Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑&kappa;B signaling pathway

Feng,Dingkun; Liu,Min; Liu,Yanting; Zhao,Xiaojin; Sun,Huan; Zheng,Xu; Zhu,Jiabin; Shang,Fajun

doi:10.3892/ol.2020.11928

October-2020 Volume 20 Issue 4

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October-2020 Volume 20 Issue 4

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Article Open Access

Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF‑κB signaling pathway

Authors:
- Dingkun Feng
- Min Liu
- Yanting Liu
- Xiaojin Zhao
- Huan Sun
- Xu Zheng
- Jiabin Zhu
- Fajun Shang
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Affiliations: Department of Neurosurgery, The Affiliated Renhe Hospital, China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Neurology, Xinhua Hospital affiliated to Dalian University, Dalian, Liaoning 116021, P.R. China, Department of Neurosurgery, The First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, Hubei 443003, P.R. China, Department of Gastroenterology, The Affiliated Renhe Hospital, China Three Gorges University, Yichang, Hubei 443000, P.R. China

Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 67
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Published online on: July 29, 2020

https://doi.org/10.3892/ol.2020.11928
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Abstract

Micheliolide (MCL), a sesquiterpene lactone isolated from Michelia compressa and Michelia champaca, has been used previously to inhibit the NF‑κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our knowledge, its underlying anticancer mechanism remains to be understood. The present study aimed to investigate the effects of MCL on human glioma U251MG cells and to determine the potential anticancer mechanism of action of MCL. From Cell Counting Kit‑8, colony formation assay, apoptosis assay and Confocal immunofluorescence imaging analysis, the results revealed that MCL significantly inhibited cell viability in vitro and induced cell apoptosis via activation of the cytochrome c/caspase‑dependent apoptotic pathway. In addition, MCL also suppressed cell invasion and metastasis via the wound healing and Transwell invasion assays. Furthermore, western blot and reverse transcription PCR analyses demonstrated that MCL significantly downregulated cyclooxygenase‑2 (COX‑2) expression levels, which may have partially occurred through the inactivation of the NF‑κB signaling pathway. In conclusion, the results of the present study indicated that MCL may inhibit glioma carcinoma growth by downregulating the NF‑κB/COX‑2 signaling pathway, which suggested that MCL may be a novel and alternative antitumor agent for the treatment of human glioma carcinoma.

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