. 1:4772–4778. 2004. View Article : Google Scholar

25 

Cancer Genome Atlas Network, . Comprehensive molecular characterization of human colon and rectal cancer. Nature. 487:330–337. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Liu Y, Sethi NS, Hinoue T, Schneider BG, Cherniack AD, Sanchez-Vega F, Seoane JA, Farshidfar F, Bowlby R, Islam M, et al: Comparative molecular analysis of gastrointestinal adenocarcinomas. Cancer Cell. 33:721–735.e8. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Wee P and Wang Z: Epidermal growth factor receptor cell proliferation signaling pathways. Cancers (Basel). 9:522017. View Article : Google Scholar

28 

Tan C and Du X: KRAS mutation testing in metastatic colorectal cancer. World J Gastroenterol. 18:5171–5180. 2012.PubMed/NCBI

29 

Lo Nigro C, Ricci V, Vivenza D, Granetto C, Fabozzi T, Miraglio E and Merlano MC: Prognostic and predictive biomarkers in metastatic colorectal cancer anti-EGFR therapy. World J Gastroenterol. 22:6944–6954. 2016. View Article : Google Scholar : PubMed/NCBI

30 

De Stefano A and Carlomagno C: Beyond KRAS: Predictive factors of the efficacy of anti-EGFR monoclonal antibodies in the treatment of metastatic colorectal cancer. World J Gastroenterol. 20:9732–9743. 2014. View Article : Google Scholar : PubMed/NCBI

31 

He XK, Wu W, Ding YE, Li Y, Sun LM and Si J: Different anatomical subsites of colon cancer and mortality: A population-based study. Gastroenterol Res Pract. 2018:71536852018. View Article : Google Scholar : PubMed/NCBI

32 

Engstrand J, Nilsson H, Stromberg C, Jonas E and Freedman J: Colorectal cancer liver metastases-a population-based study on incidence, management and survival. BMC Cancer. 18:782018. View Article : Google Scholar : PubMed/NCBI

33 

Kim HS, Heo JS, Lee J, Lee JY, Lee MY, Lim SH, Lee WY, Kim SH, Park YA, Cho YB, et al: The impact of KRAS mutations on prognosis in surgically resected colorectal cancer patients with liver and lung metastases: A retrospective analysis. BMC Cancer. 16:1202016. View Article : Google Scholar : PubMed/NCBI

34 

Yong ZZ, Ching GTH and Ching MTC: Metastatic profile of colorectal cancer: Interplay between primary tumor location and KRAS Status. J Surg Res. 246:325–334. 2020. View Article : Google Scholar : PubMed/NCBI

35 

Shankaran V, Obel J and Benson AB III: Predicting response to EGFR inhibitors in metastatic colorectal cancer: Current practice and future directions. Oncologist. 15:157–167. 2010. View Article : Google Scholar : PubMed/NCBI

36 

van Helden EJ, Menke-van der Houven van Oordt CW, Heymans MW, Ket JCF, van den Oord R and Verheul HMW: Optimal use of anti-EGFR monoclonal antibodies for patients with advanced colorectal cancer: A meta-analysis. Cancer Metastasis Rev. 36:395–406. 2017. View Article : Google Scholar : PubMed/NCBI

37 

Kato S, Okamura R, Mareboina M, Lee S, Goodman A, Patel SP, Fanta PT, Schwab RB, Vu P, Raymond VM, et al: Revisiting Epidermal Growth Factor Receptor (EGFR) Amplification as a Target for Anti-EGFR therapy: Analysis of cell-free circulating tumor DNA in patients with advanced malignancies. JCO Precis Oncol. 3:102019.

38 

Yang ZY, Shen WX, Hu XF, Zheng DY, Wu XY, Huang YF, Chen JZ, Mao C and Tang JL: EGFR gene copy number as a predictive biomarker for the treatment of metastatic colorectal cancer with anti-EGFR monoclonal antibodies: A meta-analysis. J Hematol Oncol. 5:522012. View Article : Google Scholar : PubMed/NCBI

39 

Brudvik KW, Kopetz SE, Li L, Conrad C, Aloia TA and Vauthey JN: Meta-analysis of KRAS mutations and survival after resection of colorectal liver metastases. Br J Surg. 102:1175–1183. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Shindoh J, Nishioka Y, Yoshioka R, Sugawara T, Sakamoto Y, Hasegawa K, Hashimoto M and Kokudo N: KRAS mutation status predicts Site-Specific recurrence and survival after resection of colorectal liver metastases irrespective of location of the primary lesion. Ann Surg Oncol. 23:1890–1896. 2016. View Article : Google Scholar : PubMed/NCBI

41 

Yaeger R, Cowell E, Chou JF, Gewirtz AN, Borsu L, Vakiani E, Solit DB, Rosen N, Capanu M, Ladanyi M and Kemeny N: RAS mutations affect pattern of metastatic spread and increase propensity for brain metastasis in colorectal cancer. Cancer. 121:1195–1203. 2015. View Article : Google Scholar : PubMed/NCBI

42 

Nagakubo Y, Hirotsu Y, Amemiya K, Oyama T, Mochizuki H and Omata M: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR. BMC Med Genomics. 12:1622019. View Article : Google Scholar : PubMed/NCBI

43 

Gilson P, Franczak C, Dubouis L, Husson M, Rouyer M, Demange J, Perceau M, Leroux A, Merlin JL, Harlé A, et al: Evaluation of KRAS, NRAS and BRAF hotspot mutations detection for patients with metastatic colorectal cancer using direct DNA pipetting in a fully-automated platform and next-generation sequencing for laboratory workflow optimisation. PLoS One. 14:e02192042019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chuang SC, Huang CW, Chen YT, Ma CJ, Tsai HL, Chang TK, Su WC, Hsu WH, Kuo CH, Wang JY, Wang JY, et al: Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases. Oncol Lett 20: 2119-2130, 2020.
APA
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T. ... Wang, J. (2020). Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases. Oncology Letters, 20, 2119-2130. https://doi.org/10.3892/ol.2020.11795
MLA
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T., Su, W., Hsu, W., Kuo, C., Wang, J."Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases". Oncology Letters 20.3 (2020): 2119-2130.
Chicago
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T., Su, W., Hsu, W., Kuo, C., Wang, J."Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases". Oncology Letters 20, no. 3 (2020): 2119-2130. https://doi.org/10.3892/ol.2020.11795
Copy and paste a formatted citation
x
Spandidos Publications style
Chuang SC, Huang CW, Chen YT, Ma CJ, Tsai HL, Chang TK, Su WC, Hsu WH, Kuo CH, Wang JY, Wang JY, et al: Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases. Oncol Lett 20: 2119-2130, 2020.
APA
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T. ... Wang, J. (2020). Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases. Oncology Letters, 20, 2119-2130. https://doi.org/10.3892/ol.2020.11795
MLA
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T., Su, W., Hsu, W., Kuo, C., Wang, J."Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases". Oncology Letters 20.3 (2020): 2119-2130.
Chicago
Chuang, S., Huang, C., Chen, Y., Ma, C., Tsai, H., Chang, T., Su, W., Hsu, W., Kuo, C., Wang, J."Effect of KRAS and NRAS mutations on the prognosis of patients with synchronous metastatic colorectal cancer presenting with liver‑only and lung‑only metastases". Oncology Letters 20, no. 3 (2020): 2119-2130. https://doi.org/10.3892/ol.2020.11795
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