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p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone

  • Authors:
    • Shu Liu
    • Fan Ye
    • Dongqi Li
    • Chuanchun He
    • Hao He
    • Jing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Bone and Soft Tissue Tumors Research Center of Yunnan Province, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan 650118, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 86
    |
    Published online on: August 5, 2020
       https://doi.org/10.3892/ol.2020.11947
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Abstract

Giant cell tumor of bone (GCTB) is an intermediate (locally aggressive) bone tumor with a recurrence rate of >30% following surgery. GCTB recurrence is ultimately due to the proliferation of neoplastic stromal (NS) cells. However, the precise mechanism underlying the regulation of NS cell proliferation remains unknown. p62 protein is a multifunctional adaptor protein that exerts a positive role in bone tumors and metabolic bone diseases. In the present study, the mRNA and protein expression levels of p62 were detected by reverse transcription‑quantitative PCR and western blotting, respectively, in 8 paired fresh GCTB tumor tissues and adjacent normal cancellous bone tissues. The association between p62 expression level and patient prognosis was subsequently analyzed in 54 paraffin‑embedded tumor specimens by immunohistochemistry assay. NS cells were isolated from GCTB primary cell culture, and the role of p62 was evaluated using in vitro cell proliferation, migration and invasion assays. The results revealed that p62 mRNA and protein were overexpressed in tumor tissues. High p62 expression levels were significantly associated with the recurrence of GCTB (P=0.001). The patients in the high p62 expression group had shorter 5‑year recurrence‑free survival rates compared with the patients in the low p62 expression group (P<0.001). Cox regression analysis identified p62 expression as an independent prognostic indicator of the recurrence‑free survival of patients with GCTB (P<0.001). The in vitro experiments revealed that p62 downregulation inhibited NS cell proliferation, invasion and migration, and promoted apoptosis. In conclusion, it was found that p62 overexpression is associated with the recurrence of GCTB via the promotion of NS cell proliferation. Therefore, p62 could be a novel prognostic indicator, and a potential therapeutic target for GCTB.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Ye F, Li D, He C, He H and Zhang J: p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone. Oncol Lett 20: 86, 2020.
APA
Liu, S., Ye, F., Li, D., He, C., He, H., & Zhang, J. (2020). p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone. Oncology Letters, 20, 86. https://doi.org/10.3892/ol.2020.11947
MLA
Liu, S., Ye, F., Li, D., He, C., He, H., Zhang, J."p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone". Oncology Letters 20.4 (2020): 86.
Chicago
Liu, S., Ye, F., Li, D., He, C., He, H., Zhang, J."p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone". Oncology Letters 20, no. 4 (2020): 86. https://doi.org/10.3892/ol.2020.11947
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Ye F, Li D, He C, He H and Zhang J: p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone. Oncol Lett 20: 86, 2020.
APA
Liu, S., Ye, F., Li, D., He, C., He, H., & Zhang, J. (2020). p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone. Oncology Letters, 20, 86. https://doi.org/10.3892/ol.2020.11947
MLA
Liu, S., Ye, F., Li, D., He, C., He, H., Zhang, J."p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone". Oncology Letters 20.4 (2020): 86.
Chicago
Liu, S., Ye, F., Li, D., He, C., He, H., Zhang, J."p62 overexpression promotes neoplastic stromal cell proliferation and is associated with the recurrence of giant cell tumor of bone". Oncology Letters 20, no. 4 (2020): 86. https://doi.org/10.3892/ol.2020.11947
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