Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2020 Volume 20 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2020 Volume 20 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer

  • Authors:
    • Honggen Liu
    • Peiying Yang
    • Xiaojiang Li
    • Yingjie Jia
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 35
    |
    Published online on: July 22, 2020
       https://doi.org/10.3892/ol.2020.11898
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

There are few studies on the role of ring finger protein (RNF)180 in non‑small cell lung cancer (NSCLC). The present study investigated the expression of RNF180 in NSCLC and its associations with the clinical factors and prognosis of NSCLC. The mRNA and protein expression levels of RNF180 were detected via reverse transcription‑quantitative PCR and western blotting. Methylation‑specific PCR (MSP) analysis was utilized to detect the methylation of RNF180. RNF180 expression levels were analyzed via immunohistochemistry. The protein and mRNA expression levels of RNF180 were lower in NSCLC cell lines compared with in the non‑tumor cell line. Immunohistochemistry revealed that 64 patients that were negative for RNF180, while MSP detection analysis demonstrated that 60 patients exhibited RNF180 promoter methylation. The methylation status of RNF180 was significantly associated with RNF180 expression level. Among all factors evaluated, logistic regression analysis indicated that only T stage was significantly associated with RNF180 expression. Cox multivariate analysis demonstrated that RNF180 expression was an independent predictor of overall survival in patients with NSCLC. Methylation in the promoter of RNF180 was shown to reduce its expression levels. In summary, low RNF180 expression levels were associated with poor biological behaviors, thus RNF180 expression level may be used as a clinical marker to predict the prognosis of patients with NSCLC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Miranda-Filho A, Piñeros M and Bray F: The descriptive epidemiology of lung cancer and tobacco control: A global overview 2018. Salud Publica Mex. 61:219–229. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Li J, Zhu H, Sun L, Xu W and Wang X: Prognostic value of site-specific metastases in lung cancer: A population based study. J Cancer. 10:3079–3086. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Zhao W, Yu H, Han Z, Gao N, Xue J and Wang Y: Clinical significance of joint detection of serum CEA, SCCA, and bFGF in the diagnosis of lung cancer. Int J Clin Exp Pathol. 8:9506–9511. 2015.PubMed/NCBI

4 

Fu L, Wang R, Yin L, Shang X, Zhang R and Zhang P: CYFRA21-1 tests in the diagnosis of non-small cell lung cancer: A meta-analysis. Int J Biol Markers. 34:251–261. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Qin K, Chen Y, Long H, Chen J, Wang D, Chen L and Liang Z: The biomarkers and potential pathogenesis of lung cancer related cerebral hemorrhage. Medicine (Baltimore). 98:e156932019. View Article : Google Scholar : PubMed/NCBI

6 

Ogawa M, Mizugishi K, Ishiguro A, Koyabu Y, Imai Y, Takahashi R, Mikoshiba K and Aruga J: Rines/RNF180, a novel RING finger gene-encoded product, is a membrane-bound ubiquitin ligase. Genes Cells. 13:397–409. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zhang X, Zhang X, Sun B, Lu H, Wang D, Yuan X and Huang Z: Detection of aberrant promoter methylation of RNF180, DAPK1 and SFRP2 in plasma DNA of patients with gastric cancer. Oncol Lett. 8:1745–1750. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Han F, Sun LP, Liu S, Xu Q, Liang QY, Zhang Z, Cao HC, Yu J, Fan DM, Nie YZ, et al: Promoter methylation of RNF180 is associated with H. pylori infection and serves as a marker for gastric cancer and atrophic gastritis. Oncotarget. 7:24800–24809. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Cheung KF, Lam CN, Wu K, Ng EK, Chong WW, Cheng AS, To KF, Fan D, Sung JJ and Yu J: Characterization of the gene structure, functional significance, and clinical application of RNF180, a novel gene in gastric cancer. Cancer. 118:947–959. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Deng J, Liang H, Zhang R, Hou Y, Liu Y, Ying G, Pan Y and Hao X: Clinical and experimental role of ring finger protein 180 on lymph node metastasis and survival in gastric cancer. Br J Surg. 103:407–416. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Udali S, Guarini P, Ruzzenente A, Ferrarini A, Guglielmi A, Lotto V, Tononi P, Pattini P, Moruzzi S, Campagnaro T, et al: DNA methylation and gene expression profiles show novel regulatory pathways in hepatocellular carcinoma. Clin Epigenetics. 7:432015. View Article : Google Scholar : PubMed/NCBI

12 

Han F, Liu S, Jing J, Li H, Yuan Y and Sun LP: Identification of high-frequency methylation sites in RNF180 promoter region affecting expression and their relationship with prognosis of gastric cancer. Cancer Manag Res. 12:3389–3399. 2020. View Article : Google Scholar : PubMed/NCBI

13 

Akhurst T: Staging of non-small-cell lung cancer. PET Clin. 13:1–10. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Ottis P, Toure M, Cromm PM, Ko E, Gustafson JL and Crews CM: Assessing different E3 ligases for small molecule induced protein ubiquitination and degradation. ACS Chem Biol. 12:2570–2578. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Ning Y, Hui N, Qing B, Zhuo Y, Sun W, Du Y, Liu S, Liu K and Zhou J: ZCCHC10 suppresses lung cancer progression and cisplatin resistance by attenuating MDM2-mediated p53 ubiquitination and degradation. Cell Death Dis. 10:4142019. View Article : Google Scholar : PubMed/NCBI

17 

Deng J, Guo J, Guo X, Hou Y, Xie X, Sun C, Zhang R, Yu X and Liang H: Mediation of the malignant biological characteristics of gastric cancer cells by the methylated CpG islands in RNF180 DNA promoter. Oncotarget. 7:43461–43474. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Potabattula R, Dittrich M, Schorsch M, Hahn T, Haaf T and El Hajj N: Male obesity effects on sperm and next-generation cord blood DNA methylation. PLoS One. 14:e02186152019. View Article : Google Scholar : PubMed/NCBI

19 

Yang ZH, Dang YQ and Ji G: Role of epigenetics in transformation of inflammation into colorectal cancer. World J Gastroenterol. 25:2863–2877. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Sutton LP, Jeffreys SA, Phillips JL, Taberlay PC, Holloway AF, Ambrose M, Joo JE, Young A, Berry R, Skala M, et al: DNA methylation changes following DNA damage in prostate cancer cells. Epigenetics. 14:989–1002. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Xie XM, Deng JY, Hou YC, Cui JL, Wu WP, Ying GG, Dong QP, Hao XS and Liang H: Evaluating the clinical feasibility: The direct bisulfite genomic sequencing for examination of methylated status of E3 ubiquitin ligase RNF180 DNA promoter to predict the survival of gastric cancer. Cancer Biomark. 15:259–265. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Deng J, Liang H, Zhang R, Ying G, Xie X, Yu J, Fan D and Hao X: Methylated CpG site count of dapper homolog 1 (DACT1) promoter prediction the poor survival of gastric cancer. Am J Cancer Res. 4:518–527. 2014.PubMed/NCBI

23 

Deng J, Liang H, Ying G, Li H, Xie X, Yu J, Fan D and Hao X: Methylation of ras association domain protein 10 (RASSF10) promoter negative association with the survival of gastric cancer. Am J Cancer Res. 4:916–923. 2014.PubMed/NCBI

24 

Paun O, Verhoeven KJF and Richards CL: Opportunities and limitations of reduced representation bisulfite sequencing in plant ecological epigenomics. New Phytol. 221:738–742. 2019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu H, Yang P, Li X and Jia Y: Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer. Oncol Lett 20: 35, 2020.
APA
Liu, H., Yang, P., Li, X., & Jia, Y. (2020). Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer. Oncology Letters, 20, 35. https://doi.org/10.3892/ol.2020.11898
MLA
Liu, H., Yang, P., Li, X., Jia, Y."Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer". Oncology Letters 20.4 (2020): 35.
Chicago
Liu, H., Yang, P., Li, X., Jia, Y."Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer". Oncology Letters 20, no. 4 (2020): 35. https://doi.org/10.3892/ol.2020.11898
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Yang P, Li X and Jia Y: Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer. Oncol Lett 20: 35, 2020.
APA
Liu, H., Yang, P., Li, X., & Jia, Y. (2020). Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer. Oncology Letters, 20, 35. https://doi.org/10.3892/ol.2020.11898
MLA
Liu, H., Yang, P., Li, X., Jia, Y."Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer". Oncology Letters 20.4 (2020): 35.
Chicago
Liu, H., Yang, P., Li, X., Jia, Y."Ring finger protein 180 is associated with biological behavior and prognosis in patients with non‑small cell lung cancer". Oncology Letters 20, no. 4 (2020): 35. https://doi.org/10.3892/ol.2020.11898
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team