Open Access

Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy in a mouse model of esophageal cancer

  • Authors:
    • Yoshihiro Tanaka
    • Yasutoshi Murayama
    • Tatsuya Matsumoto
    • Hidemasa Kubo
    • Kyoichi Harada
    • Hisataka Matsuo
    • Takeshi Kubota
    • Kazuma Okamoto
    • Eigo Otsuji
  • View Affiliations

  • Published online on: August 4, 2020     https://doi.org/10.3892/ol.2020.11943
  • Article Number: 82
  • Copyright: © Tanaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

5‑Aminolevulinic acid‑mediated photodynamic therapy (ALA‑PDT) is a minimally invasive therapeutic modality used in the management of various cancers, but to a lesser extent for esophageal cancer (EC). The current study investigated the antitumor effects of ALA‑PDT. Human EC cells were treated with ALA, after which ALA‑induced fluorescence was examined under a fluorescence microscope. The cytotoxic effects of ALA‑PDT were assessed using three types of LEDs (blue, green and red) in vitro and in vivo. Subcutaneous tumor model mice was constructed with KYSE150 cells. ALA‑PDT was performed once a week for 4 weeks and tumor weights were measured. A popliteal lymph node (PLN) metastasis murine model was generated using KYSE150 cells. KYSE150 cells were inoculated into the left footpad of nude mice. ALA‑PDT was performed on the footpad once a week for 4 weeks. PLNs were then removed 3 weeks after the last treatment. The lymph nodes were evaluated by hematoxylin and eosin staining. Red fluorescence of protoporphyrin IX (PpIX) was observed in all EC cell lines. ALA‑PDT using LEDs exerted significant antitumor effects in vitro and in vivo. The antitumor effects of ALA‑PDT with blue LED were the strongest, followed by green and red LEDs. The number of metastasized PLNs was significantly smaller in the ALA‑PDT group (0%) than in the control group (37.5%). The present results indicated that ALA‑PDT is effective for EC.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Tanaka Y, Murayama Y, Matsumoto T, Kubo H, Harada K, Matsuo H, Kubota T, Okamoto K and Otsuji E: Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy in a mouse model of esophageal cancer. Oncol Lett 20: 82, 2020
APA
Tanaka, Y., Murayama, Y., Matsumoto, T., Kubo, H., Harada, K., Matsuo, H. ... Otsuji, E. (2020). Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy in a mouse model of esophageal cancer. Oncology Letters, 20, 82. https://doi.org/10.3892/ol.2020.11943
MLA
Tanaka, Y., Murayama, Y., Matsumoto, T., Kubo, H., Harada, K., Matsuo, H., Kubota, T., Okamoto, K., Otsuji, E."Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy in a mouse model of esophageal cancer". Oncology Letters 20.4 (2020): 82.
Chicago
Tanaka, Y., Murayama, Y., Matsumoto, T., Kubo, H., Harada, K., Matsuo, H., Kubota, T., Okamoto, K., Otsuji, E."Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy in a mouse model of esophageal cancer". Oncology Letters 20, no. 4 (2020): 82. https://doi.org/10.3892/ol.2020.11943