Open Access

Adenovirus‑mediated overexpression of interleukin‑21 regulates the development of oral squamous cell carcinoma in vitro

  • Authors:
    • Hao Liu
    • Peng Liu
    • Di Sun
    • Dayuan Xing
    • Xiaoping Wang
    • Jinge Yang
    • Shengzhi Wang
  • View Affiliations

  • Published online on: July 7, 2020     https://doi.org/10.3892/ol.2020.11822
  • Pages: 3006-3014
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interleukin‑21 (IL‑21) is an important cytokine that is currently being investigated for its potential use in tumor immunotherapy in the future. In tumor cells, IL‑21 stimulates the immune response by increasing the cytotoxic activity of natural killer cells, B cells and CD8+ T cells, which in turn induces the apoptosis of tumor cells. The therapeutic effects of IL‑21 have been investigated in several types of disease and numerous clinical trials are in progress. The aim of the present study was to determine the role of IL‑21 in oral squamous cell carcinoma (OSCC) in vitro. IL‑21 expression was detected in OSCC tissues via RT‑qPCR, western blotting and immunohistochemistry analyses. The results demonstrated that IL‑21 protein expression decreased in OSCC tissues. IL‑21 was overexpressed using adenovirus in CAL‑27 cells. The Cell Counting Kit‑8 assay demonstrated that overexpression of IL‑21 inhibited cell proliferation. Furthermore, overexpression of IL‑21 inhibited cell migration, detected by the wound healing assay, and promoted cell apoptosis, detected by TUNEL staining and flow cytometry analysis. The results demonstrated that overexpression of IL‑21 inhibited activation of the JNK signaling pathway. In conclusion, the findings of the present study suggest that IL‑21 may function as a potent antitumor agent in OSCC.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Liu H, Liu P, Sun D, Xing D, Wang X, Yang J and Wang S: Adenovirus‑mediated overexpression of interleukin‑21 regulates the development of oral squamous cell carcinoma in vitro. Oncol Lett 20: 3006-3014, 2020
APA
Liu, H., Liu, P., Sun, D., Xing, D., Wang, X., Yang, J., & Wang, S. (2020). Adenovirus‑mediated overexpression of interleukin‑21 regulates the development of oral squamous cell carcinoma in vitro. Oncology Letters, 20, 3006-3014. https://doi.org/10.3892/ol.2020.11822
MLA
Liu, H., Liu, P., Sun, D., Xing, D., Wang, X., Yang, J., Wang, S."Adenovirus‑mediated overexpression of interleukin‑21 regulates the development of oral squamous cell carcinoma in vitro". Oncology Letters 20.3 (2020): 3006-3014.
Chicago
Liu, H., Liu, P., Sun, D., Xing, D., Wang, X., Yang, J., Wang, S."Adenovirus‑mediated overexpression of interleukin‑21 regulates the development of oral squamous cell carcinoma in vitro". Oncology Letters 20, no. 3 (2020): 3006-3014. https://doi.org/10.3892/ol.2020.11822