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miR‑375‑3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor‑1β

  • Authors:
    • Kunpeng Chang
    • Zhenxing Wei
    • Hua Cao

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  • Published online on: August 3, 2020     https://doi.org/10.3892/ol.2020.11941
  • Article Number: 80

  • Copyright : © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most frequently diagnosed head and neck cancers worldwide. Increasing evidence suggests that microRNAs (miRNAs/miRs) regulate the progression of tumorigenesis and the malignant behaviors of cancer cells. The aim of this study was to investigate the function and underlying mechanism of miR‑375‑3p in LSCC. The expression of miR‑375‑3p in LSCC tissues and cells was detected using reverse transcription‑quantitative PCR. The effects of miR‑375‑3p on the malignant phenotype of LSCC cells was determined using the Cell Counting Kit‑8 assay and flow cytometry. The targets of miR‑375‑3p were predicted using the miRDB database and confirmed by the luciferase reporter assay. The results of the present study demonstrated that miR‑375‑3p was downregulated in LSCC tissues and cell lines. Furthermore, overexpression of miR‑375‑3p significantly suppressed the proliferation and cell cycle progression of LSCC cells. Overexpression of miR‑375‑3p also increased LSCC cell apoptosis. Mechanistical analysis indicated that miR‑375‑3p bound the 3'‑untranslated region of the hepatocyte nuclear factor 1β (HNF1β) and decreased its expression in LSCC cells. Consistent with the role of HNF1β in glucose metabolism, overexpression of miR‑375‑3p significantly inhibited glucose consumption and lactate production in LSCC cells. Transfection with HNF1β notably reversed the inhibitory effect of miR‑375‑3p on the proliferation of LSCC cells. Collectively, these results indicate the tumor suppressive role of miR‑375‑3p in LSCC via HNF1β, suggesting that miR‑375‑3p may serve as a potential target in the treatment of LSCC.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Impact Factor: 2.9
  • Ranked Oncology
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  • CiteScore: 6.3
  • CiteScore Rank: Q2: #119/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.725
  • SCImago Journal Rank (SJR): 0.639
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Spandidos Publications style
Chang K, Wei Z and Cao H: miR‑375‑3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor‑1β. Oncol Lett 20: 80, 2020
APA
Chang, K., Wei, Z., & Cao, H. (2020). miR‑375‑3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor‑1β. Oncology Letters, 20, 80. https://doi.org/10.3892/ol.2020.11941
MLA
Chang, K., Wei, Z., Cao, H."miR‑375‑3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor‑1β". Oncology Letters 20.4 (2020): 80.
Chicago
Chang, K., Wei, Z., Cao, H."miR‑375‑3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor‑1β". Oncology Letters 20, no. 4 (2020): 80. https://doi.org/10.3892/ol.2020.11941