Cyto‑biological effects of microRNA‑424‑5p on human colorectal cancer cells

Yan,Weitao; Jiang,Xia; Wang,Guiqi; Li,Wei; Zhai,Congjie; Chen,Shihao; Shang,Fangjian; Zhao,Zengren; Yu,Weifang

doi:10.3892/ol.2020.11982

Cyto‑biological effects of microRNA‑424‑5p on human colorectal cancer cells

Authors:
- Weitao Yan
- Xia Jiang
- Guiqi Wang
- Wei Li
- Congjie Zhai
- Shihao Chen
- Fangjian Shang
- Zengren Zhao
- Weifang Yu
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Affiliations: Department of General Surgery, Hebei Key Laboratory of Colorectal Cancer Precision Diagnosis and Treatment, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, P.R. China, Department of Endoscopy Center, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, P.R. China
Published online on: August 13, 2020 https://doi.org/10.3892/ol.2020.11982
Article Number: 120
Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miR)‑424‑5p is overexpressed in colorectal cancer (CRC); however, its role, clinical significance and underlying molecular mechanism have remained to be fully elucidated. The aim of the present study was to investigate the roles of miR‑424‑5p in CRC and the underlying mechanisms. It was demonstrated that miR‑424‑5p is overexpressed in CRC, based on bioinformatics analysis using The Cancer Genome Atlas TCGA and analysis of tissue samples from patients with CRC from The First Hospital of Hebei Medical University, and the expression of miR‑424‑5p was associated with the depth of invasion and Dukes' staging. In CRC cells, the oncogenic roles of miR‑424‑5p were also verified by Cell Counting Kit‑8, wound healing and Transwell assays. To identify target genes, all transcripts were compared between miR‑424‑5p mimic‑transfected SW480 cells and mimic control cells by transcriptome sequencing. Subsequently, the differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The DEGs were revealed to be signiﬁcantly enriched in the GO terms ‘serine hydrolase activity,’ ‘serine‑type peptidase activity’ and ‘serine‑type endopeptidase activity’. KEGG signaling pathway analysis indicated that the DEGs were signiﬁcantly enriched in ‘endocytosis’, ‘regulation of actin cytoskeleton’, ‘Wnt signaling pathway’ and ‘ubiquitin‑mediated proteolysis signaling pathway’. These results suggested that miR‑424‑5p is a potential target in the treatment of CRC.

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