Open Access

Selection of a malignant subpopulation from a colorectal cancer cell line

  • Authors:
    • Pei‑Lun Lai
    • Ting‑Chun Chen
    • Chun‑Yen Feng
    • Hsuan Lin
    • Chi‑Hou Ng
    • Yun Chen
    • Michael Hsiao
    • Jean Lu
    • Hsiao‑Chun Huang
  • View Affiliations

  • Published online on: July 8, 2020     https://doi.org/10.3892/ol.2020.11829
  • Pages: 2937-2945
  • Copyright: © Lai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Colorectal cancer (CRC) is a leading cause of cancer‑associated mortality worldwide; therefore, there is an emerging need for novel experimental models that allow for the identification and validation of biomarkers for CRC‑specific progression. In the present study, a repeated sphere‑forming assay was used as a strategy to select a malignant subpopulation from a CRC cell line, namely HCT116. The assay was validated by confirming that canonical stemness markers were upregulated in the sphere state at every generation of the selection assay. The resulting subpopulation, after eight rounds of selection, exhibited increased sphere‑forming capacity in vitro and increased tumorigenicity in vivo. Furthermore, dipeptidase 1 (DPEP1) was identified as the major differentially expressed gene in the selected clone, and its depletion suppressed the elevated sphere‑forming capacity in vitro and tumorigenicity in vivo. Overall, the present study established an experimental strategy to isolate a malignant subpopulation from a CRC cell line. Additionally, results from the present model revealed that DPEP1 may serve as a promising prognostic biomarker for CRC.

Related Articles

Journal Cover

September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lai PL, Chen TC, Feng CY, Lin H, Ng CH, Chen Y, Hsiao M, Lu J and Huang HC: Selection of a malignant subpopulation from a colorectal cancer cell line. Oncol Lett 20: 2937-2945, 2020
APA
Lai, P., Chen, T., Feng, C., Lin, H., Ng, C., Chen, Y. ... Huang, H. (2020). Selection of a malignant subpopulation from a colorectal cancer cell line. Oncology Letters, 20, 2937-2945. https://doi.org/10.3892/ol.2020.11829
MLA
Lai, P., Chen, T., Feng, C., Lin, H., Ng, C., Chen, Y., Hsiao, M., Lu, J., Huang, H."Selection of a malignant subpopulation from a colorectal cancer cell line". Oncology Letters 20.3 (2020): 2937-2945.
Chicago
Lai, P., Chen, T., Feng, C., Lin, H., Ng, C., Chen, Y., Hsiao, M., Lu, J., Huang, H."Selection of a malignant subpopulation from a colorectal cancer cell line". Oncology Letters 20, no. 3 (2020): 2937-2945. https://doi.org/10.3892/ol.2020.11829