Open Access

Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway

  • Authors:
    • Dingkun Feng
    • Min Liu
    • Yanting Liu
    • Xiaojin Zhao
    • Huan Sun
    • Xu Zheng
    • Jiabin Zhu
    • Fajun Shang
  • View Affiliations

  • Published online on: July 29, 2020     https://doi.org/10.3892/ol.2020.11928
  • Article Number: 67
  • Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Micheliolide (MCL), a sesquiterpene lactone isolated from Michelia compressa and Michelia champaca, has been used previously to inhibit the NF‑κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our knowledge, its underlying anticancer mechanism remains to be understood. The present study aimed to investigate the effects of MCL on human glioma U251MG cells and to determine the potential anticancer mechanism of action of MCL. From Cell Counting Kit‑8, colony formation assay, apoptosis assay and Confocal immunofluorescence imaging analysis, the results revealed that MCL significantly inhibited cell viability in vitro and induced cell apoptosis via activation of the cytochrome c/caspase‑dependent apoptotic pathway. In addition, MCL also suppressed cell invasion and metastasis via the wound healing and Transwell invasion assays. Furthermore, western blot and reverse transcription PCR analyses demonstrated that MCL significantly downregulated cyclooxygenase‑2 (COX‑2) expression levels, which may have partially occurred through the inactivation of the NF‑κB signaling pathway. In conclusion, the results of the present study indicated that MCL may inhibit glioma carcinoma growth by downregulating the NF‑κB/COX‑2 signaling pathway, which suggested that MCL may be a novel and alternative antitumor agent for the treatment of human glioma carcinoma.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Feng D, Liu M, Liu Y, Zhao X, Sun H, Zheng X, Zhu J and Shang F: Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway. Oncol Lett 20: 67, 2020.
APA
Feng, D., Liu, M., Liu, Y., Zhao, X., Sun, H., Zheng, X. ... Shang, F. (2020). Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway. Oncology Letters, 20, 67. https://doi.org/10.3892/ol.2020.11928
MLA
Feng, D., Liu, M., Liu, Y., Zhao, X., Sun, H., Zheng, X., Zhu, J., Shang, F."Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway". Oncology Letters 20.4 (2020): 67.
Chicago
Feng, D., Liu, M., Liu, Y., Zhao, X., Sun, H., Zheng, X., Zhu, J., Shang, F."Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway". Oncology Letters 20, no. 4 (2020): 67. https://doi.org/10.3892/ol.2020.11928