Open Access

Identification of UBE2T as an independent prognostic biomarker for gallbladder cancer

  • Authors:
    • Xuan Zhu
    • Tao Li
    • Xing Niu
    • Lijie Chen
    • Chunlin Ge
  • View Affiliations

  • Published online on: July 24, 2020     https://doi.org/10.3892/ol.2020.11903
  • Article Number: 44
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gallbladder cancer is the most common biliary tract malignant tumor, with unfavorable patient outcomes. The present study aimed to identify potential diagnostic or prognostic biomarkers for gallbladder cancer. To do so, differentially expressed genes in the gallbladder walls and tumor tissues of patients with gallbladder cancer were analyzed via microarray. Furthermore, a protein‑protein interaction network was constructed and genes with a degree score >10 were selected as hub genes. As ubiquitin conjugating enzyme E2T (UBE2T) was considered to be a hub gene, its expression was assessed via reverse transcription‑quantitative (RT‑q)PCR and immunohistochemistry (IHC). In addition, the association between UBE2T expression and the clinicopathological characteristics of patients with gallbladder cancer was analyzed using the χ2 test. Furthermore, all patients were divided into high‑ and low groups based on UBE2T expression level and overall survival analysis was performed. Univariate and multivariate Cox regression analyses were performed to determine whether UBE2T may serve as an independent risk factor for gallbladder cancer. The results demonstrated that UBE2T expression was upregulated in the gallbladder walls and tumor tissues of patients with gallbladder cancer. Furthermore, UBE2T expression level was confirmed to be upregulated following RT‑qPCR, and results from IHC demonstrated that UBE2T was predominantly expressed in the cytoplasm of gallbladder cancer cells. In addition, high UBE2T expression level was associated with clinical stage, T classification, N classification and M classification. The results from Univariate and multivariate analyses indicated that UBE2T expression level may be considered as an independent risk factor for gallbladder cancer. Taken together, the findings from this study suggested that high UBE2T expression level may contribute to the poor prognosis of patients with gallbladder cancer, and that UBE2T may act as an independent prognostic biomarker for these patients.
View Figures
View References

Related Articles

Journal Cover

October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu X, Li T, Niu X, Chen L and Ge C: Identification of UBE2T as an independent prognostic biomarker for gallbladder cancer. Oncol Lett 20: 44, 2020
APA
Zhu, X., Li, T., Niu, X., Chen, L., & Ge, C. (2020). Identification of UBE2T as an independent prognostic biomarker for gallbladder cancer. Oncology Letters, 20, 44. https://doi.org/10.3892/ol.2020.11903
MLA
Zhu, X., Li, T., Niu, X., Chen, L., Ge, C."Identification of UBE2T as an independent prognostic biomarker for gallbladder cancer". Oncology Letters 20.4 (2020): 44.
Chicago
Zhu, X., Li, T., Niu, X., Chen, L., Ge, C."Identification of UBE2T as an independent prognostic biomarker for gallbladder cancer". Oncology Letters 20, no. 4 (2020): 44. https://doi.org/10.3892/ol.2020.11903