Open Access

Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway

  • Authors:
    • Xiangdao Cai
    • Bang Hu
    • Sheng Liu
    • Maolin Liu
    • Yunhe Huang
    • Peng Lei
    • Zhi Zhang
    • Zhiwei He
    • Linquan Zhang
    • Rimao Huang
  • View Affiliations

  • Published online on: August 12, 2020     https://doi.org/10.3892/ol.2020.11972
  • Article Number: 111
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Drug resistance leads to tumor relapse and further progression during chemotherapy in lung cancer. Close homolog of L1 (CHL1) has been identified as a tumor suppressor in most malignancies. However, to the best of our knowledge, whether CHL1 mediates chemoresistance remains unknown. The present study observed that CHL1 was significantly downregulated in cisplatin (DDP)‑resistant cells (A549/DDP) and paclitaxel (PTX)‑resistant cells (A549/PTX) compared with A549 cells. When treated with or without DDP and PTX, silencing of CHL1 in A549 cells promoted the cell survival rate and clone formation, and decreased apoptosis. Whereas overexpression of CHL1 in A549/DDP and A549/PTX cells impeded the cell survival and clone formation and promoted apoptosis. Additionally, CHL1 overexpression enhanced the chemosensitivity of A549/DDP cells to DDP in vivo. Notably, the chemoresistance induced by CHL1 depletion was reversed by the Akt inhibitor SC66 in A549 cells. The results of the present study demonstrated that CHL1 enhanced sensitivity of lung cancer cells by suppressing the Akt pathway, which suggested that CHL1 may be a potential target for overcoming chemoresistance in lung cancer.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cai X, Hu B, Liu S, Liu M, Huang Y, Lei P, Zhang Z, He Z, Zhang L, Huang R, Huang R, et al: Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway. Oncol Lett 20: 111, 2020
APA
Cai, X., Hu, B., Liu, S., Liu, M., Huang, Y., Lei, P. ... Huang, R. (2020). Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway. Oncology Letters, 20, 111. https://doi.org/10.3892/ol.2020.11972
MLA
Cai, X., Hu, B., Liu, S., Liu, M., Huang, Y., Lei, P., Zhang, Z., He, Z., Zhang, L., Huang, R."Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway". Oncology Letters 20.4 (2020): 111.
Chicago
Cai, X., Hu, B., Liu, S., Liu, M., Huang, Y., Lei, P., Zhang, Z., He, Z., Zhang, L., Huang, R."Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway". Oncology Letters 20, no. 4 (2020): 111. https://doi.org/10.3892/ol.2020.11972